Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Molecular genetic markers can be used to identify quantitative trait loci (QTL) for thermal resistance and this has allowed characterization of a major QTL for knockdown resistance to high temperature in Drosophila melanogaster. The QTL showed trade-off associations with cold resistance under laboratory conditions. However, assays of thermal tolerance conducted in the laboratory may not necessarily reflect performance at varying temperatures in the field. Here we tested if lines with different genotypes in this QTL show different thermal performance under high and low temperatures in the field using a release recapture assay. We found that lines carrying the QTL genotype for high thermal tolerance were significantly better at locating resources in the field releases under hot temperatures while the QTL line carrying the contrasting genotype were superior at cold temperatures. Further, we studied copulatory success between the different QTL genotypes at different temperatures. We found higher copulatory success in males of the high tolerance QTL genotype under hot temperature conditions, while there was no difference in females at cold temperatures. The results allow relating components of field fitness at different environmental temperatures with genotypic variation in a QTL for thermal tolerance. © 2011 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster
Autor:Loeschcke, V.; Kristensen, T.N.; Norry, F.M.
Filiación:Department of Biological Sciences, Aarhus University, Ny Munkegade 114-116, Building 1540, DK-8000, Aarhus C, Denmark
Department of Genetics and Biotechnology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C-1428-EHA) Buenos Aires, Argentina
Palabras clave:Copulatory success; Fitness in the wild; Genetic variation; Heat and cold resistance; Semi-natural conditions; Stress resistance; bioassay; copulation; fitness; fly; genetic marker; genetic variation; genotype; laboratory method; life history trait; quantitative analysis; temperature tolerance; trade-off; animal; article; copulation; Drosophila melanogaster; female; genetics; genotype; heat; male; quantitative trait locus; Animals; Copulation; Drosophila melanogaster; Female; Genotype; Hot Temperature; Male; Quantitative Trait Loci; Drosophila melanogaster
Año:2011
Volumen:57
Número:9
Página de inicio:1227
Página de fin:1231
DOI: http://dx.doi.org/10.1016/j.jinsphys.2011.05.013
Título revista:Journal of Insect Physiology
Título revista abreviado:J. Insect Physiol.
ISSN:00221910
CODEN:JIPHA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v57_n9_p1227_Loeschcke

Referencias:

  • Barker, J.S.F., East, P.D., Evidence for selection following perturbation of allozyme frequencies in a natural population of Drosophila (1980) Nature, 284, pp. 166-168
  • Bubliy, O.A., Loeschcke, V., Correlated responses to selection for stress resistance and longevity in a laboratory population of D. melanogaster (2005) Journal of Evolutionary Biology, 18, pp. 789-803
  • David, R.J., Gibert, P., Pla, E., Petavy, G., Karan, D., Moreteau, B., Cold stress tolerance in Drosophila: analysis of chill coma recovery in D (1998) Melanogster. Journal of Thermal Biology, 23, pp. 291-299
  • Feder, M.E., Roberts, S.P., Bordelon, A.C., Molecular thermal telemetry of free-ranging adult D. melanogaster (2000) Oecologia, 123, pp. 460-465
  • Hoffmann, A.A., Anderson, A., Hallas, R., Opposing clines for high and low temperature resistance in D. melanogaster (2002) Ecology Letters, 5, pp. 614-618
  • Hoffmann, A.A., Ó Donnell, S., Heritable variation in the attraction of D. melanogaster to fruit in the field (1992) Biological Journal of the Linnean Society, 47, pp. 147-159
  • Hoffmann, A.A., Loeschcke, V., Are fitness effects of density mediated by body size? Evidence from Drosophila field releases (2006) Evolutionary Ecology Research, 8, pp. 813-828
  • Hoffmann, A.A., Ratna, E., Sgro, C.M., Barton, M., Blacket, M., Hallas, R., De Garis, S., Weeks, A.R., Antagonistic selection between adult thorax and wing size in field released D. melanogaster independent of thermal conditions (2007) Journal of Evolutionary Biology, 20, pp. 2219-2227
  • Huey, R.B., Crill, W.D., Kingsolver, J.G., Weber, K.E., A method for rapid measurement of heat of cold resistance of small insects (1992) Functional Ecology, 6, pp. 489-494
  • Jaenike, J., Benway, H., Stevens, G., Parasite-induced mortality in mycophagous Drosophila (1995) Ecology, 76, pp. 383-391
  • Kingsolver, J.G., Experimental analyses of wing size, flight, and survival in the western white butterfly (1999) Evolution, 53, pp. 1479-1490
  • Kristensen, T.N., Loeschcke, V., Hoffmann, A.A., Can artificially selected phenotypes influence a component of field fitness? Thermal selection and fly performance under thermal extremes (2007) Proceedings of the Royal Society of London B, 274, pp. 771-778
  • Kristensen, T.N., Hoffmann, A.A., Overgaard, J., Sørensen, J.G., Hallas, R., Loeschcke, V., Costs and benefits of cold acclimation in field released Drosophila (2008) Proceedings of the National Academy of Sciences of the United States of America, 105, pp. 216-221
  • Kristensen, T.N., Loeschcke, V., Hoffmann, A.A., Linking inbreeding effects in captive populations to field performance-field releases with replicated D. melanogaster lines under different temperatures (2008) Conservation Biology, 22, pp. 189-199
  • Leips, J., Mackay, T.F.C., Quantitative trait loci for life span in D. melanogaster: interactions with genetic background and larval density (2000) Genetics, 155, pp. 1773-1778
  • Loeschcke, V., Hoffmann, A.A., Consequences of heat hardening on a field fitness component in Drosophila depend on environmental temperature (2007) American. Naturalist, 169, pp. 175-183
  • McColl, G., Hoffmann, A.A., McKechnie, S.W., Response of two heat shock genes to selection for knockdown heat resistance in D. melanogaster (1996) Genetics, 143, pp. 1615-1627
  • Morgan, T.J., Mackay, T.F.C., Quantitative trait loci for thermotolerance phenotypes in D. melanogaster (2006) Heredity, 96, pp. 232-242
  • Mori, N., Kimura, M.N., Selection for rapid and slow recovery from chill- and heat-coma in D. melanogaster (2008) Biological Journal of the Linnean Society, 95, pp. 72-80
  • Norry, F.M., Dahlgaard, J., Loeschcke, V., Quantitative trait loci affecting knockdown resistance to high temperature in D. melanogaster (2004) Molecular Ecology, 13, pp. 3585-3594
  • Norry, F.M., Gomez, F.H., Loeschcke, V., Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a central region of chromosome 2 in D. melanogaster (2007) Molecular Ecology, 16, pp. 3274-3284
  • Norry, F.M., Scannapieco, A.C., Sambucetti, P., Bertoli, C., Loeschcke, V., QTL for the thermotolerance effect of heat hardening, knockdown resistance to heat and chill-coma recovery in an intercontinental set of recombinant inbred lines of D. melanogaster (2008) Molecular Ecology, 17, pp. 4570-4581
  • Norry, F.M., Larsen, P.F., Liu, Y., Loeschcke, V., Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in D. melanogaster (2009) Journal of Insect Physiology, 55, pp. 1050-1057
  • Overgaard, J., Sørensen, J.G., Rapid thermal adaptation during field temperature variations in D. melanogaster (2008) Cryobiology, 56, pp. 159-162
  • Overgaard, J., Sørensen, J.G., Jensen, L.T., Loeschcke, V., Kristensen, T.N., Field tests reveal genetic variation for performance at low temperatures in D. melanogaster (2010) Functional Ecology, 24, pp. 186-195
  • Rako, L., Blacket, M.J., McKechnie, S.W., Hoffmann, A.A., Candidate genes and thermal phenotypes: identifying ecologically important genetic variation for thermotolerance in the Australian D. melanogaster cline (2007) Molecular Ecology, 16, pp. 2948-2957
  • Rand, D.M., Weinreich, D.M., Lerman, D., Folk, D., Gilchrist, G.W., Three selections are better than one: clinal variation of thermal QTL from independent selection experiments in Drosophila (2010) Evolution, 64, pp. 2921-2934
  • Sambucetti, P., Scannapieco, A.C., Norry, F.M., Direct and correlated responses to artificial selection for high and low knockdown resistance to high temperature in Drosophila buzzatii (2010) Journal of Thermal Biology, 35, pp. 232-238
  • Sarup, P., Sørensen, J.G., Dimitrov, K., Barker, J.S.F., Loeschcke, V., Climatic adaptation of Drosophila buzzatii populations in southeast Australia (2006) Heredity, 96, pp. 479-486
  • Sørensen, J.G., Norry, F.M., Scannapieco, A.C., Loeschcke, V., Altitudinal variation for stress resistance traits and thermal adaptation in adult Drosophila buzzatii from the New World (2005) Journal of Evolutionary Biology, 18, pp. 829-837
  • Vieira, C., Pasyukova, E.G., Zeng, Z.-B., Hackett, J.B., Lyman, R.F., Mackay, T.F.C., Genotype-environment for quantitative trait loci affecting life span in D. melanogaster (2000) Genetics, 154, pp. 213-227

Citas:

---------- APA ----------
Loeschcke, V., Kristensen, T.N. & Norry, F.M. (2011) . Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster. Journal of Insect Physiology, 57(9), 1227-1231.
http://dx.doi.org/10.1016/j.jinsphys.2011.05.013
---------- CHICAGO ----------
Loeschcke, V., Kristensen, T.N., Norry, F.M. "Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster" . Journal of Insect Physiology 57, no. 9 (2011) : 1227-1231.
http://dx.doi.org/10.1016/j.jinsphys.2011.05.013
---------- MLA ----------
Loeschcke, V., Kristensen, T.N., Norry, F.M. "Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster" . Journal of Insect Physiology, vol. 57, no. 9, 2011, pp. 1227-1231.
http://dx.doi.org/10.1016/j.jinsphys.2011.05.013
---------- VANCOUVER ----------
Loeschcke, V., Kristensen, T.N., Norry, F.M. Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster. J. Insect Physiol. 2011;57(9):1227-1231.
http://dx.doi.org/10.1016/j.jinsphys.2011.05.013