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Abstract:

Carrageenans biosynthesized by gametophytic and tetrasporic plants of seaweeds belonging to the Gigartinaceae and Phyllophoraceae are different: gametophytes produce carrageenans of the kappa family, whereas lambda-carrageenans are extracted from tetrasporophytes. For Gigartina skottsbergii Setchell and Gardner and Gymnogongrus torulosus Hooker et Harvey, mature cystocarps were isolated and carrageenans were extracted. Structural determination by methylation analysis, Fourier transform infrared spectroscopy, and 13C-NMR spectroscopy showed that they were kappa/iota-carrageenans. For the extract obtained from cystocarps of Gigartina skottsbergii with water at room temperature, the ratio kappa:iota was 1:0.30 and at 90°C was 1:0.43; significant amounts of precursors were also present. The extract obtained from cystocarps of Gymnogongrus torulosus at 90°C showed prevalence of iota-carrageenans (ratio kappa:iota 1:1.21). These extracts are similar to the polysaccharides produced by gametophytes of these seaweeds. For Gigartina skottsbergii, it was possible to separate the pericarpic tissue from the carposporophyte. Thus, they were extracted separately, and the carrageenans isolated were studied as described before, obtaining similar conclusions. These results clearly show that whereas the carposporophytes are located inside the cystocarp, they produce carrageenans of the kappa family despite of being diploid cells.

Registro:

Documento: Artículo
Título:Carrageenans biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii (Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae)
Autor:Estevez, J.M.; Ciancia, M.; Cerezo, A.S.
Filiación:Departamento de Química Orgánica (CIHIDECAR-CONICET), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria - Pabellón 2, 1428 Buenos Aires, Argentina
National Research Council of Argentina(CONICET), Argentina
Palabras clave:Carposporophyte; Carrageenan structure; Cystocarps; Gametophyte; Gigartina skottsbergii (Gigartinaceae); Gymnogongrus torulosus (Phyllophoraceae); Kappa/iota-carrageenan; Lambda-carrageenan; carrageenan; gametophyte; red alga; algae; Eukaryota; Gigartina; Gigartina skottsbergii; Gigartinaceae; Gymnogongrus; Gymnogongrus torulosus; Halophila; Phyllophoraceae; Rhodophyta; Spermatophyta
Año:2002
Volumen:38
Número:2
Página de inicio:344
Página de fin:350
DOI: http://dx.doi.org/10.1046/j.1529-8817.2002.01109.x
Título revista:Journal of Phycology
Título revista abreviado:J. Phycol.
ISSN:00223646
CODEN:JPYLA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223646_v38_n2_p344_Estevez

Referencias:

  • Bert, M., Ben Said, R., Deslandes, E., Cosson, J., Carrageenans from tetrasporophytic and gametophytic stages of Cystoclonium purpureum (1989) Phytochemistry, 28, pp. 71-72
  • Buschmann, A.H., Correa, J.A., Westermeier, R., Recent advances in the understanding of the biological basis for Gigartina skottsbergii (Rhodophyta) cultivation in Chile (1999) Hydrobiologia, 398-399, pp. 427-434
  • Cases, M.R., Cerezo, A.S., Stortz, C.A., Separation and quantitation of enantiomeric galactoses and their mono-O-methylethers as their diastereomeric acetylated 1-deoxy-1-(2-hydroxypropylamino)alditols (1995) Carbohydr. Res., 269, pp. 333-341
  • Chen, L.C.-M., McLachlan, J., Neish, A.C., Shacklock, P.F., The ratio of kappa- to lambda-carrageenan in nuclear phases of rhodophycean algae Chondrus crispus and Gigartina stellata (1973) J. Mar. Biol. Assoc. U.K., 53, pp. 11-16
  • Ciucanu, I., Kerek, F., A simple and rapid method for the permethylation of carbohydrates (1984) Carbohydr. Res., 134, pp. 209-217
  • Ciancia, M., Matulewicz, M.C., Cerezo, A.S., L-Galactose containing galactans from the carrageenophyte Gigartina skottsbergii (1993) Phytochemistry, 34, pp. 1541-1543
  • Ciancia, M., Matulewicz, M.C., Finch, P., Cerezo, A.S., Determination of the structures of cystocarpic carrageenans from Gigartina skottsbergii (1993) Carbohydr. Res., 238, pp. 241-248
  • Ciancia, M., Matulewicz, M.C., Cerezo, A.S., L-Galactose-containing carrageenans from cystocarpic Gigartina skottsbergii (1997) Phytochemistry, 45, pp. 1009-1013
  • Craigie, J., Cell walls (1990) Biology of the Red Algae, pp. 221-257. , Cole, K. & Sheath, R. [Eds.]. Cambridge University Press, Cambridge, England
  • DiNinno, V., McCandless, E.L., The chemistry and immunochemistry of carrageenans from Eucheuma and related species (1978) Carbohydr. Res., 66, pp. 85-93
  • Dodgson, K.S., Price, R.G., A note on the determination of the ester sulphate content of sulphated polysaccharides (1962) Biochem. J., 84, pp. 106-110
  • Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method of determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
  • Estevez, J.M., Ciancia, M., Cerezo, A.S., The system of low-molecular-weight carrageenans and agaroids from the room-temperature-extracted fraction of Kappaphycus alvarezii (2000) Carbohydr. Res., 325, pp. 287-299
  • Estevez, J.M., Ciancia, M., Cerezo, A.S., DL-Galactan hybrids and agarans from gametophytes of the red seaweed Gymnogongrus torulosus (2001) Carbohydr. Res., 331, pp. 27-41
  • Falshaw, R., Furneaux, R.H., Carrageenans from tetrasporic stage of Gigartina decipiens (Gigartinaceae, Rhodophyta) (1994) Carbohydr. Res., 252, pp. 171-182
  • Flores, M.L., Stortz, C.A., Rodriguez, M.C., Cerezo, A.S., Studies on the skeletal cell wall and cuticle of the cystocarpic stage of the red seaweeds Iridaea undulosa Bory (1997) Bot. Mar., 40, pp. 411-419
  • Glicksman, M., Red seaweed extracts (1983) Food Hydrocolloids, 2, pp. 73-114. , Glicksman, M. [Ed.]. CRC Press, Boca Raton, Florida
  • Gordon-Mills, E.M., McCandless, E.L., Carrageenans in the cell walls of Chondrus crispus (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody (1975) Phycologia, 14, pp. 275-281
  • Greer, C.W., Yaphe, W., Characterization of hybrid (beta-kappa-gamma) carrageenan from Eucheuma gelatinae J. Agardh (Rhodophyta, Solieriaceae) using carrageenases, infrared and 13C-nuclear magnetic resonance spectroscopy (1984) Bot. Mar., 28, pp. 473-478
  • Hommersand, M.H., Fredericq, S., Sexual reproduction and cystocarp development (1990) Biology of the Red Algae, pp. 305-346. , Cole, K. & Sheath, R. [Eds.]. Cambridge University Press, Cambridge, England
  • Lestang Bremond, G., Quillet, M., Bremond, M., λ-carrageenans in the gametophytes of Chondrus crispus (1987) Phytochemistry, 26, pp. 1705-1707
  • Matulewicz, M.C., Ciancia, M., Noseda, M.D., Cerezo, A.S., The carrageenan system from tetrasporic and cystocarpic stages of Gigartina skottsbergii (1989) Phytochemistry, 28, pp. 2937-2941
  • McCandless, E.L., Craigie, J.S., Walter, J.A., Carrageenans in the gametophytic and sporophytic stages of Chondrus crispus (1973) Planta (Berl.), 112, pp. 201-212
  • McCandless, E.L., Craigie, J.S., Sulphated polysaccharides in red and brown algae (1979) Annu. Rev. Plant Physiol., 30, pp. 41-53
  • McCandless, E.L., West, J.A., Guiry, M.D., Carrageenan patterns in the Phyllophoraceae (1982) Biochem. Syst. Ecol., 10, pp. 275-284
  • McCandless, E.L., West, J.A., Guiry, M.D., Carrageenan patterns in the Gigartinaceae (1983) Biochem. Syst. Ecol., 11, pp. 175-182
  • Morrison, I.M., Hydrolysis of plant cell walls with trifluoroacetic acid (1988) Phytochemistry, 27, pp. 1097-1100
  • Percival, E., Carbohydrate sulphates of the rhodophyceae (1978) ACS Symp. Ser. 77, pp. 213-224. , Schweigr, R. G. [Ed.]. Am. Chem. Soc., Washington, DC
  • Pickmere, S.E., Parsons, M.J., Bailey, R.W., Composition of Gigartina carrageenans in relation to sporophyte and gametophyte stages of the life cycle (1973) Phytochemistry, 12, pp. 2441-2444
  • Rees, D.A., Estimation of the relative amounts of isomeric sulphate esters in some sulphated polysaccharides (1961) J. Chem. Soc., pp. 5168-5171
  • Santelices, B., Correa, J.A., Aedo, D., Flores, V., Hormazábal, M., Sánchez, P., Convergent biological process in coalescent Rhodophyta (1999) J. Phycol., 35, pp. 1127-1149
  • Seymour, F.R., Knapp, R.D., Bishop, S.H., Determination of the structure of dextran by 13C-nuclear magnetic resonance spectroscopy (1976) Carbohydr. Res., 51, pp. 179-194
  • Stancioff, D.J., Stanley, N.F., Infrared and chemical studies on algal polysaccharides (1969) Proc. Int. Seaweed Symp., 6, pp. 595-609
  • Stevenson, T.T., Furneaux, R.H., Chemical methods for the analysis of sulphated galactans from red algae (1991) Carbohydr. Res., 210, pp. 277-298
  • Stortz, C.A., Matulewicz, M.C., Cerezo, A.S., Separation and identification of O-acetyl-O-methyl-galactonitriles by gas-liquid chromatography and mass spectrometry (1982) Carbohydr. Res., 111, pp. 31-39
  • Stortz, C.A., Cerezo, A.S., The system of carrageenans from cystocarpic and tetrasporic stages from Iridaea undulosa: Fractionation with potassium chloride and methylation analysis of the fractions (1993) Carbohydr. Res., 242, pp. 217-227
  • Stortz, C.A., Bacon, B.E., Cherniak, R., Cerezo, A.S., High-field NMR spectroscopy of cystocarpic and tetrasporic carrageenans from Iridaea undulosa (1994) Carbohydr. Res., 261, pp. 317-326
  • Usov, A.I., Shashkov, A.S., Polysaccharides of algae. 34. Detection of iota-carrageenan in Phyllophora brodiaei (Turn.) J. Ag. (Rhodophyta) using 13C-NMR spectroscopy (1985) Bot. Mar., 28, pp. 367-373
  • Yaphe, W., Arsenault, G.P., Improved resorcinol reagent for the determination of fructose, and of 3,6-anhydrogalactose in polysaccharides (1965) Anal. Biochem., 13, pp. 143-148
  • Waaland, J.R., Differences in carrageenan in gametophytes and tetrasporophytes of red algae (1975) Phytochemistry, 14, pp. 1359-1362

Citas:

---------- APA ----------
Estevez, J.M., Ciancia, M. & Cerezo, A.S. (2002) . Carrageenans biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii (Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae). Journal of Phycology, 38(2), 344-350.
http://dx.doi.org/10.1046/j.1529-8817.2002.01109.x
---------- CHICAGO ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. "Carrageenans biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii (Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae)" . Journal of Phycology 38, no. 2 (2002) : 344-350.
http://dx.doi.org/10.1046/j.1529-8817.2002.01109.x
---------- MLA ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. "Carrageenans biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii (Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae)" . Journal of Phycology, vol. 38, no. 2, 2002, pp. 344-350.
http://dx.doi.org/10.1046/j.1529-8817.2002.01109.x
---------- VANCOUVER ----------
Estevez, J.M., Ciancia, M., Cerezo, A.S. Carrageenans biosynthesized by carposporophytes of red seaweeds Gigartina skottsbergii (Gigartinaceae) and Gymnogongrus torulosus (Phyllophoraceae). J. Phycol. 2002;38(2):344-350.
http://dx.doi.org/10.1046/j.1529-8817.2002.01109.x