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Abstract:

Several studies have suggested that esterase-2 (EST-2) may be the target of natural selection in the cactophilic fly Drosophila buzzatii. In this work, we analyzed nucleotide variation in a fragment of α-esterase5 (αE5), the gene encoding EST-2, in original (Argentinian) and colonized (Australian) populations of D. buzzatii and in its sibling D. koepferae. Estimates of nucleotide heterozygosity in D. buzzatii were similar in Australia and Argentina, although we detected a loss of singletons in colonized populations, suggesting a moderate founder effect. Interspecific comparisons revealed that D. buzzatii was more polymorphic for nonsynonymous variation, whereas D. koepferae was more variable for synonymous and noncoding sites. The two major chromosomal arrangements (2st and 2j) in D. buzzatii displayed similar levels of nucleotide variation, whereas 2jz3 was monomorphic. The sequenced region allowed the discrimination of a greater number of EST-2 protein variants in the Australian sample than in the Argentinean sample. In D. koepferae, nucleotide variation in αE5 does not depart from neutral expectations, although tests of population structure were significant for silent variation. In contrast, D. buzzatii has probably undergone a recent population expansion in its South American range. In addition, the McDonald and Kreitman test revealed an excess of nonsynonymous polymorphism in both original and colonized populations of this species. © 2006 Springer Science+Business Media, Inc.

Registro:

Documento: Artículo
Título:Molecular population genetics of the α-esterase5 gene locus in original and colonized populations of Drosophila buzzatii and its sibling Drosophila koepferae
Autor:Piccinali, R.V.; Mascord, L.J.; Barker, J.S.F.; Oakeshott, J.G.; Hasson, E.
Filiación:Departamento de Ecología, Genética Y Evolución, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina
School of Rural Science and Agriculture, Faculty of the Sciences, University of New England, Armidale, NSW, Australia
Division of Entomology, Commonwealth Scientific and Industrial Research Organisation, Canberra, ACT, Australia
Palabras clave:αE5; Cactophilic Drosophila; Excess of nonsynonymous polymorphism; Founder effect; Population expansion; esterase; article; comparative study; controlled study; DNA polymorphism; Drosophila; gene locus; genetic variability; heterozygosity; intraspecific variation; molecular genetics; nonhuman; nucleotide sequence; population genetics; protein variant; sibling; statistical significance; Animals; Base Sequence; Carboxylic Ester Hydrolases; Chromosome Mapping; DNA Primers; Drosophila; Drosophila Proteins; Genetics, Population; Species Specificity; Variation (Genetics); Drosophila buzzatii; Drosophila koepferae
Año:2007
Volumen:64
Número:2
Página de inicio:158
Página de fin:170
DOI: http://dx.doi.org/10.1007/s00239-005-0224-y
Título revista:Journal of Molecular Evolution
Título revista abreviado:J. Mol. Evol.
ISSN:00222844
CODEN:JMEVA
CAS:esterase, 9013-79-0; Carboxylic Ester Hydrolases, EC 3.1.1.-; DNA Primers; Drosophila Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v64_n2_p158_Piccinali

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Citas:

---------- APA ----------
Piccinali, R.V., Mascord, L.J., Barker, J.S.F., Oakeshott, J.G. & Hasson, E. (2007) . Molecular population genetics of the α-esterase5 gene locus in original and colonized populations of Drosophila buzzatii and its sibling Drosophila koepferae. Journal of Molecular Evolution, 64(2), 158-170.
http://dx.doi.org/10.1007/s00239-005-0224-y
---------- CHICAGO ----------
Piccinali, R.V., Mascord, L.J., Barker, J.S.F., Oakeshott, J.G., Hasson, E. "Molecular population genetics of the α-esterase5 gene locus in original and colonized populations of Drosophila buzzatii and its sibling Drosophila koepferae" . Journal of Molecular Evolution 64, no. 2 (2007) : 158-170.
http://dx.doi.org/10.1007/s00239-005-0224-y
---------- MLA ----------
Piccinali, R.V., Mascord, L.J., Barker, J.S.F., Oakeshott, J.G., Hasson, E. "Molecular population genetics of the α-esterase5 gene locus in original and colonized populations of Drosophila buzzatii and its sibling Drosophila koepferae" . Journal of Molecular Evolution, vol. 64, no. 2, 2007, pp. 158-170.
http://dx.doi.org/10.1007/s00239-005-0224-y
---------- VANCOUVER ----------
Piccinali, R.V., Mascord, L.J., Barker, J.S.F., Oakeshott, J.G., Hasson, E. Molecular population genetics of the α-esterase5 gene locus in original and colonized populations of Drosophila buzzatii and its sibling Drosophila koepferae. J. Mol. Evol. 2007;64(2):158-170.
http://dx.doi.org/10.1007/s00239-005-0224-y