Perone, M.J.; Bertera, S.; Shufesky, W.J.; Divito, S.J.; Montecalvo, A.; Mathers, A.R.; Larregina, A.T.; Pang, M.; Seth, N.; Wucherpfennig, K.W.; Trucco, M.; Baum, L.G.; Morelli, A.E. "Suppression of autoimmune diabetes by soluble galectin-1" (2009) Journal of Immunology. 182(5):2641-2653
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Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease that targets the β-cells of the pancreas. We investigated the ability of soluble galectin-1 (gal-1), an endogenous lectin that promotes T cell apoptosis, to down-regulate the T cell response that destroys the pancreatic β-cells. We demonstrated that in nonobese diabetic (NOD) mice, gal-1 therapy reduces significantly the amount of Th1 cells, augments the number of T cells secreting IL-4 or IL-10 specific for islet cell Ag, and causes peripheral deletion of β-cell-reactive T cells. Administration of gal-1 prevented the onset of hyperglycemia in NOD mice at early and subclinical stages of T1D. Preventive gal-1 therapy shifted the composition of the insulitis into an infiltrate that did not invade the islets and that contained a significantly reduced number of Th1 cells and a higher percentage of CD4+ T cells with content of IL-4, IL-5, or IL-10. The beneficial effects of gal-1 correlated with the ability of the lectin to trigger apoptosis of the T cell subsets that cause β-cell damage while sparing naive T cells, Th2 lymphocytes, and regulatory T cells in NOD mice. Importantly, gal-1 reversed β-cell autoimmunity and hyperglycemia in NOD mice with ongoing T1D. Because gal-1 therapy did not cause major side effects or β-cell toxicity in NOD mice, the use of gal-1 to control β-cell autoimmunity represents a novel alternative for treatment of subclinical or ongoing T1D. Copyright © 2009 by The American Association of Immunologists, Inc.


Documento: Artículo
Título:Suppression of autoimmune diabetes by soluble galectin-1
Autor:Perone, M.J.; Bertera, S.; Shufesky, W.J.; Divito, S.J.; Montecalvo, A.; Mathers, A.R.; Larregina, A.T.; Pang, M.; Seth, N.; Wucherpfennig, K.W.; Trucco, M.; Baum, L.G.; Morelli, A.E.
Filiación:Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, United States
Department of Dermatology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, United States
Department of Immunology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, United States
Department of Pediatrics, Division of Immunogenetics, University of Pittsburgh, Pittsburgh, PA 15213, United States
Department of Pathology, Jonsson Comprehensive Cancer Center, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, United States
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, United States
Laboratorio de Fisiologia y Biologia Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:galectin 1; interleukin 10; interleukin 4; interleukin 5; antidiabetic agent; galectin 1; animal cell; animal experiment; animal model; animal tissue; article; autoimmune disease; CD4+ T lymphocyte; controlled study; diabetes mellitus; down regulation; helper cell; hyperglycemia; insulitis; mouse; nonhuman; priority journal; regulatory T lymphocyte; T lymphocyte; animal; autoimmune disease; Bagg albino mouse; female; human; immunology; insulin dependent diabetes mellitus; intraperitoneal drug administration; metabolism; mouse mutant; nonobese diabetic mouse; pancreas islet beta cell; pathology; physiology; transgenic mouse; Animals; Autoimmune Diseases; Diabetes Mellitus, Type 1; Female; Galectin 1; Humans; Hypoglycemic Agents; Injections, Intraperitoneal; Insulin-Secreting Cells; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Mice, SCID; Mice, Transgenic
Página de inicio:2641
Página de fin:2653
Título revista:Journal of Immunology
Título revista abreviado:J. Immunol.
CAS:galectin 1, 258495-34-0; Galectin 1; Hypoglycemic Agents


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---------- APA ----------
Perone, M.J., Bertera, S., Shufesky, W.J., Divito, S.J., Montecalvo, A., Mathers, A.R., Larregina, A.T.,..., Morelli, A.E. (2009) . Suppression of autoimmune diabetes by soluble galectin-1. Journal of Immunology, 182(5), 2641-2653.
---------- CHICAGO ----------
Perone, M.J., Bertera, S., Shufesky, W.J., Divito, S.J., Montecalvo, A., Mathers, A.R., et al. "Suppression of autoimmune diabetes by soluble galectin-1" . Journal of Immunology 182, no. 5 (2009) : 2641-2653.
---------- MLA ----------
Perone, M.J., Bertera, S., Shufesky, W.J., Divito, S.J., Montecalvo, A., Mathers, A.R., et al. "Suppression of autoimmune diabetes by soluble galectin-1" . Journal of Immunology, vol. 182, no. 5, 2009, pp. 2641-2653.
---------- VANCOUVER ----------
Perone, M.J., Bertera, S., Shufesky, W.J., Divito, S.J., Montecalvo, A., Mathers, A.R., et al. Suppression of autoimmune diabetes by soluble galectin-1. J. Immunol. 2009;182(5):2641-2653.