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The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.


Documento: Artículo
Título:Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
Autor:Talarico, L.B.; Noseda, M.D.; Ducatti, D.R.B.; Duarte, M.E.R.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil
Fundación Infant, Gavilán 94, 1406 Buenos Aires, Argentina
Palabras clave:carrageenan; heparan sulfate; animal cell; antiviral activity; article; cell proliferation; controlled study; dengue; Dengue virus; insect cell; mammal cell; mosquito; nonhuman; priority journal; protein synthesis; Vero cell; virus inhibition; Animals; Carrageenan; Cell Line; Cercopithecus aethiops; Culicidae; Dengue; Dengue Virus; Vero Cells; Virus Replication; Dengue virus; Mammalia
Página de inicio:1332
Página de fin:1342
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; heparan sulfate, 9050-30-0; Carrageenan, 9000-07-1


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---------- APA ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R. & Damonte, E.B. (2011) . Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan. Journal of General Virology, 92(6), 1332-1342.
---------- CHICAGO ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. "Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan" . Journal of General Virology 92, no. 6 (2011) : 1332-1342.
---------- MLA ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. "Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan" . Journal of General Virology, vol. 92, no. 6, 2011, pp. 1332-1342.
---------- VANCOUVER ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan. J. Gen. Virol. 2011;92(6):1332-1342.