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Valguarnera, E.; Spera, J.M.; Czibener, C.; Fulgenzi, F.R.; Casabuono, A.C.; Altabe, S.G.; Pasquevich, K.A.; Guaimas, F.; Cassataro, J.; Couto, A.S.; Ugalde, J.E. "RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella" (2018) Journal of Infectious Diseases. 217(8):1257-1266
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Abstract:

Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response. This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities. Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response. We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane. A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation. We show that RomA is involved in the synthesis of LPS, probably coordinating part of the biosynthetic complex in the periplasm. Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype. Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation. © 2017 The Author(s).

Registro:

Documento: Artículo
Título:RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella
Autor:Valguarnera, E.; Spera, J.M.; Czibener, C.; Fulgenzi, F.R.; Casabuono, A.C.; Altabe, S.G.; Pasquevich, K.A.; Guaimas, F.; Cassataro, J.; Couto, A.S.; Ugalde, J.E.
Filiación:Instituto de Investigaciones Biotecnológicas dr. Rodolfo A. Ugalde, IIB-INTECH, CONICET, Universidad Nacional de San Martín, Campus Miguelete, UNSAM, Avenida 25 de Mayo y Francia, San Martín (1650), Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, Departamento de Quimica Organica-Consejo Nacional de Lnvestigaciones Cientificas y Tecnicas, Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biología Molecular y Celular de Rosario (IBR), Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina
Palabras clave:Brucella; inflammation; lipopolysaccharide; lipopolysaccharide; periplasmic protein; RomA protein; unclassified drug; Alphaproteobacteria; animal experiment; animal model; Article; bacterial gene; bacterial outer membrane; bacterial strain; bacterial virulence; bacterium adherence; biosynthesis; Brucella abortus; brucellosis; carbohydrate analysis; carbohydrate synthesis; controlled study; homeostasis; inflammation; macromolecule; membrane damage; mouse; mutant; nonhuman; periplasm; phenotype; priority journal; protein carbohydrate interaction; protein determination; protein function
Año:2018
Volumen:217
Número:8
Página de inicio:1257
Página de fin:1266
DOI: http://dx.doi.org/10.1093/infdis/jiy002
Título revista:Journal of Infectious Diseases
Título revista abreviado:J. Infect. Dis.
ISSN:00221899
CODEN:JIDIA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221899_v217_n8_p1257_Valguarnera

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Citas:

---------- APA ----------
Valguarnera, E., Spera, J.M., Czibener, C., Fulgenzi, F.R., Casabuono, A.C., Altabe, S.G., Pasquevich, K.A.,..., Ugalde, J.E. (2018) . RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella. Journal of Infectious Diseases, 217(8), 1257-1266.
http://dx.doi.org/10.1093/infdis/jiy002
---------- CHICAGO ----------
Valguarnera, E., Spera, J.M., Czibener, C., Fulgenzi, F.R., Casabuono, A.C., Altabe, S.G., et al. "RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella" . Journal of Infectious Diseases 217, no. 8 (2018) : 1257-1266.
http://dx.doi.org/10.1093/infdis/jiy002
---------- MLA ----------
Valguarnera, E., Spera, J.M., Czibener, C., Fulgenzi, F.R., Casabuono, A.C., Altabe, S.G., et al. "RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella" . Journal of Infectious Diseases, vol. 217, no. 8, 2018, pp. 1257-1266.
http://dx.doi.org/10.1093/infdis/jiy002
---------- VANCOUVER ----------
Valguarnera, E., Spera, J.M., Czibener, C., Fulgenzi, F.R., Casabuono, A.C., Altabe, S.G., et al. RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella. J. Infect. Dis. 2018;217(8):1257-1266.
http://dx.doi.org/10.1093/infdis/jiy002