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Abstract:

Heterogeneous nuclear ribonucleoproteins A and B (hnRNPs A/B), cellular RNA-binding proteins that participate in splicing, trafficking, translation and turnover of mRNAs, have been implicated in the life cycles of several cytoplasmic RNA viruses. Here, we demonstrate that silencing of hnRNPs A1 and A2 significantly reduces the replication of the arenavirus Juni{dotless} ́n virus (JUNV), the aetiological agent of Argentine haemorrhagic fever. While acute JUNV infection did not modify total levels of expression of hnRNPs A/B in comparison with uninfected cells, non-cytopathic persistent infection exhibited low levels of these cell proteins. Furthermore, acutely infected cells showed a cytoplasmic relocalization of overexpressed hnRNP A1, probably related to the involvement of this protein in virus replicative cycle. This cytoplasmic accumulation was also observed in cells expressing viral nucleoprotein (N), and co-immunoprecipitation studies revealed the interaction between hnRNP A1 and N protein. By contrast, a predominantly nuclear distribution of overexpressed hnRNP A1 was found during persistent infection, even in the presence of endogenous or overexpressed N protein, indicating a differential modulation of nucleo-cytoplasmic trafficking in acute and persistent JUNV infections. © 2011 SGM.

Registro:

Documento: Artículo
Título:Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B
Autor:Maeto, C.A.; Knott, M.E.; Linero, F.N.; Ellenberg, P.C.; Scolaro, L.A.; Castilla, V.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro Nacional de Referencia para el SIDA, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Piso 11, Buenos Aires, Argentina
Infectious Diseases Unit, Alfred Hospital, Level 2, Burnet Building, 85 Commercial Road, Melbourne, VIC 3004, Australia
Palabras clave:cell protein; ribonucleoprotein; ribonucleoprotein A1; ribonucleoprotein A2; ribonucleoprotein B; small interfering RNA; unclassified drug; virus nucleoprotein; Argentine hemorrhagic fever; article; cellular distribution; controlled study; gene overexpression; immunoprecipitation; Junin virus; nonhuman; nucleocytoplasmic transport; persistent infection; priority journal; protein expression; protein protein interaction; Vero cell; virus cell interaction; virus replication; Active Transport, Cell Nucleus; Animals; Cercopithecus aethiops; Heterogeneous-Nuclear Ribonucleoprotein Group A-B; Host-Pathogen Interactions; Immunoprecipitation; Junin virus; Protein Binding; RNA, Viral; Vero Cells; Viral Proteins; Virus Replication; Arenavirus; Junin virus; RNA viruses
Año:2011
Volumen:92
Número:9
Página de inicio:2181
Página de fin:2190
DOI: http://dx.doi.org/10.1099/vir.0.030163-0
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
ISSN:00221317
CODEN:JGVIA
CAS:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; RNA, Viral; Viral Proteins; hnRNP A1
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00221317_v92_n9_p2181_Maeto.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n9_p2181_Maeto

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Citas:

---------- APA ----------
Maeto, C.A., Knott, M.E., Linero, F.N., Ellenberg, P.C., Scolaro, L.A. & Castilla, V. (2011) . Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. Journal of General Virology, 92(9), 2181-2190.
http://dx.doi.org/10.1099/vir.0.030163-0
---------- CHICAGO ----------
Maeto, C.A., Knott, M.E., Linero, F.N., Ellenberg, P.C., Scolaro, L.A., Castilla, V. "Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B" . Journal of General Virology 92, no. 9 (2011) : 2181-2190.
http://dx.doi.org/10.1099/vir.0.030163-0
---------- MLA ----------
Maeto, C.A., Knott, M.E., Linero, F.N., Ellenberg, P.C., Scolaro, L.A., Castilla, V. "Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B" . Journal of General Virology, vol. 92, no. 9, 2011, pp. 2181-2190.
http://dx.doi.org/10.1099/vir.0.030163-0
---------- VANCOUVER ----------
Maeto, C.A., Knott, M.E., Linero, F.N., Ellenberg, P.C., Scolaro, L.A., Castilla, V. Differential effect of acute and persistent Junín virus infections on the nucleo-cytoplasmic trafficking and expression of heterogeneous nuclear ribonucleoproteins type A and B. J. Gen. Virol. 2011;92(9):2181-2190.
http://dx.doi.org/10.1099/vir.0.030163-0