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Abstract:

Several disulfide-based and azoic compounds have shown antiviral and virucidal properties against arenaviruses in virus yield-inhibition and inactivation assays, respectively. The most effective virucidal agent, the aromatic disulfide NSC20625, was able to inactivate two strains of the prototype arenavirus species Lymphocytic choriomeningitis virus (LCMV). Inactivated viral particles retained the biological functions of the virion envelope glycoproteins in virus binding and uptake, but were unable to perform viral RNA replication. Furthermore, in inactivated virions, the electrophoretic profile of the Z protein was altered when analysed under non-reducing conditions, whereas the patterns of the proteins NP and GP1 remained unaffected. Treatment of a recombinant LCMV Z protein with the virucidal agents induced unfolding and oligomerization of Z to high-molecular-mass aggregates, probably due to metal-ion ejection and the formation of intermolecular disulfide bonds through the cysteine residues of the Z RING finger. NSC20625 also exhibited antiviral properties in LCMV-infected cells without affecting other cellular RING-motif proteins, such as the promyelocytic leukaemia protein PML. Altogether, the investigations described here illustrate the potential of the Z protein as a promising target for therapy and the prospects of the Z-reactive compounds to prevent arenavirus dissemination. © 2006 SGM.

Registro:

Documento: Artículo
Título:Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger-reactive compounds
Autor:García, C.C.; Djavani, M.; Topisirovic, I.; Borden, K.L.B.; Salvato, M.S.; Damonte, E.B.
Filiación:Laboratory of Virology, Department of Biological Chemistry, University of Buenos Aires, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Institute of Human Virology, University of Maryland, Biotechnology Center, Baltimore, MD 21201, United States
Institute de Recherche en Immunologie et en Cancerologie, Université de Montréal, Montreal, Que. H3T 1J4, Canada
Palabras clave:1 (2 guanidine)phenyldisulfide; 1,2 dithiane 4,5 diol 1,1 dioxide; 3 nitrophenyldisulfide; aldrithiol 2; antiinfective agent; antivirus agent; aromatic compound; azoformamide; bis[2 [n' (2,4 dichlorobenzal)]carboxamide 1 benzene]disulfide; cysteine; disulfide; fatty acid binding protein; metal ion; nsc 203; nsc 20625; nsc 4493; nsc 624151; nsc 624152; promyelocytic leukemia protein; recombinant protein; ribavirin; unclassified drug; virus envelope protein; virus glycoprotein; virus protein; virus RNA; zinc finger protein; antiviral activity; Arenavirus; article; controlled study; disulfide bond; drug efficacy; drug targeting; electrophoresis; human; human cell; Lymphocytic choriomeningitis virus; molecular weight; nonhuman; oligomerization; priority journal; protein aggregation; protein function; protein motif; RNA replication; virion; virus inactivation; virus infection; virus inhibition; virus particle; virus strain; Animals; Azo Compounds; Carrier Proteins; Cell Line; Disulfides; Humans; Lymphocytic choriomeningitis virus; Oligodeoxyribonucleotides; RNA, Viral; Virus Replication; Zinc Fingers; Arenavirus; Lymphocytic choriomeningitis virus
Año:2006
Volumen:87
Número:5
Página de inicio:1217
Página de fin:1228
DOI: http://dx.doi.org/10.1099/vir.0.81667-0
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
ISSN:00221317
CODEN:JGVIA
CAS:azoformamide, 123-77-3; cysteine, 4371-52-2, 52-89-1, 52-90-4; disulfide, 16734-12-6; ribavirin, 36791-04-5; Azo Compounds; Carrier Proteins; Disulfides; Oligodeoxyribonucleotides; p11 Z protein, Lymphocytic choriomeningitis virus; RNA, Viral
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v87_n5_p1217_Garcia

Referencias:

  • Albiol Matanic, V.C., Castilla, V., Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus (2004) Int J Antimicrob Agents, 23, pp. 382-389
  • Andrei, G., De Clercq, E., Inhibitory effect of selected antiviral compounds on arenavirus replication in vitro (1990) Antiviral Res, 14, pp. 287-299
  • Auperin, D.D., Romanowski, V., Galinski, M., Bishop, D.H.L., Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA (1984) J Virol, 52, pp. 897-904
  • Bartolotta, S., García, C.C., Candurra, N.A., Damonte, E.B., Effect of fatty acids on arenavirus replication: Inhibition of virus production by lauric acid (2001) Arch Virol, 146, pp. 777-790
  • Borden, K.L.B., Boddy, M.N., Lally, J., O'Reilly, N.J., Martin, S., Howe, K., Solomon, E., Freemont, P.S., The solution structure of the RING finger domain from the acute promyelocytic leukaemia proto-oncoprotein PML (1995) EMBO J, 14, pp. 1532-1541
  • Borden, K.L.B., Campbell Dwyer, E.J., Carlile, G.W., Djavani, M., Salvato, M.S., Two RING finger proteins, the oncoprotein PML and the arenavirus Z protein, colocalize with the nuclear fraction of the ribosomal P proteins (1998) J Virol, 72, pp. 3819-3826
  • Borden, K.L.B., Campbell Dwyer, E.J., Salvato, M.S., An arenavirus RING (zinc-binding) protein binds the oncoprotein promyelocyte leukemia protein (PML) and relocates PML nuclear bodies to the cytoplasm (1998) J Virol, 72, pp. 758-766
  • Brzovic, P.S., Meza, J., King, M.C., Klevit, R.E., The cancer-predisposing mutation C61G disrupts homodimer formation in the NH2-terminal BRCA1 RING finger domain (1998) J Biol Chem, 273, pp. 7795-7799
  • Burns III, N.J., Barnett, B.B., Huffman, J.H., Dawson, M.I., Sidwell, R.W., De Clercq, E., Kende, M., A newly developed immunofluorescent assay for determining the Pichinde virus-inhibitory effects of selected nucleoside analogues (1988) Antiviral Res, 10, pp. 89-98
  • Campbell Dwyer, E.J., Lai, H., MacDonald, R.C., Salvato, M.S., Borden, K.L.B., The lymphocytic choriomeningitis virus RING protein Z associates with eukaryotic initiation factor 4E and selectively represses translation in a RING-dependent manner (2000) J Virol, 74, pp. 3293-3300
  • Candurra, N.A., Maskin, L., Damonte, E.B., Inhibition of arenavirus multiplication in vitro by phenotiazines (1996) Antiviral Res, 31, pp. 149-158
  • Capili, A.D., Schultz, D.C., Rauscher III, F.J., Borden, K.L.B., Solution structure of the PHD domain from the KAP-1 corepressor: Structural determinants for PHD, RING and LIM zinc-binding domains (2001) EMBO J, 20, pp. 165-177
  • Castilla, V., Barquero, A.A., Mersich, S.E., Coto, C.E., In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves (1998) Int J Antimicrob Agents, 10, pp. 67-75
  • Charrel, R.N., Lemasson, J.-J., Garbutt, M., Khelifa, R., De Micco, P., Feldmann, H., de Lamballerie, X., New insights into the evolutionary relationships between arenaviruses provided by comparative analysis of small and large segment sequences (2003) Virology, 317, pp. 191-196
  • Chen, X., Chu, M., Giedroc, D.P., Spectroscopic characterization of Co(II)-, Ni(II)-, and Cd(II) -substituted wild-type and non-native retroviral-type zinc finger peptides (2000) J Biol Inorg Chem, 5, pp. 93-101
  • Clegg, J.C.S., Molecular phylogeny of the arenaviruses (2002) Curr Top Microbiol Immunol, 262, pp. 1-24
  • Cordo, S.M., Candurra, N.A., Damonte, E.B., Myristic acid analogs are inhibitors of Junin virus replication (1999) Microbes Infect, 1, pp. 609-614
  • Cornu, T.I., de la Torre, J.C., RING finger Z protein of lymphocytic choriomeningitis virus (LCMV) inhibits transcription and RNA replication of an LCMV S-segment minigenome (2001) J Virol, 75, pp. 9415-9426
  • Cornu, T.I., de la Torre, J.C., Characterization of the arenavirus RING finger Z protein regions required for Z-mediated inhibition of viral RNA synthesis (2002) J Virol, 76, pp. 6678-6688
  • Damonte, E.B., Coto, C.E., Treatment of arenavirus infections: From basic studies to the challenge of antiviral therapy (2002) Adv Virus Res, 58, pp. 125-155
  • Damonte, E.B., Mersich, S.E., Candurra, N.A., Intracellular processing and transport of Junin virus glycoproteins influences virion infectivity (1994) Virus Res, 34, pp. 317-326
  • Djavani, M., Topisirovic, I., Zapata, J.C., The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus (2005) J Virol, 79, pp. 2461-2473. , & 8 other authors
  • Eichler, R., Lenz, O., Strecker, T., Eickmann, M., Klenk, H.-D., Garten, W., Identification of Lassa virus glycoprotein signal peptide as a trans-acting maturation factor (2003) EMBO Rep, 4, pp. 1084-1088
  • Eichler, R., Lenz, O., Strecker, T., Garten, W., Signal peptide of Lassa virus glycoprotein GP-C exhibits an unusual length (2003) FEBS Lett, 538, pp. 203-206
  • Eichler, R., Strecker, T., Kolesnikova, L., ter Meulen, J., Weissenhorn, W., Becker, S., Klenk, H.D., Lenz, O., Characterization of the Lassa virus matrix protein Z: Electron microscopic study of virus-like particles and interaction with the nucleoprotein (NP) (2004) Virus Res, 100, pp. 249-255
  • Enría, D.A., Maiztegui, J.I., Antiviral treatment of Argentine hemorrhagic fever (1994) Antiviral Res, 23, pp. 23-31
  • Fasman, G.D., (1996) Circular Dichroism and the Conformational Analysis of Biomolecules, , (editor) New York: Plenum
  • Franze-Fernández, M.T., Iapalucci, S., López, N., Rossi, C., Subgenomic RNAs of Tacaribe virus (1993) The Arenaviridae, pp. 113-132. , Edited by M. S. Salvato. New York: Plenum
  • Freed, E.O., Viral late domains (2002) J Virol, 76, pp. 4679-4687
  • García, C.C., Candurra, N.A., Damonte, E.B., Antiviral and virucidal activities against arenaviruses of zinc-finger active compounds (2000) Antivir Chem Chemother, 11, pp. 231-238
  • García, C.C., Candurra, N.A., Damonte, E.B., Mode of inactivation of arenaviruses by disulfide-based compounds (2002) Antiviral Res, 55, pp. 437-446
  • García, C.C., Rosso, M.L., Bertoni, M.D., Maier, M.S., Damonte, E.B., Evaluation of the antiviral activity against Junin virus of macrocyclic trichothecenes produced by the hypocrealean epibiont of Baccharis coridifolia (2002) Planta Med, 68, pp. 209-212
  • García, C.C., Brousse, B.N., Carlucci, M.J., Moglioni, A.G., Martins Alho, M., Moltrasio, G.Y., D'Accorso, N.B., Damonte, E.B., Inhibitory effect of thiosemicarbazone derivatives on Junin virus replication in vitro (2003) Antivir Chem Chemother, 14, pp. 99-105
  • García, C.C., Candurra, N.A., Damonte, E.B., Differential inhibitory action of two azoic compounds against arenaviruses (2003) Int J Antimicrob Agents, 21, pp. 319-324
  • Garcin, D., Rochat, S., Kolakofsky, D., The Tacaribe arenavirus small zinc finger protein is required for both mRNA synthesis and genome replication (1993) J Virol, 67, pp. 807-812
  • Grande-Pérez, A., Lázaro, E., Lowenstein, P., Domingo, E., Manrubia, S.C., Suppression of viral infectivity through lethal defection (2005) Proc Natl Acad Sci U S A, 102, pp. 4448-4452
  • Guillerm, G., Guillerm, D., Vandenplas-Vitkowski, C., Glapski, C., De Clercq, E., Inactivation of S-adenosyl-L-homocysteine hydrolase with novel 5′-thioadenosine derivatives. Antiviral effects (2003) Bioorg Med Chem Lett, 13, pp. 1649-1652
  • Hass, M., Gölnitz, U., Müller, S., Becker-Ziaja, B., Günther, S., Replicon system for Lassa virus (2004) J Virol, 78, pp. 13793-13803
  • Howard, C.R., Antigenic diversity among the arenaviruses (1993) The Arenaviridae, pp. 37-49. , Edited by M. S. Salvato. New York: Plenum
  • Huang, M., Maynard, A., Turpin, J.A., Graham, L., Janini, G.M., Covell, D.G., Rice, W.G., Anti-HIV agents that selectively target retroviral nucleocapsid protein zinc fingers without affecting cellular zinc finger proteins (1998) J Med Chem, 41, pp. 1371-1381
  • Huggins, J.W., Robins, R.K., Canonico, P.G., Synergistic antiviral effects of ribavirin and the C-nucleoside analogs tiazofurin and selenazofurin against togaviruses, bunyaviruses, and arenaviruses (1984) Antimicrob Agents Chemother, 26, pp. 476-480
  • Kentsis, A., Gordon, R.E., Borden, K.L.B., Self-assembly properties of a model RING domain (2002) Proc Natl Acad Sci U S A, 99, pp. 667-672
  • López, N., Jácamo, R., Franze-Fernández, M.T., Transcription and RNA replication of Tacaribe virus genome and antigenome analogs require N and L proteins: Z protein is an inhibitor of these processes (2001) J Virol, 75, pp. 12241-12251
  • McCormick, J.B., Fisher-Hoch, S.P., Lassa fever (2002) Curr Top Microbiol Immunol, 262, pp. 75-109
  • McCormick, J.B., King, I.J., Webb, P.A., Scribner, C.L., Craven, R.B., Johnson, K.M., Elliot, L.H., Belmont-Williams, R., Lassa fever. Effective therapy with ribavirin (1986) N Engl J Med, 314, pp. 20-26
  • McKee Jr., K.T., Huggins, J.W., Trahan, C.J., Mahlandi, B.G., Ribavirin prophylaxis and therapy for experimental Argentine hemorrhagic fever (1988) Antimicrob Agents Chemother, 32, pp. 1304-1309
  • Miller Jenkins, L.M., Byrd, J.C., Hare, T., Studies on the mechanism of inactivation of the HIV-1 nucleocapsid protein NCp7 with 2-mercaptobenzamide thioesters (2005) J Med Chem, 48, pp. 2847-2858. , & 7 other authors
  • Neuman, B.W., Adair, B.D., Burns, J.W., Milligan, R.A., Buchmeier, M.J., Yeager, M., Complementarity in the supramolecular design of arenaviruses and retroviruses revealed by electron cryomicroscopy and image analysis (2005) J Virol, 79, pp. 3822-3830
  • Perez, M., Craven, R.C., de la Torre, J.C., The small RING finger protein Z drives arenavirus budding: Implications for antiviral strategies (2003) Proc Natl Acad Sci U S A, 100, pp. 12978-12983
  • Perez, M., Greenwald, D.L., de la Torre, J.C., Myristoylation of the RING finger Z protein is essential for arenavirus budding (2004) J Virol, 78, pp. 11443-11448
  • Peters, C.J., Human infection with arenaviruses in the Americas (2002) Curr Top Microbiol Immunol, 262, pp. 65-74
  • Rein, A., Ott, D.E., Mirro, J., Arthur, L.O., Rice, W., Henderson, L.E., Inactivation of murine leukemia virus by compounds that react with the zinc finger in the viral nucleocapsid protein (1996) J Virol, 70, pp. 4966-4972
  • Rice, W.G., Turpin, J.A., Schaeffer, C.A., Evaluation of selected chemotypes in coupled cellular and molecular target-based screens identifies novel HIV-1 zinc finger inhibitors (1996) J Med Chem, 39, pp. 3606-3616. , & 7 other authors
  • Roehm, P.C., Berg, J.M., Sequential metal binding by the RING finger domain of BRCA1 (1997) Biochemistry, 36, pp. 10240-10245
  • Rotz, L.D., Khan, A.S., Lillibridge, S.R., Ostroff, S.M., Hughes, J.M., Public health assessment of potential biological terrorism agents (2002) Emerg Infect Dis, 8, pp. 225-230
  • Salvato, M.S., Molecular biology of the prototype arenavirus, lymphocytic choriomeningitis virus (1993) The Arenaviridae, pp. 133-156. , Edited by M. S. Salvato. New York: Plenum
  • Salvato, M.S., Shimomaye, E.M., The completed sequence of lymphocytic choriomeningitis virus reveals a unique RNA structure and a gene for a zinc finger protein (1989) Virology, 173, pp. 1-10
  • Salvato, M.S., Schweighofer, K.J., Burns, J., Shimomaye, E.M., Biochemical and immunological evidence that the 11 kDa zinc-binding protein of lymphocytic choriomeningitis virus is a structural component of the virus (1992) Virus Res, 22, pp. 185-198
  • Salvato, M.S., Clegg, J.C.S., Buchmeier, M.J., Charrel, R.N., Gonzalez, J.-P., Lukashevich, I.S., Peters, C.J., Romanowski, V., Arenaviridae (2005) Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 725-733. , Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press
  • Sánchez, A.B., Perez, M., Cornu, T., de la Torre, J.C., RNA interference-mediated virus clearance from cells both acutely and chronically infected with the prototypic arenavirus lymphocytic choriomeningitis virus (2005) J Virol, 79, pp. 11071-11081
  • Smee, D.F., Morris, J.L., Barnard, D.L., Van Aerschot, A., Selective inhibition of arthropod-borne and arenaviruses in vitro by 3′-fluoro-3′-deoxyadenosine (1992) Antiviral Res, 18, pp. 151-162
  • Smee, D.F., Gilbert, J., Leonhardt, J.A., Barnett, B.B., Huggins, J.H., Sidwell, R.W., Treatment of lethal Pichinde virus infections in weanling LVG/Lak hamsters with ribavirin, ribamidine, selenazofurin, and ampligen (1993) Antiviral Res, 20, pp. 57-70
  • Strecker, T., Eichler, R., ter Meulen, J., Weissenhorn, W., Klenk, H.D., Garten, W., Lenz, O., Lassa virus Z protein is a matrix protein sufficient for the release of virus-like particles (2003) J Virol, 77, pp. 10700-10705
  • Topisirovic, I., Capili, A.D., Borden, K.L.B., Gamma interferon and cadmium treatments modulate eukaryotic initiation factor 4E-dependent mRNA transport of cyclin D1 in a PML-dependent manner (2002) Mol Cell Biol, 22, pp. 6183-6198
  • Tortorici, M.A., Ghiringhelli, P.D., Lozano, M.E., Albariño, C.G., Romanowski, V., Zinc-binding properties of Junín virus nucleocapsid protein (2001) J Gen Virol, 82, pp. 121-128
  • Tummino, P.J., Harvey, P.J., McQuade, T., Domagala, J., Gogliotti, R., Sanchez, J., Song, Y., Hupe, D., The human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein zinc ejection activity of disulfide benzamides and benzisothiazolones: Correlation with anti-HIV and virucidal activities (1997) Antimicrob Agents Chemother, 41, pp. 394-400
  • Turpin, J.A., Song, Y., Inman, J.K., Huang, M., Wallqvist, A., Maynard, A., Covell, D.G., Appella, E., Synthesis and biological properties of novel pyridinioalkanoyl thiolesters (PATE) as anti-HIV-1 agents that target the viral nucleocapsid protein zinc fingers (1999) J Med Chem, 42, pp. 67-86
  • Uckun, F.M., Petkevich, A.S., Vassilev, A.O., Tibbles, H.E., Titov, L., Stampidine prevents mortality in an experimental mouse model of viral hemorrhagic fever caused by Lassa virus (2004) BMC Infect Dis, 4, p. 1
  • Wachsman, M.B., López, E.M.F., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral effect of brassinosteroids against herpes virus and arenaviruses (2000) Antivir Chem Chemother, 11, pp. 71-77
  • Witvrouw, M., Desmyter, J., De Clercq, E., Antiviral portrait series. 4. Polysulfates as inhibitors of HIV and other enveloped viruses (1994) Antivir Chem Chemother, 5, pp. 345-359
  • Wulff, H., Lange, J.V., Webb, P.A., Interrelationships among arenaviruses measured by indirect immunofluorescence (1978) Intervirology, 9, pp. 344-350

Citas:

---------- APA ----------
García, C.C., Djavani, M., Topisirovic, I., Borden, K.L.B., Salvato, M.S. & Damonte, E.B. (2006) . Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger-reactive compounds. Journal of General Virology, 87(5), 1217-1228.
http://dx.doi.org/10.1099/vir.0.81667-0
---------- CHICAGO ----------
García, C.C., Djavani, M., Topisirovic, I., Borden, K.L.B., Salvato, M.S., Damonte, E.B. "Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger-reactive compounds" . Journal of General Virology 87, no. 5 (2006) : 1217-1228.
http://dx.doi.org/10.1099/vir.0.81667-0
---------- MLA ----------
García, C.C., Djavani, M., Topisirovic, I., Borden, K.L.B., Salvato, M.S., Damonte, E.B. "Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger-reactive compounds" . Journal of General Virology, vol. 87, no. 5, 2006, pp. 1217-1228.
http://dx.doi.org/10.1099/vir.0.81667-0
---------- VANCOUVER ----------
García, C.C., Djavani, M., Topisirovic, I., Borden, K.L.B., Salvato, M.S., Damonte, E.B. Arenavirus Z protein as an antiviral target: Virus inactivation and protein oligomerization by zinc finger-reactive compounds. J. Gen. Virol. 2006;87(5):1217-1228.
http://dx.doi.org/10.1099/vir.0.81667-0