Abstract:
Improving the excitation conditions in two-photon fluorescent lithography reduces the size of the fabricated structures to nanometre scales. We demonstrate that a precise control of the illumination profile and the scanning speed of the laser beam is enough to decrease the photo-polymerization volume of resins by orders of magnitude. This work also shows experimental evidence of surface effects that yield a different polymerization intensity threshold compared with bulk. Such phenomenon enables to perform a non-linear optical nanolithography in a simple way, allowing fast-prototyping procedures. We present a detailed study of the polymer growth process using fluorescence and atomic force microscopy. © 2008 The Authors.
Registro:
Documento: |
Artículo
|
Título: | Fluorescent two-photon nanolithography |
Autor: | Kunik, D.; Ludueña, S.J.; Costantino, S.; Martínez, O.E. |
Filiación: | Departamento de Física, FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina Centro de Microscopías Avanzadas, FCEN, Universidad de Buenos Aires, Buenos Aires, Argentina Department of Physics, Montreal Neurological Institute, McGill University, QC, Canada
|
Palabras clave: | Lithography; Nanolithography; Two-photon absorption; polymer; article; atomic force microscopy; fluorescence; image reconstruction; nanotechnology; photon; polymerization; priority journal |
Año: | 2008
|
Volumen: | 229
|
Número: | 3
|
Página de inicio: | 540
|
Página de fin: | 544
|
DOI: |
http://dx.doi.org/10.1111/j.1365-2818.2008.01940.x |
Título revista: | Journal of Microscopy
|
Título revista abreviado: | J. Microsc.
|
ISSN: | 00222720
|
CODEN: | JMICA
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222720_v229_n3_p540_Kunik |
Referencias:
- Axelrod, D., Total internal reflection fluorescence microscopy in cell biology (2001) Traffic, 2 (11), pp. 764-774
- Betzig, E., Patterson, G.H., Sougrat, R., Al, E., Imaging intracellular fluorescent proteins at nanometer resolution (2006) Science, 313 (5793), pp. 1642-1645
- Betzig, E., Trautman, J.K., Near-field optics - Microscopy, spectroscopy, and surface modification beyond the diffraction limit (1992) Science, 257 (5067), pp. 189-195. , &
- Costantino, S., Heinze, K.G., Martinez, O.E., De Koninck, P., Wiseman, P.W., Two-photon fluorescent microlithography for live-cell imaging (2005) Microsc. Res. Tech., 68 (5), pp. 272-276. , &
- Cumpston, B.H., Ananthavel, S.P., Barlow, S., Al, E., Two-photon polymerization initiators for three-dimensional optical data storage and microfabrication (1999) Nature, 398 (6722), pp. 51-54
- Galajda, P., Ormos, P., Complex micromachines produced and driven by light (2001) Appl. Phys. Lett., 78 (2), pp. 249-251. , &
- Gustafsson, M.G.L., Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy (2000) J. Microsc. (Oxford), 198, pp. 82-87
- Hofmann, M., Eggeling, C., Jakobs, S., Hell, S.W., Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins (2005) Proc. Natl. Acad. Sci. U. S. A., 102 (49), pp. 17565-17569. , &
- Kawata, S., Sun, H.B., Tanaka, T., Takada, K., Finer features for functional microdevices - Micromachines can be created with higher resolution using two-photon absorption (2001) Nature, 412 (6848), pp. 697-698. , &
- Maruo, S., Nakamura, O., Kawata, S., Three-dimensional microfabrication with two-photon-absorbed photopolymerization (1997) Opt. Lett., 22 (2), pp. 132-134. , &
- Schmid, H., Biebuyck, A., Michel, B., Martien, O.J.F., Piller, N.B., Ligth-couplilng masks: An alternative lensless approach to high-resolution optical contanct lithography (1998) J. Vac. Sci. Technol. B., 16, pp. 3422-3425. , &
- Sun, H.B., Tanaka, T., Takada, K., Kawata, S., Two-photon photopolymerization and diagnosis of three-dimensional microstructures containing fluorescent dyes (2001) Appl. Phys. Lett., 79 (10), pp. 1411-1413. , &
- Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R., Hell, S.W., STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis (2006) Nature, 440 (7086), pp. 935-939. , &
Citas:
---------- APA ----------
Kunik, D., Ludueña, S.J., Costantino, S. & Martínez, O.E.
(2008)
. Fluorescent two-photon nanolithography. Journal of Microscopy, 229(3), 540-544.
http://dx.doi.org/10.1111/j.1365-2818.2008.01940.x---------- CHICAGO ----------
Kunik, D., Ludueña, S.J., Costantino, S., Martínez, O.E.
"Fluorescent two-photon nanolithography"
. Journal of Microscopy 229, no. 3
(2008) : 540-544.
http://dx.doi.org/10.1111/j.1365-2818.2008.01940.x---------- MLA ----------
Kunik, D., Ludueña, S.J., Costantino, S., Martínez, O.E.
"Fluorescent two-photon nanolithography"
. Journal of Microscopy, vol. 229, no. 3, 2008, pp. 540-544.
http://dx.doi.org/10.1111/j.1365-2818.2008.01940.x---------- VANCOUVER ----------
Kunik, D., Ludueña, S.J., Costantino, S., Martínez, O.E. Fluorescent two-photon nanolithography. J. Microsc. 2008;229(3):540-544.
http://dx.doi.org/10.1111/j.1365-2818.2008.01940.x