Artículo
Abstract:
Acetobacter xylinum strain NRRL B42 and its derivative RCGr1 produce a complex exopolysaccharide, acetan, containing glucose, mannose, glucuronic acid and rhamnose in a 4:1:1:1 molar ratio. The in vitro synthesis of acetan, employing electroporated cells as the enzyme system and the respective 14C-labelled sugar nucleotide precursors, is described. The synthesis of the prenyl-linked heptasaccharide repeat unit, already observed in EDTA-treated cells, was confirmed, as well as the formation of other saccharides not related to acetan biosynthesis, including a high molecular mass glucan. The acetan formed was characterized by gel filtration, specific radioactive labelling with each precursor and permethylation analysis. It was also shown that acetan contains acetyl residues and that using [14C]acetyl CoA as donor, radioactivity was detected both at the polysaccharide and at the prenyl-linked oligosaccharide stage.
Registro:
| Documento: | Artículo |
| Título: | In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations |
| Autor: | Semino, C.E.; Dankert, M.A. |
| Filiación: | Inst Inv Bioquimicas 'Fund Campomar', Facultad Ciencias Exactas Naturales, Consejo Nac Inv Cientificas Tecnicas, Av Patricias Argentinas 435, Buenos Aires 1405, Argentina |
| Palabras clave: | acetic acid derivative; acyl coenzyme A; carbon 14; edetic acid; enzyme; glucan; glucose; glucuronic acid; mannose; oligosaccharide; polysaccharide; prenylamine; rhamnose; sugar nucleotide; Acetobacter; article; carbohydrate synthesis; electroporation; gel filtration; isotope labeling; methylation; molecular weight; nonhuman; precursor; priority journal |
| Año: | 1993 |
| Volumen: | 139 |
| Número: | 11 |
| Página de inicio: | 2745 |
| Página de fin: | 2756 |
| DOI: | http://dx.doi.org/10.1099/00221287-139-11-2745 |
| Título revista: | Journal of General Microbiology |
| Título revista abreviado: | J. GEN. MICROBIOL. |
| ISSN: | 00221287 |
| CODEN: | JGMIA |
| CAS: | carbon 14, 14762-75-5; edetic acid, 150-43-6, 60-00-4; glucan, 9012-72-0, 9037-91-6; glucose, 50-99-7, 84778-64-3; glucuronic acid, 36116-79-7, 576-37-4, 6556-12-3; mannose, 31103-86-3, 3458-28-4; prenylamine, 390-64-7; rhamnose, 10485-94-6, 3615-41-6 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221287_v139_n11_p2745_Semino |
Citas:
---------- APA ----------
Semino, C.E. & Dankert, M.A. (1993) . In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations. Journal of General Microbiology, 139(11), 2745-2756.http://dx.doi.org/10.1099/00221287-139-11-2745
---------- CHICAGO ----------
Semino, C.E., Dankert, M.A. "In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations" . Journal of General Microbiology 139, no. 11 (1993) : 2745-2756.http://dx.doi.org/10.1099/00221287-139-11-2745
---------- MLA ----------
Semino, C.E., Dankert, M.A. "In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations" . Journal of General Microbiology, vol. 139, no. 11, 1993, pp. 2745-2756.http://dx.doi.org/10.1099/00221287-139-11-2745
---------- VANCOUVER ----------
Semino, C.E., Dankert, M.A. In vitro biosynthesis of acetan using electroporated Acetobacter xylinum cells as enzyme preparations. J. GEN. MICROBIOL. 1993;139(11):2745-2756.http://dx.doi.org/10.1099/00221287-139-11-2745
