Artículo

Nieves, M.; De Oliveira, E.H.C.; Amaral, P.J.S.; Nagamachi, C.Y.; Pieczarka, J.C.; Mühlmann, M.C.; Mudry, M.D. "Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons" (2011) Journal of Genetics. 90(1):111-117
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Abstract:

The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences. © 2011 Indian Academy of Sciences.

Registro:

Documento: Artículo
Título:Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
Autor:Nieves, M.; De Oliveira, E.H.C.; Amaral, P.J.S.; Nagamachi, C.Y.; Pieczarka, J.C.; Mühlmann, M.C.; Mudry, M.D.
Filiación:Grupo de Investigación en Biología Evolutiva (GIBE), Laboratorio 46, 4 piso, Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Pabellón II - Ciudad Universitaria, Intendente Güiraldes 2160 - C1428EGA, Ciudad Autónoma de Buenos Aires, Argentina
Laboratório de Citogenética, Departamento de Genética, Centro de Ciências Biológicas, Rua Augusto Corrêa, Universidade Federal do Pará, No. 1 Campus Básico 66075-110, Belém, PA, Brazil
Laboratorio de Citogenética Molecular, Instituto Nacional de Tecnología Industrial, Universidad de San Martín, Av. Gral. Paz 5445, Edif. 23, C1650 San Martín, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Cientificas y Técnicas, Av. Rivadavia, 1917 C1033AAJ Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Cebus; chromosome microdissection; FISH; heterochromatin; Platyrrhini; primate evolution; animal; article; Cebus; chromosome; chromosome banding pattern; comparative study; fluorescence in situ hybridization; genetics; heterochromatin; karyotyping; microdissection; nucleotide repeat; Animals; Cebus; Chromosome Banding; Chromosomes; Heterochromatin; In Situ Hybridization, Fluorescence; Karyotyping; Microdissection; Repetitive Sequences, Nucleic Acid; Callitrichidae; Cebidae; Cebus; Cebus albifrons; Cebus apella; Cebus kaapori; Cebus libidinosus; Cebus olivaceus; Cebus xanthosternos; Platyrrhini; Primates
Año:2011
Volumen:90
Número:1
Página de inicio:111
Página de fin:117
DOI: http://dx.doi.org/10.1007/s12041-011-0047-0
Título revista:Journal of Genetics
Título revista abreviado:J. Genet.
ISSN:00221333
CODEN:JOGNA
CAS:Heterochromatin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221333_v90_n1_p111_Nieves

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Citas:

---------- APA ----------
Nieves, M., De Oliveira, E.H.C., Amaral, P.J.S., Nagamachi, C.Y., Pieczarka, J.C., Mühlmann, M.C. & Mudry, M.D. (2011) . Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons. Journal of Genetics, 90(1), 111-117.
http://dx.doi.org/10.1007/s12041-011-0047-0
---------- CHICAGO ----------
Nieves, M., De Oliveira, E.H.C., Amaral, P.J.S., Nagamachi, C.Y., Pieczarka, J.C., Mühlmann, M.C., et al. "Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons" . Journal of Genetics 90, no. 1 (2011) : 111-117.
http://dx.doi.org/10.1007/s12041-011-0047-0
---------- MLA ----------
Nieves, M., De Oliveira, E.H.C., Amaral, P.J.S., Nagamachi, C.Y., Pieczarka, J.C., Mühlmann, M.C., et al. "Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons" . Journal of Genetics, vol. 90, no. 1, 2011, pp. 111-117.
http://dx.doi.org/10.1007/s12041-011-0047-0
---------- VANCOUVER ----------
Nieves, M., De Oliveira, E.H.C., Amaral, P.J.S., Nagamachi, C.Y., Pieczarka, J.C., Mühlmann, M.C., et al. Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons. J. Genet. 2011;90(1):111-117.
http://dx.doi.org/10.1007/s12041-011-0047-0