Artículo

Rodríguez, M.E.; Brunetti, J.E.; Wachsman, M.B.; Scolaro, L.A.; Castilla, V. "Raf/MEK/ERK pathway activation is required for Junín virus replication" (2014) Journal of General Virology. 95(PART 4):799-805
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Abstract:

In the present work we investigated the importance of the Raf/MEK/ERK signalling pathway in the multiplication of the arenavirus Junín (JUNV) in monkey and human cell cultures. We established that JUNV induces a biphasic activation of ERK and we proved that a specific inhibitor of the ERK pathway, U0126, impairs viral replication. Furthermore, U0126 exerted inhibitory action against the arenaviruses Tacaribe and Pichinde. Moreover, treatment with known ERK activators such as phorbol 12-myristate 13-acetate and serum increased viral yields whereas ERK silencing by small interfering RNAs caused the inhibition of viral multiplication. Therefore, activation of the Raf/MEK/ ERK signalling pathway is required to ensure efficient JUNV replication and may constitute a host target for the development of novel effective therapeutic strategies to deal with arenavirus infections. © 2014 SGM.

Registro:

Documento: Artículo
Título:Raf/MEK/ERK pathway activation is required for Junín virus replication
Autor:Rodríguez, M.E.; Brunetti, J.E.; Wachsman, M.B.; Scolaro, L.A.; Castilla, V.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene; mitogen activated protein kinase; phorbol 13 acetate 12 myristate; small interfering RNA; animal cell; Arenavirus; arenavirus infection; Argentine hemorrhagic fever; article; cell culture; cell strain U937; controlled study; enzyme activation; enzyme phosphorylation; human; human cell; Junin virus; nonhuman; priority journal; signal transduction; Vero cell; virus replication; Animals; Cell Line; Extracellular Signal-Regulated MAP Kinases; Gene Knockdown Techniques; Haplorhini; Host-Pathogen Interactions; Humans; Junin virus; MAP Kinase Signaling System; Virus Replication
Año:2014
Volumen:95
Número:PART 4
Página de inicio:799
Página de fin:805
DOI: http://dx.doi.org/10.1099/vir.0.061242-0
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
ISSN:00221317
CODEN:JGVIA
CAS:1,4 diamino 1,4 bis(2 aminophenylthio) 2,3 dicyanobutadiene, 109511-58-2; mitogen activated protein kinase, 142243-02-5; phorbol 13 acetate 12 myristate, 16561-29-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v95_nPART4_p799_Rodriguez

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Citas:

---------- APA ----------
Rodríguez, M.E., Brunetti, J.E., Wachsman, M.B., Scolaro, L.A. & Castilla, V. (2014) . Raf/MEK/ERK pathway activation is required for Junín virus replication. Journal of General Virology, 95(PART 4), 799-805.
http://dx.doi.org/10.1099/vir.0.061242-0
---------- CHICAGO ----------
Rodríguez, M.E., Brunetti, J.E., Wachsman, M.B., Scolaro, L.A., Castilla, V. "Raf/MEK/ERK pathway activation is required for Junín virus replication" . Journal of General Virology 95, no. PART 4 (2014) : 799-805.
http://dx.doi.org/10.1099/vir.0.061242-0
---------- MLA ----------
Rodríguez, M.E., Brunetti, J.E., Wachsman, M.B., Scolaro, L.A., Castilla, V. "Raf/MEK/ERK pathway activation is required for Junín virus replication" . Journal of General Virology, vol. 95, no. PART 4, 2014, pp. 799-805.
http://dx.doi.org/10.1099/vir.0.061242-0
---------- VANCOUVER ----------
Rodríguez, M.E., Brunetti, J.E., Wachsman, M.B., Scolaro, L.A., Castilla, V. Raf/MEK/ERK pathway activation is required for Junín virus replication. J. Gen. Virol. 2014;95(PART 4):799-805.
http://dx.doi.org/10.1099/vir.0.061242-0