Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6 passages, a cyclical pattern was observed between passages 6 and 16, and subsequently no virus infectivity could be detected. Co-cultivation with normal RK-13 or Vero cells enhanced virus yield from virus-producing cultures of Veri-T cells (passage 15), but the addition of susceptible cells had no effect on non-producer Vero-T cultures (passage 19). Only a small proportion (<1%) of the persistently infected cells tested during the first 16 passages produced infectious virus. The virus released during the early stages of persistence was temperature-sensitive if grown at 40°C, more thermolabile at 50°C than parental virus, and unable to initiate a persistent infection in Vero cells. Vero-T cells consistently showed refractoriness to homotypic Tacaribe virus superinfection and a selective graded resistance to other arenavirus replication. The possible use of viral susceptibility of persistently infected cultures as marker of antigenic relationship among Tacaribe complex viruses is considered.
Documento: | Artículo |
Título: | Persistent infection of Vero cells with Tacaribe virus |
Autor: | Damonte, E.B.; D'Aiutola, C.; Coto, C.E. |
Filiación: | Cat. Virol., Dept. Quim. Biol., Fac. Ci. Exact. Nat., Univ., 1428 Buenos Aires, Argentina |
Palabras clave: | animal experiment; cell culture; in vitro study; persistent infection; tacaribe arenavirus; Animalia; Arenavirus; Tacaribe virus |
Año: | 1981 |
Volumen: | 56 |
Número: | 1 |
Página de inicio: | 41 |
Página de fin: | 48 |
DOI: | http://dx.doi.org/10.1099/0022-1317-56-1-41 |
Título revista: | Journal of General Virology |
Título revista abreviado: | J. GEN. VIROL. |
ISSN: | 00221317 |
CODEN: | JGVIA |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221317_v56_n1_p41_Damonte |