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Abstract:

Objectives: β-Escin, one of the constituents of Aesculus hippocastanum L. (Hippocastanaceae) seed extract (AH), inhibits NF-κB activation, which plays an important role in HSV-1 replication. The aim was to examine the antiherpetic activity of β-escin and AH, as well as their effect on the activation of NF-κB and AP-1 and cytokine secretion in epithelial cells and macrophages. Methods: Cell viability was evaluated using MTT assay, and antiviral and virucidal activity was determined by plaque assay. The effect on NF-κB and AP-1 signalling pathways activation was determined by a luciferase reporter assay, and cytokine production was measured by ELISA. Key findings: β-Escin and AH had virucidal and anti-HSV-1 activities, and the antiviral activity was discovered for other enveloped viruses (VSV and Dengue). Moreover, β-escin and AH significantly reduced NF-κB and AP-1 activation and cytokine production in macrophages stimulated with HSV-1 and TLRs ligands. However, an enhanced activation of these pathways and an increase in the levels of pro-inflammatory cytokines in β-escin and AH-treated HSV-1-infected epithelial cells were found. Conclusions: This study demonstrates virucidal and broad-spectrum antiviral activities for β escin and AH. Besides, β-escin and AH modulate cytokine production depending on the stimuli (viral or non-viral) and the cell type under study. © 2018 Royal Pharmaceutical Society

Registro:

Documento: Artículo
Título:Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract
Autor:Michelini, F.M.; Alché, L.E.; Bueno, C.A.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Virología, Buenos Aires, Argentina
CONICET – Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Palabras clave:Aesculus hippocastanum; antiviral; immunomodulatory; medicinal plants; β-escin; Aesculus hippocastanum extract; beta escin; immunoglobulin enhancer binding protein; toll like receptor; transcription factor AP 1; antivirus agent; autacoid; cytokine; escin; immunoglobulin enhancer binding protein; immunologic factor; plant extract; transcription factor AP 1; animal cell; antiviral activity; Article; cell viability; controlled study; cytokine production; Dengue virus; epithelium cell; herpes simplex; human; Human alphaherpesvirus 1; human cell; immunomodulation; inflammation; macrophage; nonhuman; Vesiculovirus; A-549 cell line; Aesculus; animal; chemistry; drug effect; immunology; isolation and purification; metabolism; mouse; pathogenicity; virology; virus; A549 Cells; Aesculus; Animals; Antiviral Agents; Cytokines; Dengue Virus; Epithelial Cells; Escin; Herpesvirus 1, Human; Humans; Immunologic Factors; Inflammation Mediators; Macrophages; Mice; NF-kappa B; Plant Extracts; Transcription Factor AP-1; Vesiculovirus; Viruses
Año:2018
Volumen:70
Número:11
Página de inicio:1561
Página de fin:1571
DOI: http://dx.doi.org/10.1111/jphp.13002
Título revista:Journal of Pharmacy and Pharmacology
Título revista abreviado:J. Pharm. Pharmacol.
ISSN:00223573
CODEN:JPPMA
CAS:beta escin, 64156-26-9; toll like receptor, 409141-78-2; escin, 6805-41-0, 8067-31-0; Antiviral Agents; Cytokines; Escin; Immunologic Factors; Inflammation Mediators; NF-kappa B; Plant Extracts; Transcription Factor AP-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223573_v70_n11_p1561_Michelini

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Citas:

---------- APA ----------
Michelini, F.M., Alché, L.E. & Bueno, C.A. (2018) . Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract. Journal of Pharmacy and Pharmacology, 70(11), 1561-1571.
http://dx.doi.org/10.1111/jphp.13002
---------- CHICAGO ----------
Michelini, F.M., Alché, L.E., Bueno, C.A. "Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract" . Journal of Pharmacy and Pharmacology 70, no. 11 (2018) : 1561-1571.
http://dx.doi.org/10.1111/jphp.13002
---------- MLA ----------
Michelini, F.M., Alché, L.E., Bueno, C.A. "Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract" . Journal of Pharmacy and Pharmacology, vol. 70, no. 11, 2018, pp. 1561-1571.
http://dx.doi.org/10.1111/jphp.13002
---------- VANCOUVER ----------
Michelini, F.M., Alché, L.E., Bueno, C.A. Virucidal, antiviral and immunomodulatory activities of β-escin and Aesculus hippocastanum extract. J. Pharm. Pharmacol. 2018;70(11):1561-1571.
http://dx.doi.org/10.1111/jphp.13002