Artículo

Kezunovic, N.; Hyde, J.; Simon, C.; Urbano, F.J.; Keith Williams, D.; Garcia-Rill, E. "Gamma band activity in the developing parafascicular nucleus" (2012) Journal of Neurophysiology. 107(3):772-784
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Abstract:

The parafascicular nucleus (Pf) receives cholinergic input from the pedunculopontine nucleus, part of the reticular activating system involved in waking and rapid eye movement (REM) sleep, and sends projections to the cortex. We tested the hypothesis that Pf neurons fire maximally at gamma band frequency (30-90 Hz), that this mechanism involves highthreshold voltage-dependent P/Q-and N-type calcium channels, and that this activity is enhanced by the cholinergic agonist carbachol (CAR). Patch-clamped 9-to 25-day-old rat Pf neurons (n = 299) manifested a firing frequency plateau at gamma band when maximally activated (31.5 ± 1.5 Hz) and showed gamma oscillations when voltage-clamped at holding potentials above -20 mV, and the frequency of the oscillations increased significantly with age (24.6 ± 3.8 vs. 51.6 ± 4.4 Hz, P < 0.001) but plateaued at gamma frequencies. Cells exposed to CAR showed significantly higher frequencies early in development compared with those without CAR (24.6 ± 3.8 vs. 41.7 ± 4.3 Hz, P < 0.001) but plateaued with age. The P/Q-type calcium channel blocker ω-agatoxin-IVA (ω-Aga) blocked gamma oscillations, whereas the N-type blocker ω-conotoxin-GVIA (ω-CgTx) only partially decreased the power spectrum amplitude of gamma oscillations. The blocking effect of ω-Aga on P/Q-type currents and ω-CgTx on N-type currents was consistent over age. We conclude that P/Q-and N-type calcium channels appear to mediate Pf gamma oscillations during development. We hypothesize that the cholinergic input to the Pf could activate these cells to oscillate at gamma frequency, and perhaps relay these rhythms to cortical areas, thus providing a stable high-frequency state for "nonspecific" thalamocortical processing. © 2012 the American Physiological Society.

Registro:

Documento: Artículo
Título:Gamma band activity in the developing parafascicular nucleus
Autor:Kezunovic, N.; Hyde, J.; Simon, C.; Urbano, F.J.; Keith Williams, D.; Garcia-Rill, E.
Filiación:Center for Translational Neuroscience, Departments of Neurobiology and Developmental Sciences and Biometry, University of Arkansas for Medical Sciences, Little Rock, AR, United States
Instituto de Fisiología, Biología Molecular y Neurociencias, Consejo Nacional de Investigaciones Científicas y Técnicas, University of Buenos Aires, Buenos Aires, Argentina
Palabras clave:ω-agatoxin IVA; ω-conotoxin-GVIA; Arousal; Calcium currents; Carbachol; Gamma oscillations; calcium channel N type; calcium channel P type; calcium channel Q type; carbachol; omega agatoxin IVA; omega agatoxin VIA; voltage dependent anion channel; aging; animal cell; animal experiment; article; brain cortex; brain nerve cell; calcium signaling; cholinergic system; controlled study; gamma rhythm; nerve potential; nonhuman; patch clamp; priority journal; rat; thalamus parafascicular nucleus; Animals; Calcium Channel Blockers; Calcium Channels, N-Type; Carbachol; Cholinergic Agonists; Intralaminar Thalamic Nuclei; Membrane Potentials; omega-Agatoxin IVA; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley
Año:2012
Volumen:107
Número:3
Página de inicio:772
Página de fin:784
DOI: http://dx.doi.org/10.1152/jn.00677.2011
Título revista:Journal of Neurophysiology
Título revista abreviado:J. Neurophysiol.
ISSN:00223077
CODEN:JONEA
CAS:carbachol, 462-58-8, 51-83-2; Calcium Channel Blockers; Calcium Channels, N-Type; Carbachol, 51-83-2; Cholinergic Agonists; omega-Agatoxin IVA; voltage-dependent calcium channel (P-Q type)
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223077_v107_n3_p772_Kezunovic

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Citas:

---------- APA ----------
Kezunovic, N., Hyde, J., Simon, C., Urbano, F.J., Keith Williams, D. & Garcia-Rill, E. (2012) . Gamma band activity in the developing parafascicular nucleus. Journal of Neurophysiology, 107(3), 772-784.
http://dx.doi.org/10.1152/jn.00677.2011
---------- CHICAGO ----------
Kezunovic, N., Hyde, J., Simon, C., Urbano, F.J., Keith Williams, D., Garcia-Rill, E. "Gamma band activity in the developing parafascicular nucleus" . Journal of Neurophysiology 107, no. 3 (2012) : 772-784.
http://dx.doi.org/10.1152/jn.00677.2011
---------- MLA ----------
Kezunovic, N., Hyde, J., Simon, C., Urbano, F.J., Keith Williams, D., Garcia-Rill, E. "Gamma band activity in the developing parafascicular nucleus" . Journal of Neurophysiology, vol. 107, no. 3, 2012, pp. 772-784.
http://dx.doi.org/10.1152/jn.00677.2011
---------- VANCOUVER ----------
Kezunovic, N., Hyde, J., Simon, C., Urbano, F.J., Keith Williams, D., Garcia-Rill, E. Gamma band activity in the developing parafascicular nucleus. J. Neurophysiol. 2012;107(3):772-784.
http://dx.doi.org/10.1152/jn.00677.2011