Abstract:
The DNA-binding mechanism of the dimeric C-terminal domain of the papillomavirus E2 protein with its specific DNA target was investigated and shown to proceed through two parallel pathways. A sequential multi-step reaction is initiated by the diffusion-controlled formation of an encounter complex, with no evidence of base sequence discrimination capacity. Following a substantial conformational rearrangement of the protein, a solvent exclusion step leading to the formation of a final protein-DNA complex was identified. This last step involves the largest burial of surface area from the interface and involves the consolidation of the direct readout of the DNA bases. Double-jump stopped-flow experiments allowed us to characterize the sequence of events and demonstrated that a fast-formed consolidated complex can take place through a parallel route. We present the simplest model for the overall mechanism with a description of all the intermediate species in energetic terms. © 2003 Elsevier Ltd. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | A protein-DNA binding mechanism proceeds through multi-state or two-state parallel pathways |
Autor: | Ferreiro, D.U.; De Prat-Gay, G. |
Filiación: | Instituto Leloir, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, 1405 Buenos Aires, Argentina
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Palabras clave: | DNA-binding; HPV-E2; Protein-DNA; Recognition; Stopped-flow; DNA base; virus protein; article; DNA binding; nucleotide sequence; Papilloma virus; priority journal; protein analysis; protein DNA binding; protein domain; Papillomavirus |
Año: | 2003
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Volumen: | 331
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Número: | 1
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Página de inicio: | 89
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Página de fin: | 99
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DOI: |
http://dx.doi.org/10.1016/S0022-2836(03)00720-4 |
Título revista: | Journal of Molecular Biology
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Título revista abreviado: | J. Mol. Biol.
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ISSN: | 00222836
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CODEN: | JMOBA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222836_v331_n1_p89_Ferreiro |
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Citas:
---------- APA ----------
Ferreiro, D.U. & De Prat-Gay, G.
(2003)
. A protein-DNA binding mechanism proceeds through multi-state or two-state parallel pathways. Journal of Molecular Biology, 331(1), 89-99.
http://dx.doi.org/10.1016/S0022-2836(03)00720-4---------- CHICAGO ----------
Ferreiro, D.U., De Prat-Gay, G.
"A protein-DNA binding mechanism proceeds through multi-state or two-state parallel pathways"
. Journal of Molecular Biology 331, no. 1
(2003) : 89-99.
http://dx.doi.org/10.1016/S0022-2836(03)00720-4---------- MLA ----------
Ferreiro, D.U., De Prat-Gay, G.
"A protein-DNA binding mechanism proceeds through multi-state or two-state parallel pathways"
. Journal of Molecular Biology, vol. 331, no. 1, 2003, pp. 89-99.
http://dx.doi.org/10.1016/S0022-2836(03)00720-4---------- VANCOUVER ----------
Ferreiro, D.U., De Prat-Gay, G. A protein-DNA binding mechanism proceeds through multi-state or two-state parallel pathways. J. Mol. Biol. 2003;331(1):89-99.
http://dx.doi.org/10.1016/S0022-2836(03)00720-4