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Abstract:

A family of repetitive DNA elements of approximately 350 bp - Sat350 - that are members of Toxoplasma gondii satellite DNA was further analyzed. Sequence analysis identified at least three distinct repeat types within this family, called types A, B, and C. B repeats were divided into the subtypes B1 and B2. A search for internal repetitions within this family permitted the identification of conserved regions and the design of PCR primers that amplify almost all these repetitive elements. These primers amplified the expected 350-bp repeats and a novel 680-bp repetitive element (Sat680) related to this family. Two additional tandemly repeated high-order structures corresponding to this satellite DNA family were found by searching the Toxoplasma genome database with these sequences. These studies were confirmed by sequence analysis and identified: (1) an arrangement of AB1CB2 350-bp repeats and (2) an arrangement of two 350-bp-like repeats, resulting in a 680-bp monomer. Sequence comparison and phylogenetic analysis indicated that both high-order structures may have originated from the same ancestral 350-bp repeat. PCR amplification, sequence analysis and Southern blot showed that similar high-order structures were also found in the Toxoplasma-sister taxon Neospora caninum. The Toxoplasma genome database (http://ToxoDB.org) permitted the assembly of a contig harboring Sat350 elements at one end and a long nonrepetitive DNA sequence flanking this satellite DNA. The region bordering the Sat350 repeats contained two differentially expressed sequence-related regions and interstitial telomeric sequences.

Registro:

Documento: Artículo
Título:Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence
Autor:Clemente, M.; De Miguel, N.; Lia, V.V.; Matrajt, M.; Angel, S.O.
Filiación:INGEBI, Ciudad de Buenos Aires, Argentina
Lab. de Parasitologia Molecular, UB2, CONICET-UNSAM, Camino Circunvalacion Laguna km. 6, Prov. Buenos Aires, Argentina
Depto. de Ecologia, Genet. y Evol., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, United States
Departamento de Parasitologiá, ANLIS Dr. Carlos G. Malbran, Ciudad de Buenos Aires, Argentina
Palabras clave:EST; Heterochromatin; Neospora caninum; Satellite DNA; Tandem repeat; Toxoplasma gondii; monomer; primer DNA; repetitive DNA; satellite DNA; article; controlled study; correlation analysis; data base; DNA flanking region; DNA sequence; gene amplification; gene expression; gene identification; gene structure; genetic analysis; genetic conservation; genome; molecular evolution; molecular phylogeny; multigene family; Neospora caninum; nonhuman; nucleotide sequence; polymerase chain reaction; protein assembly; sequence analysis; Southern blotting; structure analysis; telomere; Toxoplasma gondii; Animals; Bacteriophages; Cloning, Molecular; Contig Mapping; DNA, Satellite; Evolution, Molecular; Expressed Sequence Tags; Neospora; Phylogeny; Sequence Alignment; Sequence Analysis, DNA; Toxoplasma; Neospora caninum; Protozoa; Toxoplasma; Toxoplasma gondii
Año:2004
Volumen:58
Número:5
Página de inicio:557
Página de fin:567
DOI: http://dx.doi.org/10.1007/s00239-003-2578-3
Título revista:Journal of Molecular Evolution
Título revista abreviado:J. Mol. Evol.
ISSN:00222844
CODEN:JMEVA
CAS:DNA, Satellite
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222844_v58_n5_p557_Clemente

Referencias:

  • Ajzenberg, D., Banuls, A.L., Tybayrenc, M., Darde, M.L., Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups (2002) Int J Parasitol, 32, pp. 27-38
  • Andrades-Miranda, J., Zanchin, N.I., Oliveira, L.F., Langguth, A.R., Mattevi, M.S., (T2AG3)n telomeric sequence hybridization indicating centric fusion rearrangements in the karyotype of the rodent Oryzomys subflavus (2002) Genetica, 114, pp. 11-16
  • Blanco, J.C., Angel, S.O., Serpente, P., Pszenny, V., Maero, E., Garberi, J.C., Cloning of repetitive DNA sequences from Toxoplasma gondii and its usefulness for parasite detection (1992) Am J Trop Med Hyg, 46, pp. 350-357
  • Cabot, E.L., Doshi, P., Wu, M.-L., Wu, C.-I., Population genetics of tandem repeats in centromeric heterochromatin: Unequal crossing over and chromosomal divergence at the responder locus of Drosophila melanogaster (1993) Genetics, 135, pp. 477-487
  • Cafasso, D., Cozzolino, S., De Luca, P., Chinali, G., An unusual satellite DNA from Zamia paucijuga (Cycadales) characterised by two different organisations of the repetitive unit in the plant genome (2003) Gene, 311, pp. 71-79
  • Castagnone Sereno, P., Leroy, H., Semblat, J.-P., Leroy, F., Abad, P., Zijlstra, C., Unusual and strongly structured sequence variation in a complex satellite DNA family from the nematode Meloidogyne chitwoodi (1998) J Mol Evol, 46, pp. 225-233
  • Charlesworth, B., Sniegowski, P., Stephan, W., The evolutionary dynamics of repetitive in eukaryotes (1994) Nature, 371, pp. 215-220
  • Cristina, N., Liaud, M.F., Santoro, F., Oury, B., Ambroise-Thomas, P.A., A family of repeated DNAs sequences in Toxoplasma gondii: Cloning, sequence analysis and use in strain characterization (1991) Exp Parasitol, 73, pp. 73-81
  • Cristina, N., Dard, M.L., Boudin, C., Tavernier, F., Pestre-Alexandre, M., Ambroise-Thomas, P.A., A DNA finger-printing method for individual characterization of Toxoplasma gondii strains: Combination with isoenzymatic characters for determination of linkage groups (1995) Parasitol Res, 81, pp. 32-37
  • Cronn, R.C., Zhao, X., Paterson, A.H., Wendel, J.F., Polymorphism and concerted evolution in a tandemly repeated gene family: 5S ribosomal DNA in diploid and allopolyploid cottons (1996) J Mol Evol, 42, pp. 685-705
  • Csink, A.K., Henikoff, S., Something from nothing: The evolution and utility of satellite repeats (1998) Trends Genet, 14, pp. 200-204
  • Dard, M.L., Biodiversity in Toxoplasma gondii (1996) Curr Top Microbiol Immunol, 219, pp. 27-41
  • Elder, J.F., Turner, B.J., Concerted evolution of repetitive DNA sequences in eukaryotes (1995) Q Rev Biol, 70, pp. 297-320
  • Felsenstein, J., Confidence limits on phylogenies: An approach using the boostrap (1985) Evolution, 39, pp. 783-791
  • Echeverria, P.C., Rojas, P.A., Martin, V., Guarnera, E.A., Pszenny, V., Angel, S.O., Characterization of an interspersed Toxoplasma gondii DNA repeat with potential uses for PCR diagnosis and PCR-RFLP analysis (2000) FEMS Microbiol Lett, 184, pp. 23-27
  • Goldeberg, I.G., Sawhney, H., Pluta, A.F., Wartburton, P.E., Earnshaw, W.C., Surprising deficiency of CENP-B binding sites in African green monkey alpha-satellite DNA: Implication for CENP-B function at centromeres (1996) Mol Cell Biol, 16, pp. 5156-5168
  • Grigg, M.E., Ganatra, J., Boothroyd, J.C., Margolis, T.P., Unusual abundance of atypical strains associated with human ocular toxoplasmosis (2001) J Infect Dis, 184, pp. 633-639
  • Grigg, M.E., Bonnefoy, S., Hehl, A.B., Suzuki, Y., Boothroyd, J.C., Success and virulence in Toxoplasma as the result of sexual recombination between two distinct ancestries (2001) Science, 294, pp. 161-165
  • Henning, W., Henning, I., Stein, H., Repeated sequences in the DNA of Drosophila and their localization in giant chromosomes (1970) Chromosoma, 32, pp. 31-63
  • Hodgall, E., Vuust, J., Lind, P., Petersen, E., Characterization of Toxoplasma gondii isolates using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the noncoding Toxoplasma gondii (TGR)-gene sequences (2000) Int J Parasitol, 30, pp. 853-858
  • Homan, W.L., Vercammen, M., De Braekeleer, J., Verschueren, H., Identification of a 200-300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR (2000) Int J Parasitol, 30, pp. 69-75
  • Howe, D.K., Sibley, L.D., Toxoplasma gondii comprises three clonal lineages: Correlation of parasite genotype with human disease (1995) J Infect Dis, 172, pp. 1561-1566
  • Jeanmougin, F., Thompson, J.D., Gouy, M., Higgins, D.G., Gibson, T.J., Multiple sequence alignment with Clustal X (1998) Trends Biochem Sci, 23, pp. 403-405
  • Jones, J., Flavell, R., The structure, amount and chromosomal localization of defined repeated DNA sequences in species of the genus Secale (1982) Chromosoma, 86, pp. 613-641
  • Kimura, M., A simple method for estimating evolutionary rates of base substitution through comparative studied of nucleotide sequences (1980) J Mol Evol, 16, pp. 111-120
  • Koch, J., Neocentromeres and alpha satellite: A proposed structural code for functional human centromere DNA (2000) Hum Mol Genet, 9, pp. 149-154
  • Liao, D., Concerted evolution: Molecular mechanism and biological implications (1999) Am J Hum Genet, 64, pp. 24-30
  • Literak, I., Rychlik, I., Svobodova, V., Pospisil, Z., Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains (1998) Int J Parasitol, 28, pp. 1367-1374
  • Matrajt, M., Angel, S.O., Pszenny, V., Guarnera, E., Roos, D.S., Garberi, J.C., Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii (1999) Genome, 42, pp. 265-269
  • Mestrovick, N., Plohl, M., Mravinac, B., Ugarkovic, D., Evolution of satellite DNAs from the genus Palorus - Experimental evidence for the "Library" hypotesis (1998) Mol Biol Evol, 15, pp. 1062-1068
  • Muro, Y., Masumoto, H., Yoda, K., Nozaki, N., Ohashi, M., Okazaki, T., Centromere protein B assembles human centromeric alpha-satellite DNA at the 17-bp sequence, CENP-B box (1992) J Cell Biol, 116, pp. 585-596
  • Nagylaki, T., Gene conversion, linkage, and the evolution of repeated genes dispersed among multiple chromosomes (1990) Genetics, 106, pp. 529-548
  • Nijman, I.J., Lenstra, J.A., Mutation and recombination in cattle satellite DNA: A feedback model for the evolution of satellite DNA repeats (2001) J Mol Evol, 52, pp. 361-371
  • Ossorio, P.N., Sibley, L.D., Boothroyd, J.C., Mitochondrial-like DNA sequences flanked by direct and inverted repeats in the nuclear genome of Toxoplasma gondii (1991) J Mol Biol, 222, pp. 525-536
  • Plohl, M., Cornurdella, L., Characterization of a complex satellite DNA in the mollusc Donax trunculus: Analysis of sequence variations and divergence (1996) Gene, 169, pp. 157-164
  • Reinhart, B.J., Bartel, D.P., Small RNAs correspond to centromere heterochromatic repeats (2002) Science, 297, p. 1831
  • Rocco, L., Costagliola, D., Stingo, V., (TTAGGG)n telomeric sequence in Selachian chromosomes (2001) Heredity, 87, pp. 583-588
  • Rose, M.R., Doolittle, W., Molecular biological mechanisms of speciation (1983) Science, 220, pp. 157-162
  • Ruiz-Herrera, A., Garcia, F., Azzalin, C., Giulotto, E., Egozcue, J., Ponsa, M., Garcia, M., Distribution of intrachromosomal telomeric sequences (ITS) on Macaca fascicularis (Primates) chromosomes and their implications for chromosome evolution (2002) Hum Genet, 110, pp. 578-586
  • Sambrook, J., Fritsh, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
  • Schimenti, J.C., Mice and role of unequal recombination in gene-family evolution (1999) Am J Hum Genet, 64, pp. 24-30
  • Schlotterer, C., Tautz, D., Chromosomal homogeneity of Drosophila ribosomal DNA arrays suggests intrachromosomal exchanges drive concerted evolution (1994) Curr Biol, 4, pp. 777-783
  • Shirley, M.W., The genome of Eimeria tenella: Further studies on its molecular organisation (1994) Parasitol Res, 80, pp. 366-373
  • Sibley, L.D., Boothroyd, J.C., Construction of a molecular karyotype for Toxoplasma gondii (1992) Mol Biochem Parasitol, 51, pp. 291-300
  • Su, C.L., Evans, D., Cole, R.H., Kissinger, J.C., Ajioka, J.W., Sibley, L.D., Enhanced oral transmission acquired through recent recombination led to widespread expansion of Toxoplasma (2003) Science, 299, pp. 414-416
  • Terry, R.S., Smith, J.E., Duncanson, P., Hide, G., MGE-PCR: A novel approach to the analysis of Toxoplasma gondii strain differentiation using mobile genetic elements (2001) Int J Parasitol, 31, pp. 155-161
  • Volpe, T.A., Kidner, C., Hall, I.M., Teng, G., Grewal, S.I.S., Martienssen, R.A., Regulation of heterochromatic silencing and histone H3 lysine-9 meethylation by RNAi (2002) Science, 297, pp. 1833-1837
  • Warburton, P.E., Waye, J.S., Willard, H.F., Nonrandom localization of recombination events in human alpha satellite repeat unit variants: Implications for higher-order structural characteristics within centromeric heterochromatin (1993) Mol Cell Biol, 13, pp. 6520-6529
  • Waye, J.S., Willard, H.F., Structure, organization, and sequence of alpha satellite DNA from human chromosome 17: Evidence fro evolution by unequal crossing-over and ancestral pentamer repeat shared with the human X chromosome (1986) Mol Cell Biol, 6, pp. 3156-3165
  • Waye, J.S., England, S.B., Willard, H.F., Genomic organization of alpha satellite DNA on human chromosome 7: Evidence for two distinct alphoid domains on a single chromosome (1987) Mol Cell Biol, 7, pp. 349-356
  • Wichman, H.A., Payne, C.T., Ryder, O.A., Hamilton, M.J., Maltibe, M., Baker, R.J., Genomic distribution of heterochromatin sequences in equids: Implications to rapid chromosomal evolution (1991) J Hered, 82, pp. 369-377

Citas:

---------- APA ----------
Clemente, M., De Miguel, N., Lia, V.V., Matrajt, M. & Angel, S.O. (2004) . Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence. Journal of Molecular Evolution, 58(5), 557-567.
http://dx.doi.org/10.1007/s00239-003-2578-3
---------- CHICAGO ----------
Clemente, M., De Miguel, N., Lia, V.V., Matrajt, M., Angel, S.O. "Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence" . Journal of Molecular Evolution 58, no. 5 (2004) : 557-567.
http://dx.doi.org/10.1007/s00239-003-2578-3
---------- MLA ----------
Clemente, M., De Miguel, N., Lia, V.V., Matrajt, M., Angel, S.O. "Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence" . Journal of Molecular Evolution, vol. 58, no. 5, 2004, pp. 557-567.
http://dx.doi.org/10.1007/s00239-003-2578-3
---------- VANCOUVER ----------
Clemente, M., De Miguel, N., Lia, V.V., Matrajt, M., Angel, S.O. Structure analysis of two Toxoplasma gondii and Neospora caninum satellite DNA families and evolution of their common monomeric sequence. J. Mol. Evol. 2004;58(5):557-567.
http://dx.doi.org/10.1007/s00239-003-2578-3