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Abstract:

Molecular dynamics simulations of lipid bilayers in aqueous systems reveal how an applied electric field stabilizes the reorganization of the water–membrane interface into water-filled, membrane-spanning, conductive pores with a symmetric, toroidal geometry. The pore formation process and the resulting symmetric structures are consistent with other mathematical approaches such as continuum models formulated to describe the electroporation process. Some experimental data suggest, however, that the shape of lipid electropores in living cell membranes may be asymmetric. We describe here the axially asymmetric pores that form when mechanical constraints are applied to selected phospholipid atoms. Electropore formation proceeds even with severe constraints in place, but pore shape and pore formation time are affected. Since lateral and transverse movement of phospholipids may be restricted in cell membranes by covalent attachments to or non-covalent associations with other components of the membrane or to membrane-proximate intracellular or extracellular biomolecular assemblies, these lipid-constrained molecular models point the way to more realistic representations of cell membranes in electric fields. © 2017, Springer Science+Business Media, LLC, part of Springer Nature.

Registro:

Documento: Artículo
Título:Electropore Formation in Mechanically Constrained Phospholipid Bilayers
Autor:Fernández, M.L.; Risk, M.R.; Vernier, P.T.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Física, Buenos Aires, Argentina
CONICET - Universidad de Buenos Aires, Instituto de Física del Plasma (INFIP), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Instituto Tecnológico de Buenos Aires (ITBA), Buenos Aires, Argentina
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States
Palabras clave:Electroporation; Molecular dynamics; Phospholipid bilayer; Position constraints; 2 oleoyl 1 palmitoylphosphatidylcholine; Article; cell membrane; channel gating; covalent bond; electric field; electroporation; extracellular matrix; mechanical constraint; molecular dynamics; molecular model; particle size; phospholipid bilayer; protein assembly; simulation
Año:2018
Volumen:251
Número:2
Página de inicio:237
Página de fin:245
DOI: http://dx.doi.org/10.1007/s00232-017-0002-y
Título revista:Journal of Membrane Biology
Título revista abreviado:J. Membr. Biol.
ISSN:00222631
CODEN:JMBBB
CAS:2 oleoyl 1 palmitoylphosphatidylcholine, 6753-55-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222631_v251_n2_p237_Fernandez

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Citas:

---------- APA ----------
Fernández, M.L., Risk, M.R. & Vernier, P.T. (2018) . Electropore Formation in Mechanically Constrained Phospholipid Bilayers. Journal of Membrane Biology, 251(2), 237-245.
http://dx.doi.org/10.1007/s00232-017-0002-y
---------- CHICAGO ----------
Fernández, M.L., Risk, M.R., Vernier, P.T. "Electropore Formation in Mechanically Constrained Phospholipid Bilayers" . Journal of Membrane Biology 251, no. 2 (2018) : 237-245.
http://dx.doi.org/10.1007/s00232-017-0002-y
---------- MLA ----------
Fernández, M.L., Risk, M.R., Vernier, P.T. "Electropore Formation in Mechanically Constrained Phospholipid Bilayers" . Journal of Membrane Biology, vol. 251, no. 2, 2018, pp. 237-245.
http://dx.doi.org/10.1007/s00232-017-0002-y
---------- VANCOUVER ----------
Fernández, M.L., Risk, M.R., Vernier, P.T. Electropore Formation in Mechanically Constrained Phospholipid Bilayers. J. Membr. Biol. 2018;251(2):237-245.
http://dx.doi.org/10.1007/s00232-017-0002-y