Goitia, B.; Raineri, M.; González, L.E.; Rozas, J.L.; Garcia-Rill, E.; Bisagno, V.; Urbano, F.J. "Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei" (2013) Journal of Neurochemistry. 124(5):602-612
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Methylphenidate (MPH) is widely used to treat children and adolescents diagnosed with attention deficit/hyperactivity disorder. Although MPH shares mechanistic similarities to cocaine, its effects on GABAergic transmission in sensory thalamic nuclei are unknown. Our objective was to compare cocaine and MPH effects on GABAergic projections between thalamic reticular and ventrobasal (VB) nuclei. Mice (P18-30) were subjected to binge-like cocaine and MPH acute and sub-chronic administrations. Cocaine and MPH enhanced hyperlocomotion, although sub-chronic cocaine-mediated effects were stronger than MPH effects. Cocaine and MPH sub-chronic administration altered paired-pulse and spontaneous GABAergic input differently. The effects of cocaine on evoked paired-pulse GABA-mediated currents changed from depression to facilitation with the duration of the protocols used, while MPH induced a constant increase throughout the administration protocols. Thalamic reticular nucleus GAD67 and VB Ca V3.1 protein levels were measured using western blot to better understand their link to increased GABA release. Both proteins were increased by sub-chronic administration of cocaine. MPH showed effects on GABAergic transmission that seems less disruptive than cocaine. Unique effects of cocaine on postsynaptic VB calcium currents might explain deleterious cocaine effects on sensory thalamic nuclei. These results suggest that cocaine and MPH produced distinct presynaptic alterations on GABAergic transmission. © 2012 International Society for Neurochemistry.


Documento: Artículo
Título:Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei
Autor:Goitia, B.; Raineri, M.; González, L.E.; Rozas, J.L.; Garcia-Rill, E.; Bisagno, V.; Urbano, F.J.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET-UBA), Ciudad Universitaria, Intendente Guiraldes 2670, pabellõn 2, piso 2, (C1428BGA)-Buenos Aires, Argentina
Instituto de Investigaciones Farmacolõgicas (ININFA- CONICET-UBA-), Junín 956, piso 5, C1113-Buenos Aires, Argentina
Center for Translational Neuroscience, University of Arkansas for Medical Sciences, Little Rock AR, United States
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, (C1428ADN) Buenos Aires, Argentina
Palabras clave:cocaine; GABAergic transmission; methylphenidate; T-type calcium channels; thalamic reticular nucleus; ventrobasal thalamic nucleus; 4 aminobutyric acid; calcium channel T type; calcium ion; cocaine; glutamate decarboxylase 67; methylphenidate; 4 aminobutyric acid release; animal cell; animal experiment; animal model; animal tissue; article; calcium current; controlled study; facilitation; GABAergic transmission; locomotion; male; mouse; nerve cell inhibition; nerve projection; nonhuman; postsynaptic membrane; priority journal; thalamus reticular nucleus; thalamus ventral nucleus; Western blotting; Animals; Blotting, Western; Central Nervous System Stimulants; Cocaine; gamma-Aminobutyric Acid; Male; Methylphenidate; Mice; Mice, Inbred C57BL; Patch-Clamp Techniques; Synaptic Transmission; Thalamic Nuclei; Mus
Página de inicio:602
Página de fin:612
Título revista:Journal of Neurochemistry
Título revista abreviado:J. Neurochem.
CAS:4 aminobutyric acid, 28805-76-7, 56-12-2; calcium ion, 14127-61-8; cocaine, 50-36-2, 53-21-4, 5937-29-1; methylphenidate, 113-45-1, 298-59-9; Central Nervous System Stimulants; Cocaine, 50-36-2; Methylphenidate, 113-45-1; gamma-Aminobutyric Acid, 56-12-2


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---------- APA ----------
Goitia, B., Raineri, M., González, L.E., Rozas, J.L., Garcia-Rill, E., Bisagno, V. & Urbano, F.J. (2013) . Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei. Journal of Neurochemistry, 124(5), 602-612.
---------- CHICAGO ----------
Goitia, B., Raineri, M., González, L.E., Rozas, J.L., Garcia-Rill, E., Bisagno, V., et al. "Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei" . Journal of Neurochemistry 124, no. 5 (2013) : 602-612.
---------- MLA ----------
Goitia, B., Raineri, M., González, L.E., Rozas, J.L., Garcia-Rill, E., Bisagno, V., et al. "Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei" . Journal of Neurochemistry, vol. 124, no. 5, 2013, pp. 602-612.
---------- VANCOUVER ----------
Goitia, B., Raineri, M., González, L.E., Rozas, J.L., Garcia-Rill, E., Bisagno, V., et al. Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei. J. Neurochem. 2013;124(5):602-612.