Most physiological processes in mammals are synchronized to the daily light:dark cycle by a circadian clock located in the hypothalamic suprachiasmatic nucleus. Signal transduction of light-induced phase advances of the clock is mediated through a neuronal nitric oxide synthase-guanilyl cyclase pathway. We have employed a novel nitric oxide-donor, N-nitrosomelatonin, to enhance the photic synchronization of circadian rhythms in hamsters. The intraperitoneal administration of this drug before a sub-saturating light pulse at circadian time 18 generated a twofold increase of locomotor rhythm phase-advances, having no effect over saturating light pulses. This potentiation was also obtained even when inhibiting suprachiasmatic nitric oxide synthase activity. However, N-nitrosomelatonin had no effect on light-induced phase delays at circadian time 14. The photic-enhancing effects were correlated with an increased suprachiasmatic immunoreactivity of FBJ murine osteosarcoma viral oncogene and period1. Moreover, in vivo nitric oxide release by N-nitrosomelatonin was verified by measuring nitrate and nitrite levels in suprachiasmatic nuclei homogenates. The compound also accelerated resynchronization to an abrupt 6-h advance in the light:dark cycle (but not resynchronization to a 6-h delay). Here, we demonstrate the chronobiotic properties of N-nitrosomelatonin, emphasizing the importance of nitric oxide-mediated transduction for circadian phase advances. © 2013 International Society for Neurochemistry.
Documento: | Artículo |
Título: | N-nitrosomelatonin enhances photic synchronization of mammalian circadian rhythms |
Autor: | Baidanoff, F.M.; Plano, S.A.; Doctorovich, F.; Suárez, S.A.; Golombek, D.A.; Chiesa, J.J. |
Filiación: | Laboratorio de Cronobiología Departamento de Ciencia y Tecnología Universidad Nacional de Quilmes/CONICET Bernal Argentina Departamento de Química Inorgánica Analítica y Química Física/INQUIMAE-CONICET Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Ciudad Universitaria Buenos Aires Argentina |
Idioma: | Inglés |
Palabras clave: | Circadian; Jet lag; N-nitrosomelatonin; Nitric oxide; Suprachiasmatic nucleus |
Año: | 2013 |
DOI: | http://dx.doi.org/10.1111/jnc.12613 |
Título revista: | Journal of Neurochemistry |
Título revista abreviado: | J. Neurochem. |
ISSN: | 00223042 |
CODEN: | JONRA |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00223042_v_n_p_Baidanoff |