Artículo

Poncini, C.V.; Ilarregui, J.M.; Batalla, E.I.; Engels, S.; Cerliani, J.P.; Cucher, M.A.; Van Kooyk, Y.; González-Cappa, S.M.; Rabinovich, G.A. "Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms" (2015) Journal of Immunology. 195(7):3311-3324
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Abstract:

Galectin-1 (Gal-1), an endogenous glycan-binding protein, is widely distributed at sites of inflammation and microbial invasion. Despite considerable progress regarding the immunoregulatory activity of this lectin, the role of endogenous Gal-1 during acute parasite infections is uncertain. In this study, we show that Gal-1 functions as a negative regulator to limit host-protective immunity following intradermal infection with Trypanosoma cruzi. Concomitant with the upregulation of immune inhibitory mediators, including IL-10, TGF-β1, IDO, and programmed death ligand 2, T. cruzi infection induced an early increase of Gal-1 expression in vivo. Compared to their wild-type (WT) counterpart, Gal-1-deficient (Lgals1-/-) mice exhibited reduced mortality and lower parasite load in muscle tissue. Resistance of Lgals1-/- mice to T. cruzi infection was associated with a failure in the activation of Gal-1-driven tolerogenic circuits, otherwise orchestrated by WT dendritic cells, leading to secondary dysfunction in the induction of CD4+CD25+Foxp3+ regulatory T cells. This effect was accompanied by an increased number of CD8+ T cells and higher frequency of IFN-γ-producing CD4+ T cells in muscle tissues and draining lymph nodes as well as reduced parasite burden in heart and hindlimb skeletal muscle. Moreover, dendritic cells lacking Gal-1 interrupted the Gal-1-mediated tolerogenic circuit and reinforced T cell-dependent anti-parasite immunity when adoptively transferred into WT mice. Thus, endogenous Gal-1 may influence T. cruzi infection by fueling tolerogenic circuits that hinder anti-parasite immunity.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms
Autor:Poncini, C.V.; Ilarregui, J.M.; Batalla, E.I.; Engels, S.; Cerliani, J.P.; Cucher, M.A.; Van Kooyk, Y.; González-Cappa, S.M.; Rabinovich, G.A.
Filiación:Instituto de Investigaciones en Microbiología y Parasitología Médicas, Facultad de Medicina, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Paraguay 2155,Piso 13, Buenos Aires, C1121, Argentina
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, C1428, Argentina
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, 1081 HV, Netherlands
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1428, Argentina
Palabras clave:CD4 antigen; galectin 1; interleukin 10; interleukin 17; interleukin 2 receptor alpha; transcription factor FOXP3; transforming growth factor beta1; galectin 1; gamma interferon; IDO1 protein, mouse; IL10 protein, mouse; indoleamine 2,3 dioxygenase; interleukin 10; Pdcd1lg2 protein, mouse; programmed death 1 ligand 2; transforming growth factor beta1; analysis of variance; animal cell; animal experiment; animal model; animal tissue; Article; CD8+ T lymphocyte; cell activation; cell differentiation; cell survival; cell viability; Chagas disease; confocal microscopy; controlled study; cytokine production; dendritic cell; disease predisposition; glycosylation; homeostasis; immunoblotting; immunogenicity; immunomodulation; lymphocyte proliferation; male; mononuclear cell; mouse; nonhuman; pathogen load; priority journal; protein analysis; protein binding; protein function; quantitative analysis; real time polymerase chain reaction; T lymphocyte; Th1 cell; Th17 cell; upregulation; animal; Bagg albino mouse; biosynthesis; C3H mouse; Chagas disease; cytology; dendritic cell; genetics; immunology; knockout mouse; lymph node; metabolism; mortality; parasite load; parasitology; regulatory T lymphocyte; Trypanosoma cruzi; Animals; CD8-Positive T-Lymphocytes; Chagas Disease; Dendritic Cells; Galectin 1; Indoleamine-Pyrrole 2,3,-Dioxygenase; Interferon-gamma; Interleukin-10; Lymph Nodes; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Knockout; Parasite Load; Programmed Cell Death 1 Ligand 2 Protein; T-Lymphocytes, Regulatory; Transforming Growth Factor beta1; Trypanosoma cruzi
Año:2015
Volumen:195
Número:7
Página de inicio:3311
Página de fin:3324
DOI: http://dx.doi.org/10.4049/jimmunol.1403019
Título revista:Journal of Immunology
Título revista abreviado:J. Immunol.
ISSN:00221767
CODEN:JOIMA
CAS:galectin 1, 258495-34-0; gamma interferon, 82115-62-6; indoleamine 2,3 dioxygenase; Galectin 1; IDO1 protein, mouse; IL10 protein, mouse; Indoleamine-Pyrrole 2,3,-Dioxygenase; Interferon-gamma; Interleukin-10; Pdcd1lg2 protein, mouse; Programmed Cell Death 1 Ligand 2 Protein; Transforming Growth Factor beta1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v195_n7_p3311_Poncini

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Citas:

---------- APA ----------
Poncini, C.V., Ilarregui, J.M., Batalla, E.I., Engels, S., Cerliani, J.P., Cucher, M.A., Van Kooyk, Y.,..., Rabinovich, G.A. (2015) . Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms. Journal of Immunology, 195(7), 3311-3324.
http://dx.doi.org/10.4049/jimmunol.1403019
---------- CHICAGO ----------
Poncini, C.V., Ilarregui, J.M., Batalla, E.I., Engels, S., Cerliani, J.P., Cucher, M.A., et al. "Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms" . Journal of Immunology 195, no. 7 (2015) : 3311-3324.
http://dx.doi.org/10.4049/jimmunol.1403019
---------- MLA ----------
Poncini, C.V., Ilarregui, J.M., Batalla, E.I., Engels, S., Cerliani, J.P., Cucher, M.A., et al. "Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms" . Journal of Immunology, vol. 195, no. 7, 2015, pp. 3311-3324.
http://dx.doi.org/10.4049/jimmunol.1403019
---------- VANCOUVER ----------
Poncini, C.V., Ilarregui, J.M., Batalla, E.I., Engels, S., Cerliani, J.P., Cucher, M.A., et al. Trypanosoma cruzi infection imparts a regulatory program in dendritic cells and T cells via galectin-1-dependent mechanisms. J. Immunol. 2015;195(7):3311-3324.
http://dx.doi.org/10.4049/jimmunol.1403019