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Abstract:

This report shows the biochemical characterization and life cycle-dependent expression of Drosophila melanogaster N-β-alanyldopamine synthase (NBAD-synthase or Ebony protein). This enzyme not only catalyzes the synthesis of NBAD, the main sclerotization and pigmentation precursor of insect brown cuticles, but also plays a role in brain neurotransmitter metabolism. In addition to the epidermis expression our immunodetection experiments show the novel localization of NBAD-synthase in different regions of the adult brain, in the foregut of pharate adult and, surprisingly, in the epidermis of the trachea during embryogenesis. These results demonstrate that NBAD-synthase is a versatile enzyme involved in different, previously unknown, time- and tissue-dependent processes. © 2009 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster
Autor:Pérez, M.M.; Schachter, J.; Berni, J.; Quesada-Allué, L.A.
Filiación:Department of Biological Chemistry, FCEyN, University of Buenos Aires, Patricias Argentinas 435, Buenos Aires 1405, Argentina
Palabras clave:Cuticle; Insect brain; Neurotransmitters; Sclerotization; Tracheal development; DNA binding protein; Drosophila protein; ebony protein, Drosophila; biochemistry; cuticle; enzyme activity; fly; life cycle; metabolism; animal; article; Drosophila melanogaster; enzymology; epidermis; female; gastrointestinal tract; immunohistochemistry; life cycle; metabolism; nervous system; prenatal development; trachea; Animals; DNA-Binding Proteins; Drosophila melanogaster; Drosophila Proteins; Epidermis; Female; Gastrointestinal Tract; Immunohistochemistry; Life Cycle Stages; Nervous System; Trachea; Drosophila melanogaster; Hexapoda
Año:2010
Volumen:56
Número:1
Página de inicio:8
Página de fin:13
DOI: http://dx.doi.org/10.1016/j.jinsphys.2009.08.018
Título revista:Journal of Insect Physiology
Título revista abreviado:J. Insect Physiol.
ISSN:00221910
CODEN:JIPHA
CAS:DNA-Binding Proteins; Drosophila Proteins; ebony protein, Drosophila
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221910_v56_n1_p8_Perez

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Citas:

---------- APA ----------
Pérez, M.M., Schachter, J., Berni, J. & Quesada-Allué, L.A. (2010) . The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster. Journal of Insect Physiology, 56(1), 8-13.
http://dx.doi.org/10.1016/j.jinsphys.2009.08.018
---------- CHICAGO ----------
Pérez, M.M., Schachter, J., Berni, J., Quesada-Allué, L.A. "The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster" . Journal of Insect Physiology 56, no. 1 (2010) : 8-13.
http://dx.doi.org/10.1016/j.jinsphys.2009.08.018
---------- MLA ----------
Pérez, M.M., Schachter, J., Berni, J., Quesada-Allué, L.A. "The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster" . Journal of Insect Physiology, vol. 56, no. 1, 2010, pp. 8-13.
http://dx.doi.org/10.1016/j.jinsphys.2009.08.018
---------- VANCOUVER ----------
Pérez, M.M., Schachter, J., Berni, J., Quesada-Allué, L.A. The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster. J. Insect Physiol. 2010;56(1):8-13.
http://dx.doi.org/10.1016/j.jinsphys.2009.08.018