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Abstract:

Cell wall changes in vegetative and suffultory cells (SCs) and in oogonial structures from Oedogonium bharuchae N. D. Kamat f. minor Vélez were characterized using monoclonal antibodies against several carbohydrate epitopes. Vegetative cells and SCs develop only a primary cell wall (PCW), whereas mature oogonial cells secrete a second wall, the oogonium cell wall (OCW). Based on histochemical and immunolabeling results, (1→4)-β-glucans in the form of crystalline cellulose together with a variable degree of Me-esterified homogalacturonans (HGs) and hydroxyproline-rich glycoprotein (HRGP) epitopes were detected in the PCW. The OCW showed arabinosides of the extensin type and low levels of arabinogalactan-protein (AGP) glycans but lacked cellulose, at least in its crystalline form. Surprisingly, strong colabeling in the cytoplasm of mature oogonia cells with three different antibodies (LM-5, LM-6, and CCRC-M2) was found, suggesting the presence of rhamnogalacturonan I (RG-I)-like structures. Our results are discussed relating the possible functions of these cell wall epitopes with polysaccharides and O-glycoproteins during oogonium differentiation. This study represents the first attempt to characterize these two types of cell walls in O. bharuchae, comparing their similarities and differences with those from other green algae and land plants. This work represents a contribution to the understanding of how cell walls have evolved from simple few-celled to complex multicelled organisms. © 2008 Phycological Society of America.

Registro:

Documento: Artículo
Título:Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)
Autor:Estevez, J.M.; Leonardi, P.I.; Alberghina, J.S.
Filiación:Carnegie Institution, Plant Biology, Stanford University, Stanford, CA 94305, United States
Departamento de Biología, Bioquímica Y Farmacia, Universidad Nacional del Sur, CERZOS-CONICET, 8000 Bahía Blanca, Argentina
Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria - Pabellón 2, 1428 Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria - Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Cell wall; Cellulose; Green algae; HRGP; Oedogonium bharuchae f. minor; Oogonium development; Pectins; Chlorophyta; Embryophyta; Oedogoniales; Oedogonium bharuchae; Oedogonium bharuchae f. minor
Año:2008
Volumen:44
Número:5
Página de inicio:1257
Página de fin:1268
DOI: http://dx.doi.org/10.1111/j.1529-8817.2008.00568.x
Título revista:Journal of Phycology
Título revista abreviado:J. Phycol.
ISSN:00223646
CODEN:JPYLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v44_n5_p1257_Estevez

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Citas:

---------- APA ----------
Estevez, J.M., Leonardi, P.I. & Alberghina, J.S. (2008) . Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta). Journal of Phycology, 44(5), 1257-1268.
http://dx.doi.org/10.1111/j.1529-8817.2008.00568.x
---------- CHICAGO ----------
Estevez, J.M., Leonardi, P.I., Alberghina, J.S. "Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)" . Journal of Phycology 44, no. 5 (2008) : 1257-1268.
http://dx.doi.org/10.1111/j.1529-8817.2008.00568.x
---------- MLA ----------
Estevez, J.M., Leonardi, P.I., Alberghina, J.S. "Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta)" . Journal of Phycology, vol. 44, no. 5, 2008, pp. 1257-1268.
http://dx.doi.org/10.1111/j.1529-8817.2008.00568.x
---------- VANCOUVER ----------
Estevez, J.M., Leonardi, P.I., Alberghina, J.S. Cell wall carbohydrate epitopes in the green alga Oedogonium bharuchae f. minor (Oedogoniales, Chlorophyta). J. Phycol. 2008;44(5):1257-1268.
http://dx.doi.org/10.1111/j.1529-8817.2008.00568.x