Artículo

Waisman, A.; Vazquez Echegaray, C.; Solari, C.; Cosentino, M.S.; Martyn, I.; Deglincerti, A.; Ozair, M.Z.; Ruzo, A.; Barañao, L.; Miriuka, S.; Brivanlou, A.; Guberman, A. "Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit" (2017) Journal of Molecular Biology. 429(18):2802-2815
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Abstract:

The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the ground state of pluripotency. We show that initial transcriptional changes in this transition do not require passage through mitosis and that conversion to primed pluripotency is linked to lineage priming in the G1 phase. Importantly, we demonstrate that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response. Our data suggest an important role for DNA replication during mouse embryonic stem cell differentiation, which could shed light on why pluripotent cells are only receptive to differentiation signals during G1, that is, before the S phase. © 2017 Elsevier Ltd

Registro:

Documento: Artículo
Título:Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
Autor:Waisman, A.; Vazquez Echegaray, C.; Solari, C.; Cosentino, M.S.; Martyn, I.; Deglincerti, A.; Ozair, M.Z.; Ruzo, A.; Barañao, L.; Miriuka, S.; Brivanlou, A.; Guberman, A.
Filiación:Universidad de Buenos Aires, Laboratorio de Regulación Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET—Universidad de Buenos Aires, Instituto de Química Biológica (IQUIBICEN), Buenos Aires, Argentina
Laboratory of Stem Cell Biology and Molecular Embryology, The Rockefeller University, New York, NY 10065, United States
CONICET—Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Laboratorio de Investigación de Aplicación a Neurociencias (LIAN), Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:cell division; DNA replication; mouse embryonic stem cells introduction; primitive ectoderm-like cells; DNA methyltransferase 3A; kruppel like factor 4; octamer transcription factor 6; protein p53; transcription factor NANOG; transcription factor Otx2; animal cell; Article; cell cycle; cell cycle G1 phase; cell cycle regulation; cell cycle S phase; cell differentiation; cell division; controlled study; DNA replication; mitosis; mouse; mouse embryonic stem cell; nonhuman; pluripotent stem cell; priority journal; stem cell; animal; cell differentiation; genetic transcription; physiology; pluripotent stem cell; Animals; Cell Differentiation; Cell Division; DNA Replication; Mice; Mouse Embryonic Stem Cells; Pluripotent Stem Cells; Transcription, Genetic
Año:2017
Volumen:429
Número:18
Página de inicio:2802
Página de fin:2815
DOI: http://dx.doi.org/10.1016/j.jmb.2017.06.020
Título revista:Journal of Molecular Biology
Título revista abreviado:J. Mol. Biol.
ISSN:00222836
CODEN:JMOBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v429_n18_p2802_Waisman

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Citas:

---------- APA ----------
Waisman, A., Vazquez Echegaray, C., Solari, C., Cosentino, M.S., Martyn, I., Deglincerti, A., Ozair, M.Z.,..., Guberman, A. (2017) . Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit. Journal of Molecular Biology, 429(18), 2802-2815.
http://dx.doi.org/10.1016/j.jmb.2017.06.020
---------- CHICAGO ----------
Waisman, A., Vazquez Echegaray, C., Solari, C., Cosentino, M.S., Martyn, I., Deglincerti, A., et al. "Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit" . Journal of Molecular Biology 429, no. 18 (2017) : 2802-2815.
http://dx.doi.org/10.1016/j.jmb.2017.06.020
---------- MLA ----------
Waisman, A., Vazquez Echegaray, C., Solari, C., Cosentino, M.S., Martyn, I., Deglincerti, A., et al. "Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit" . Journal of Molecular Biology, vol. 429, no. 18, 2017, pp. 2802-2815.
http://dx.doi.org/10.1016/j.jmb.2017.06.020
---------- VANCOUVER ----------
Waisman, A., Vazquez Echegaray, C., Solari, C., Cosentino, M.S., Martyn, I., Deglincerti, A., et al. Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit. J. Mol. Biol. 2017;429(18):2802-2815.
http://dx.doi.org/10.1016/j.jmb.2017.06.020