Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.

Registro:

Documento: Artículo
Título:Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
Autor:Talarico, L.B.; Noseda, M.D.; Ducatti, D.R.B.; Duarte, M.E.R.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil
Fundación Infant, Gavilán 94, 1406 Buenos Aires, Argentina
Palabras clave:carrageenan; heparan sulfate; animal cell; antiviral activity; article; cell proliferation; controlled study; dengue; Dengue virus; insect cell; mammal cell; mosquito; nonhuman; priority journal; protein synthesis; Vero cell; virus inhibition; Animals; Carrageenan; Cell Line; Cercopithecus aethiops; Culicidae; Dengue; Dengue Virus; Vero Cells; Virus Replication; Dengue virus; Mammalia
Año:2011
Volumen:92
Número:6
Página de inicio:1332
Página de fin:1342
DOI: http://dx.doi.org/10.1099/vir.0.028522-0
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
ISSN:00221317
CODEN:JGVIA
CAS:carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; heparan sulfate, 9050-30-0; Carrageenan, 9000-07-1
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00221317_v92_n6_p1332_Talarico.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v92_n6_p1332_Talarico

Referencias:

  • Avirutnan, P., Zhang, L., Punyadee, N., Manuyakorn, A., Puttikhunt, C., Kasinrerk, W., Malasit, P., Diamond, M.S., Secreted NS1 of dengue virus attaches to the surface of cells via interactions with heparan sulfate and chondroitin sulfate (2007) E. PLoS Pathog, e183, p. 3
  • Baeuerle, P.A., Huttner, W.B., Chlorate-a potent inhibitor of protein sulfation in intact cells (1986) Biochem Biophys Res Commun, 141, pp. 870-877
  • Barth, H., Schnober, E.K., Zhang, F., Linhardt, R.J., Depla, E., Boson, B., Cosset, F.L., Baumert, T.F., Viral and cellular determinants of the hepatitis C virus envelope-heparan sulfate interaction (2006) J Virol, 80, pp. 10579-10590
  • Bishop, J.R., Schuksz, M., Esko, J.D., Heparan sulphate proteoglycans fine-tune mammalian physiology (2007) Nature, 446, pp. 1030-1037
  • Buck, C.B., Thompson, C.D., Roberts, J.N., Müller, M., Lowy, D.R., Schiller, J.T., Carrageenan is a potent inhibitor of papillomavirus infection (2006) PLoS Pathog, e69, p. 2
  • Chee, H.Y., Abubakar, S., Identification of a 48kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry (2004) Biochem Biophys Res Commun, 320, pp. 11-17
  • Chen, Y., Maguire, T., Hileman, R.E., Fromm, J.R., Esko, J.D., Linhardt, R.J., Marks, R.M., Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate (1997) Nat Med, 3, pp. 866-871
  • Damonte, E.B., Matulewicz, M.C., Cerezo, A.S., Sulfated seaweed polysaccharides as antiviral agents (2004) Curr Med Chem, 11, pp. 2399-2419
  • de Tischer, S.F.P.C., Talarico, L.B., Noseda, M.D., Guimaraes, S.M.P.B., Damonte, E.B., Duarte, M.E.R., Chemical structure and antiviral activity of carrageenans from Meristiella gelidium against herpes simplex and dengue virus (2006) Carbohydr Polym, 63, pp. 459-465
  • Esko, J.D., Selleck, S.B., Order out of chaos: Assembly of ligand binding sites in heparan sulfate (2002) Annu Rev Biochem, 71, pp. 435-471
  • Germi, R., Crance, J.M., Garin, D., Guimet, J., Lortat-Jacob, H., Ruigrok, R.W., Zarski, J.P., Drouet, E., Heparan sulfatemediated binding of infectious dengue virus type 2 and yellow fever virus (2002) Virology, 292, pp. 162-168
  • Gubler, D.J., Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century (2002) Trends Microbiol, 10, pp. 100-103
  • Hidari, K.I., Takahashi, N., Arihara, M., Nagaoka, M., Morita, K., Suzuki, T., Structure and anti-dengue virus activity of sulfated polysaccharide from a marine alga (2008) Biochem Biophys Res Commun, 376, pp. 91-95
  • Hilgard, P., Stockert, R., Heparan sulfate proteoglycans initiate dengue virus infection of hepatocytes (2000) Hepatology, 32, pp. 1069-1077
  • Hung, S.L., Lee, P.L., Chen, H.W., Chen, L.K., Kao, C.L., King, C.C., Analysis of the steps involved in Dengue virus entry into host cells (1999) Virology, 257, pp. 156-167
  • Hung, J.J., Hsieh, M.T., Young, M.J., Kao, C.L., King, C.C., Chang, W., An external loop region of domain III of dengue virus type 2 envelope protein is involved in serotype-specific binding to mosquito but not mammalian cells (2004) J Virol, 78, pp. 378-388
  • Kuadkitkan, A., Wikan, N., Fongsaran, C., Smith, D.R., Identification and characterization of prohibitin as a receptor protein mediating DENV-2 entry into insect cells (2010) Virology, 406, pp. 149-161
  • Lee, E., Pavy, M., Young, N., Freeman, C., Lobigs, M., Antiviral effect of the heparan sulfate mimetic, PI-88, against dengue and encephalitic flaviviruses (2006) Antiviral Res, 69, pp. 31-38
  • Lin, Y.L., Lei, H.Y., Lin, Y.S., Yeh, T.M., Chen, S.H., Liu, H.S., Heparin inhibits dengue-2 virus infection of five human liver cell lines (2002) Antiviral Res, 56, pp. 93-96
  • Marks, R.M., Lu, H., Sundaresan, R., Toida, T., Suzuki, A., Imanari, T., Hernáiz, M.J., Linhardt, R.J., Probing the interaction of dengue virus envelope protein with heparin: Assessment of glycosaminoglycan- derived inhibitors (2001) J Med Chem, 44, pp. 2178-2187
  • Martínez-Barragán, J.J., del Angel, R.M., Identification of a putative coreceptor on Vero cells that participates in dengue 4 virus infection (2001) J Virol, 75, pp. 7818-7827
  • Ono, L., Wollinger, W., Rocco, I.M., Coimbra, T.L., Gorin, P.A., Sierakowski, M.R., In vitro and in vivo antiviral properties of sulfated galactomannans against yellow fever virus (BeH111 strain) and dengue 1 virus (Hawaii strain) (2003) Antiviral Res, 60, pp. 201-208
  • Paingankar, M.S., Gokhale, M.D., Deobagkar, D.N., Dengue-2-virus-interacting polypeptides involved in mosquito cell infection (2010) Arch Virol, 155, pp. 1453-1461
  • Pujol, C.A., Estevez, J.M., Carlucci, M.J., Ciancia, M., Cerezo, A.S., Damonte, E.B., Novel DL-galactan hybrids from the red seaweed Gymnogongrus torulosus are potent inhibitors of herpes simplex virus and dengue virus (2002) Antivir Chem Chemother, 13, pp. 83-89
  • Qiu, H., Tang, W., Tong, X., Ding, K., Zuo, J., Structure elucidation and sulfated derivatives preparation of two a-D-glucans from Gastrodia elata Bl. and their anti-dengue virus bioactivities (2007) Carbohydr Res, 342, pp. 2230-2236
  • Rostand, K.S., Esko, J.D., Microbial adherence to and invasion through proteoglycans (1997) Infect Immun, 65, pp. 1-8
  • Salas-Benito, J., del Reyes-Valle, J., Salas-Benito, M., Ceballos-Olvera, I., Mosso, C., del Angel, R.M., Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock related protein (2007) Am J Trop Med Hyg, 77, pp. 283-290
  • Sessions, O.M., Barrows, N.J., Souza-Neto, J.A., Robinson, T.J., Hershey, C.L., Rodgers, M.A., Ramirez, J.L., Yang, P.L., Discovery of insect and human dengue virus host factors (2009) Nature, 458, pp. 1047-1050
  • Sinnis, P., Coppi, A., Toida, T., Toyoda, H., Kinoshita-Toyoda, A., Xie, J., Kemp, M.M., Linhardt, R.J., Mosquito heparan sulfate and its potential role in malaria infection and transmission (2007) J Biol Chem, 282, pp. 25376-25384
  • Spillmann, D., Heparan sulfate: Anchor for viral intruders? (2001) Biochimie, 83, pp. 811-817
  • Talarico, L.B., Damonte, E.B., Interference in dengue virus adsorption and uncoating by carrageenans (2007) Virology, 363, pp. 473-485
  • Talarico, L.B., Pujol, C.A., Zibetti, R.G., Faría, P.C., Noseda, M.D., Duarte, M.E., Damonte, E.B., The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell (2005) Antiviral Res, 66, pp. 103-110
  • Talarico, L.B., Duarte, M.E., Zibetti, R.G., Noseda, M.D., Damonte, E.B., An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection (2007) Planta Med, 73, pp. 1464-1468
  • Thaisomboonsuk, B.K., Clayson, E.T., Pantuwatana, S., Vaughn, D.W., Endy, T.P., Characterization of dengue-2 virus binding to surfaces of mammalian and insect cells (2005) Am J Trop Med Hyg, 72, pp. 375-383

Citas:

---------- APA ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R. & Damonte, E.B. (2011) . Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan. Journal of General Virology, 92(6), 1332-1342.
http://dx.doi.org/10.1099/vir.0.028522-0
---------- CHICAGO ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. "Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan" . Journal of General Virology 92, no. 6 (2011) : 1332-1342.
http://dx.doi.org/10.1099/vir.0.028522-0
---------- MLA ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. "Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan" . Journal of General Virology, vol. 92, no. 6, 2011, pp. 1332-1342.
http://dx.doi.org/10.1099/vir.0.028522-0
---------- VANCOUVER ----------
Talarico, L.B., Noseda, M.D., Ducatti, D.R.B., Duarte, M.E.R., Damonte, E.B. Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan. J. Gen. Virol. 2011;92(6):1332-1342.
http://dx.doi.org/10.1099/vir.0.028522-0