Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Junín virus (JUNV), the causative agent of Argentine haemorrhagic fever, is a human pathogen that naturally enters the body through the epithelial cells of the respiratory and digestive tracts. The interaction of JUNV with two types of polarized epithelial cultures, Vero C1008 and A549, was investigated. Radioactive virus-binding assays showed that JUNV infects polarized lines preferentially through the apical surface. High-level expression of viral nucleoprotein was detected in polarized cell lines infected through the apical domain. Virus production from apical media was about 100-fold higher than that found into the basolateral medium. Confocal-immunofluorescence analysis revealed high-level expression of glycoprotein at the apical-membrane surface. Disruption of the microtubule network by colchicine impaired JUNV vectorial release. This is the first study to analyse the interaction between a member of the virus family Arenaviridae and polarized epithelial cells, showing preferential entry and release from the apical plasma membrane. © 2005 SGM.


Documento: Artículo
Título:Polarized entry and release of Junín virus, a New World arenavirus
Autor:Cordo, S.M.; Cesio Acuña, M.; Candurra, N.A.
Filiación:Laboratorio de Verología, Departamento de Química Biológica, Universidad de Buenos Aires, CP 1428, Buenos Aires, Argentina
Palabras clave:colchicine; virus glycoprotein; virus nucleoprotein; animal cell; Arenavirus; article; binding assay; cell culture; cell membrane transport; cell surface; confocal microscopy; controlled study; culture medium; epithelium cell; human; human cell; immunofluorescence microscopy; intestine epithelium; Junin virus; microtubule; nonhuman; polarization; priority journal; protein expression; respiratory epithelium; stomach epithelium; Vero cell; virogenesis; virus cell interaction; virus hemorrhagic fever; virus transmission; Animals; Cell Membrane; Cell Polarity; Cells, Cultured; Cercopithecus aethiops; Epithelial Cells; Gene Expression; Humans; Junin virus; Microscopy, Confocal; Nucleocapsid Proteins; Vero Cells; Arenaviridae; Arenavirus; Argentina (fish); Junin virus
Página de inicio:1475
Página de fin:1479
Título revista:Journal of General Virology
Título revista abreviado:J. Gen. Virol.
CAS:colchicine, 64-86-8; Nucleocapsid Proteins


  • Blau, D.M., Compans, R.W., Entry and release of measles virus are polarized in epithelial cells (1995) Virology, 210, pp. 91-99
  • Bose, S., Malur, A., Banerjee, A.K., Polarity of human parainfluenza virus type 3 infection in polarized human lung epithelial A549 cells: Role of microfilament and microtubule (2001) J. Virol., 75, pp. 1984-1989
  • Candurra, N.A., Lago, M.J., Maskin, L., Damonte, E.B., Involvement of the cytoskeleton in Junin virus multiplication (1999) J. Gen. Virol., 80, pp. 147-156
  • Cao, W., Henry, M.D., Borrow, P., Identification of α-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus (1998) Science, 282, pp. 2079-2081. , 7 other authors
  • Chen, S.Y., Matsuoka, Y., Compans, R.W., Assembly and polarized relese of Punta Toro virus and effects of brefeldin A (1991) J. Virol., 65, pp. 1427-1439
  • Chu, J.J.H., Ng, M.L., Infection of polarized epithelial cells with flavivirus West Nile: Polarized entry and egress of virus occur though the apical surface (2002) J. Gen. Virol., 83, pp. 2427-2435
  • Clayson, E.T., Brando, L.V.J., Compans, R.W., Release of simian virus 40 virions from epithelial cells is polarized and occurs without cell lysis (1989) J. Virol., 63, pp. 2278-2288
  • Clegg, J.C., Molecular phylogeny of the arenaviruses (2002) Curr. Top Microbiol. Immunol., 262, pp. 1-24
  • Contigiani, M.S., Sabattini, M.S., Virulencia diferencial de cepas de virus Junín por marcadores Biológicos en ratones y cobayos (1977) Medicina (B Aires), 37, pp. 244-251. , (in Spanish)
  • Cordo, S.M., Candurra, N.A., Intermediate filament integrity is required for Junin virus replication (2003) Virus Res., 97, pp. 47-55
  • Damonte, E.B., Mersich, S.E., Candurra, N.A., Intracellular processing and transport of Junin virus glycoproteins influences virion infectivity (1994) Virus Res., 34, pp. 317-326
  • Fuller, S., von Bonsdorff, C.-H., Simons, K., Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK (1984) Cell, 38, pp. 65-77
  • Gonzalez, P.H., Cossio, P.M., Arana, R., Maiztegui, J.I., Laguens, R.P., Lymphatic tissue in Argentine hemorrhagic fever. Pathologic features (1980) Arch. Pathol. Lab. Med., 104, pp. 250-254
  • Guadiz, G., Sporn, L.A., Goss, R.A., Lawrence, S.O., Marder, V.J., Simpson-Haidaris, P.J., Polarized secretion of fibrinogen by lung epithelial cells (1997) Am. J. Respir. Cell Mol. Biol., 17, pp. 60-69
  • Jacob, R., Heine, M., Alfalah, M., Naim, H.Y., Distinct cytoskeletal tracks direct individual vesicle populations to the apical membrane of epithelial cells (2003) Curr. Biol., 13, pp. 607-612
  • Lafont, F., Burkhardt, J.K., Simons, K., Involvement of microtubule motors in basolateral and apical transport in kidney cells (1994) Nature, 372, pp. 801-803
  • Laguens, M., Chambó, J.G., Laguens, R.P., In vivo replication of pathogenic and attenuated strains of Junin virus in different cell populations of lymphatic tissue (1983) Infect. Immun., 41, pp. 1279-1283
  • Marozin, S., Prank, U., Sodeik, B., Herpes simplex virus type 1 infection of polarized epithelial cells requires microtubules and access to receptors present at cell-cell contact sites (2004) J. Gen. Virol., 85, pp. 775-786
  • Neely, J.D., Amiry-Moghaddam, M., Ottersen, O.P., Froehner, S.C., Agre, P., Adams, M.E., Syntrophin-dependent expression and localization of Aquaporin-4 water channel protein (2001) Proc. Natl. Acad. Sci. U. S. A., 98, pp. 14108-14113
  • Parodi, A., Greenway, D., Rugiero, H.R., Sobre la etiología del brote epidémico de Junín (1958) Dia. Med., 30, pp. 2300-2302. , 7 other authors (in Spanish)
  • Romanowski, V., Genetic organization of Junín virus, the etiological agent of Argentine hemorrhagic fever (1993) The Arenaviridae, pp. 51-83. , Edited by M. S. Salvato. New York: Plenum
  • Rossen, J.W.A., Bekker, C.P.J., Voorhout, W.F., Strous, G.J.A.M., van der Ende, A., Rottier, P.J.M., Entry and release of transmissible gastroenteritis coronavirus are restricted to apical surfaces of polarized epithelial cells (1994) J. Virol., 68, pp. 7966-7973
  • Schneider-Schaulies, J., Cellular receptors for viruses: Links to tropism and pathogenesis (2000) J. Gen. Virol., 81, pp. 1413-1429
  • Scolaro, L.A., Mersich, S.E., Damonte, E.B., Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells (1989) Virus Res., 13, pp. 283-294
  • Spiropoulou, C.F., Kunz, S., Rollin, P.E., Campbell, K.P., Oldstone, M.B.A., New World arenavirus clade C, but not clade A and B viruses, utilizes α-dystroglycan as its major receptor (2002) J. Virol., 76, pp. 5140-5146
  • Srinivas, R.V., Balachandran, N., Alonso-Caplen, F.V., Compans, R.W., Expression of herpes simplex virus glycoproteins in polarized epithelial cells (1986) J. Virol., 58, pp. 689-693
  • Weissenbacher, M.C., Laguens, R.P., Coto, C.E., Argentine hemorrhagic fever (1987) Curr. Top Microbiol. Immunol., 134, pp. 79-116
  • Zegers, M.M.P., Zaal, K.J.M., van IJzendoorn, S.C.D., Klappe, K., Hoekstra, D., Actin filaments and microtubules are involved in different membrane traffic pathways that transport sphingolipids to the apical surface of polarized HepG2 cells (1998) Mol. Biol. Cell, 9, pp. 1939-1949


---------- APA ----------
Cordo, S.M., Cesio Acuña, M. & Candurra, N.A. (2005) . Polarized entry and release of Junín virus, a New World arenavirus. Journal of General Virology, 86(5), 1475-1479.
---------- CHICAGO ----------
Cordo, S.M., Cesio Acuña, M., Candurra, N.A. "Polarized entry and release of Junín virus, a New World arenavirus" . Journal of General Virology 86, no. 5 (2005) : 1475-1479.
---------- MLA ----------
Cordo, S.M., Cesio Acuña, M., Candurra, N.A. "Polarized entry and release of Junín virus, a New World arenavirus" . Journal of General Virology, vol. 86, no. 5, 2005, pp. 1475-1479.
---------- VANCOUVER ----------
Cordo, S.M., Cesio Acuña, M., Candurra, N.A. Polarized entry and release of Junín virus, a New World arenavirus. J. Gen. Virol. 2005;86(5):1475-1479.