Navegar

Colección

Datos Estadísticas

Artículo

Tranchida, M.C.; Riccillo, P.M.; Rodriguero, M.S.; García, J.J.; Micieli, M.V. "Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae" (2012) Journal of Invertebrate Pathology. 109(1):143-147
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

A survey of drainage ditches in suburban areas of La Plata, Buenos Aires province, Argentina for pathogens of Culex pipiens larvae was conducted from 2003 to 2006. C. pipiens larvae of opaque, white color were found in several of those field collections. When the white larvae were dissected and observed by phase-contrast microscopy in wet-mount preparations, the presence of bacteria, located in the hemocoel, was recorded. Laboratory experiments were performed to elucidate the pathway for transmission of this pathogen. Although approaches involving traditional culturing had failed to reveal the identity of the new microorganism present, molecular techniques to identify the pathogen in the studies reported here were successful. The partial sequence of the 16S-rRNA gene constitutes a powerful tool for the detection of new isolates from the hemocoele of C. pipiens larvae. These bacteria were characterized as belonging to the genus Novispirillum. In spite of the genus's wide distribution in different aquatic environments, information related to the parasitic relationship of Novispirillum spp. to aquatic insects is scarce, and this association has not been described in other mosquito species. This report constitutes the first documentation of Novispirillum spp. as a pathogen for mosquito larvae. © 2011 Elsevier Inc.

Registro:

Documento: Artículo
Título:Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae
Autor:Tranchida, M.C.; Riccillo, P.M.; Rodriguero, M.S.; García, J.J.; Micieli, M.V.
Filiación:Centro de Estudios Parasitológicos y Vectores (CEPAVE-CONICET-CCT-La Plata-UNLP), 2 # 584, La Plata. Cp 1900, Bs As, Argentina
Instituto de Botánica C. Spegazzinni, FCNyM, UNLP, 53 # 477, La Plata. Cp 1900, Bs As, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires Pabellón II, Ciudad Universitaria, Av. Intendente Güiraldes y Av. Costanera Norte s/n, 1428, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:16S-ribosomal-RNA genetic sequence; Bacterial hemocoele; Culex pipiens; Mosquito; bacterial RNA; RNA 16S; Alphaproteobacteria; animal; Argentina; article; classification; Culex; cytology; environmental monitoring; genetics; hemolymph; isolation and purification; larva; methodology; microbiology; sequence analysis; ultrastructure; Alphaproteobacteria; Animals; Argentina; Culex; Environmental Monitoring; Hemolymph; Larva; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Analysis, RNA; Water Microbiology; Alphaproteobacteria; Bacteria (microorganisms); Culex pipiens; Culicidae; Diptera; Hexapoda
Año:2012
Volumen:109
Número:1
Página de inicio:143
Página de fin:147
DOI: http://dx.doi.org/10.1016/j.jip.2011.10.007
Título revista:Journal of Invertebrate Pathology
Título revista abreviado:J. Invertebr. Pathol.
ISSN:00222011
CODEN:JIVPA
CAS:RNA, Bacterial; RNA, Ribosomal, 16S
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00222011_v109_n1_p143_Tranchida

Referencias:

  • Aguilar, M.O., López, M.V., Ricillo, P.M., The diversity of rhizobia nodulating beans in North-west Argentina as a source of more efficient inoculant strains (2001) J. Biotechnol., 91, pp. 181-188
  • Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs (1997) Nucleic Acids Res., 25 (17), pp. 3389-3402
  • Becnel, J.J., Andreadis, G.T., Microsporidia in insect (1999) The microsporidia and microsporidiosis, pp. 447-501. , Asm. Press, Whashington, Wittner, Weiss (Eds.)
  • Becnel, J.J., Floore, T.G., (2007), In: Floore, T.G. (Ed.), Biorrational Control of Mosquitoes. J. Am. Mosq. Control Asoc. 23 (2), 1-2 (Bull. No. 7); Becnel, J., White, S.E., Mosquito pathogenic viruses - The last 20 years (2007), Floore, T.G. (Ed.), Biorational Control of Mosquitoes. J. Am. Mosq. Control Assoc. 23 (2), 36-49 (Bull No 7); Campos, R.E., Maciá, A., García, J.J., Fluctuaciones estacionales de culícidos (Diptera) y sus enemigos naturales en zonas urbanas de los alrededores de La Plata, provincia de Buenos Aires (1993) Neotrópica, 39, pp. 55-66
  • Darsie, R.F., Mosquitoes of Argentina Part I. Keys for identification of adult females and fourth stages larvae in English and Spanish (Diptera: Culicidae) (1985) Mosq. Syst., 17, pp. 153-253
  • Dellapé, M.E., Marti, G.A., Tranchida, M.C., García, J.J., First record of Aedes aegypti (L) (Diptera: Culicidae) infected by the paracite Ascogregarina culicis (Ross) (Apicomplexa: Lecudinidae) in Argentina (2005) Entomología y Vectores., 12 (1), pp. 111-115
  • Federici, B.A., Park, H.W., Bideschi, D.K., Wirth, M.C., Johson, J.J., Sakano, Y., Tang, M., Developing recombinant bacteria for control of mosquito larvae (2007), pp. 164-175. , Biorational control of mosquitoes. Floore, T.G. (Ed.), Am. Mosq. Control Assoc., Inc. 23 (2) No 7; Gillespie, J.P., Kanost, M.R., Trenczek, T., Biological mediators of insect immunity (1997) Ann. Rev. Entomol., 42, pp. 611-643
  • Kerwin, J.L., Oomycetes: Lagenidium giganteum (2007) J. Am. Mosq. Control Assoc., pp. 50-58. , Floore, T.G. (Ed.), Biorational control of mosquitoes. Inc. 23 (2) No 7
  • Lane, J., Neotropical Culicidae (1953) Faculdade de Saúde Pública da Universidade de São Paulo, 2, pp. 633-729
  • Mogi, M., Insect and other invertebrate predators (2007) Biorational control of mosquitoes., pp. 93-109. , Floore, T.G. (Ed.). Am. Mosq. Control Assoc, Inc. 23 (2) No 7
  • Nei, M., Kumar, S., (2000) Molecular evolution and phylogenetics, , Oxford University Press, Oxford/New York
  • Platzer, G.E., Mermithid nematodes. (2007) Biorational control of mosquitoes, pp. 58-64. , Floore, T.G. (Ed.), Am. Mosq. Control Assoc., Inc. 23 (2) No 7
  • Pot, B., Gillis, M., Genus III. Aquaspirillum (2005) Bergeýs Manual of Systematic Bacteriology, pp. 801-823. , Springer, USA, G.M. Garrity (Ed.)
  • Stackebrandt, E., Frederiksen, W., Garrity, G.M., Grimont, P.A.D., Kampfer, P., Maiden, M.C.J., Nesme, X., Whitman, W.B., Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology (2002) Int. J. Syst. Evol. Microbiol., 52, pp. 1043-1047
  • Tainai, K., Wago, H., Yamakawa, M., In vitro phagocytosis of Escherichia coli and release of lipopolysaccharide by adhering hemocytes of the silkworm, Bombyx mori (1997) Biochem. Biophys. Res. Commun., 231 (2), pp. 623-627
  • Tamura, K., Dudley, J., Nei, M., Kumar, S., MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 (2007) Mol. Biol. Evol., 24, pp. 1596-1599
  • Thiery, I., Frachon, E., Identification, isolation, culture and preservation of entomopathogenic bacteria (1997) Manual of techniques in insect pathology, pp. 55-90. , Academic Press, L.A. Lacey (Ed.)
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice (1994) Nucleic Acids Res., 22, pp. 4673-4680
  • Tranchida, M.C., Pelizza, S.A., Bisaro, V., Beltrán, C., García, J.J., Micieli, M.V., Use of the neotropical fish Cnesterodom decemmaculatus for long-term control of Culex pipiens L. in Argentina (2010) Biol. Control., 53 (2), pp. 183-187
  • Tranchida, M.C., Riccillo, P.M., Micieli, M.V., García, J.J., Rodriguero, M.S., Isolation and Identification of Lysinibacillus sphaericus from infected Culex pipiens larvae (Diptera. Culicidae) (2010) Annals Microbiol., 61 (3), pp. 575-584
  • Weir, T., Park, S., Vivanco, J., Biochemical and physiological mechanisms mediated allelochemicals (2004) Plant Biol., 7, pp. 472-479
  • Weisburg, W., Barns, S., Pelletier, D., Lane, J., 16S Ribosomal DNA amplification for phylogenetic study (1991) J. Bacteriol., 173 (2), pp. 697-703
  • Yoon, J.-H., Kang, S.-J., Park, S., Lee, S.-Y., Oh, T.K., Reclassification of Aquaspirillum itersonii and Aquaspirillum peregrinum as Novispirillum itersonii gen. nov., comb. nov. and Insolitispirillum peregrinum gen. nov., comb. nov (2007) Int. J. Syst. Evol. Microbiol., 57, pp. 2830-2835

Citas:

---------- APA ----------
Tranchida, M.C., Riccillo, P.M., Rodriguero, M.S., García, J.J. & Micieli, M.V. (2012) . Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae. Journal of Invertebrate Pathology, 109(1), 143-147.
http://dx.doi.org/10.1016/j.jip.2011.10.007
---------- CHICAGO ----------
Tranchida, M.C., Riccillo, P.M., Rodriguero, M.S., García, J.J., Micieli, M.V. "Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae" . Journal of Invertebrate Pathology 109, no. 1 (2012) : 143-147.
http://dx.doi.org/10.1016/j.jip.2011.10.007
---------- MLA ----------
Tranchida, M.C., Riccillo, P.M., Rodriguero, M.S., García, J.J., Micieli, M.V. "Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae" . Journal of Invertebrate Pathology, vol. 109, no. 1, 2012, pp. 143-147.
http://dx.doi.org/10.1016/j.jip.2011.10.007
---------- VANCOUVER ----------
Tranchida, M.C., Riccillo, P.M., Rodriguero, M.S., García, J.J., Micieli, M.V. Isolation and identification of α-proteobacteria from Culex pipiens (Diptera Culicidae) larvae. J. Invertebr. Pathol. 2012;109(1):143-147.
http://dx.doi.org/10.1016/j.jip.2011.10.007