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Abstract:

We previously demonstrated that extracellular bacterial DNA activates neutrophils through a CpG- and TLR9-independent mechanism. Biofilms are microbial communities enclosed in a polymeric matrix that play a critical role in the pathogenesis of many infectious diseases. Because extracellular DNA is a key component of biofilms of different bacterial species, the aim of this study was to determine whether it plays a role in the ability of biofilms to induce human neutrophil activation. We found that degradation of matrix extracellular DNA with DNase I markedly reduced the capacity of Pseudomonas aeruginosa biofilms to induce the release of the neutrophil proinflammatory cytokines IL-8 and IL-1β (>75%); reduced the upregulation of neutrophil activation markers CD18, CD11b, and CD66b (p < 0.001); reduced the number of bacteria phagocytosed per neutrophil contacting the biofilm; and reduced the production of neutrophil extracellular traps. Consistent with these findings, we found that biofilms formed by the lasI rhlI P. aeruginosa mutant strain, exhibiting a very low content of matrix extracellular DNA, displayed a lower capacity to stimulate the release of proinflammatory cytokines by neutrophils, which was not decreased further by DNase I treatment. Together, our findings support that matrix extracellular DNA is a major proinflammatory component of P. aeruginosa biofilms. Copyright © 2010 by The American Association of Immunologists, Inc.

Registro:

Documento: Artículo
Título:Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms
Autor:Fuxman Bass, J.I.; Russo, D.M.; Gabelloni, M.L.; Geffner, J.R.; Giordano, M.; Catalano, M.; Zorreguieta, Á.; Trevani, A.S.
Filiación:Departamento de Inmunología, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Fundación Instituto Leloir, Instituto de Investigaciones Bioquímicas, Buenos Aires CONICET, Buenos Aires, Argentina
Palabras clave:CD11b antigen; CD18 antigen; CD66b antigen; deoxyribonuclease I; DNA; interleukin 1beta; interleukin 8; lymphocyte antigen; unclassified drug; bacterial DNA; cytokine; article; bacterial strain; biofilm; controlled study; cytokine release; degradation; extracellular matrix; human; leukocyte activation; nonhuman; phagocytosis; priority journal; Pseudomonas aeruginosa; upregulation; biosynthesis; chemistry; confocal microscopy; extracellular fluid; growth, development and aging; immunology; leukocyte activation; metabolism; microbiology; neutrophil; physiology; Biofilms; Cytokines; DNA, Bacterial; Extracellular Fluid; Humans; Microscopy, Confocal; Neutrophil Activation; Neutrophils; Pseudomonas aeruginosa
Año:2010
Volumen:184
Número:11
Página de inicio:6386
Página de fin:6395
DOI: http://dx.doi.org/10.4049/jimmunol.0901640
Título revista:Journal of Immunology
Título revista abreviado:J. Immunol.
ISSN:00221767
CODEN:JOIMA
CAS:DNA, 9007-49-2; deoxyribonuclease I, 9003-98-9; interleukin 8, 114308-91-7; Cytokines; DNA, Bacterial
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00221767_v184_n11_p6386_FuxmanBass.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221767_v184_n11_p6386_FuxmanBass

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Citas:

---------- APA ----------
Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., Zorreguieta, Á.,..., Trevani, A.S. (2010) . Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms. Journal of Immunology, 184(11), 6386-6395.
http://dx.doi.org/10.4049/jimmunol.0901640
---------- CHICAGO ----------
Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., et al. "Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms" . Journal of Immunology 184, no. 11 (2010) : 6386-6395.
http://dx.doi.org/10.4049/jimmunol.0901640
---------- MLA ----------
Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., et al. "Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms" . Journal of Immunology, vol. 184, no. 11, 2010, pp. 6386-6395.
http://dx.doi.org/10.4049/jimmunol.0901640
---------- VANCOUVER ----------
Fuxman Bass, J.I., Russo, D.M., Gabelloni, M.L., Geffner, J.R., Giordano, M., Catalano, M., et al. Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms. J. Immunol. 2010;184(11):6386-6395.
http://dx.doi.org/10.4049/jimmunol.0901640