Abstract:
Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.
Registro:
Documento: |
Artículo
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Título: | Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. |
Autor: | Conforte, V.P.; Echeverria, M.; Sánchez, C.; Ugalde, R.A.; Menéndez, A.B.; Lepek, V.C. |
Filiación: | Instituto de Investigaciones Biotecnológicas, INTECH, Universidad Nacional de General San Martín, CONICET, Buenos Aires, Argentina Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
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Palabras clave: | ACC deaminase; Competitiveness; Ethylene; Lotus; Mesorhizobium loti; 1 aminocyclopropanecarboxylic acid; 1 aminocyclopropanecarboxylic acid deaminase; deaminase; ethylene; unclassified drug; 1-aminocyclopropane-1-carboxylate deaminase; lyase; article; bacterial cell; bacterium competence; controlled study; enzyme activity; enzyme degradation; forage; genetic engineering; legume; Lotus; Mesorhizobium loti; nodulation; nonhuman; plant chromosome; promoter region; protein expression; Rhizobiaceae; strain difference; structural gene; symbiosis; wild type; Alphaproteobacteria; enzymology; genetics; microbiology; plant root; symbiosis; Lotus corniculatus var. japonicus; Lotus tenuis; Mesorhizobium loti; Alphaproteobacteria; Carbon-Carbon Lyases; Lotus; Plant Roots; Symbiosis |
Año: | 2010
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Volumen: | 56
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Número: | 4
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Página de inicio: | 331
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Página de fin: | 338
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DOI: |
http://dx.doi.org/10.2323/jgam.56.331 |
Título revista: | Journal of General and Applied Microbiology
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Título revista abreviado: | J. Gen. Appl. Microbiol.
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ISSN: | 00221260
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CODEN: | JGAMA
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CAS: | 1 aminocyclopropanecarboxylic acid, 22059-21-8; deaminase, 9067-84-9; ethylene, 74-85-1; lyase, 9055-04-3; 1-aminocyclopropane-1-carboxylate deaminase; Carbon-Carbon Lyases
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00221260_v56_n4_p331_Conforte.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00221260_v56_n4_p331_Conforte |
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Citas:
---------- APA ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B. & Lepek, V.C.
(2010)
. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. Journal of General and Applied Microbiology, 56(4), 331-338.
http://dx.doi.org/10.2323/jgam.56.331---------- CHICAGO ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C.
"Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp."
Journal of General and Applied Microbiology 56, no. 4
(2010) : 331-338.
http://dx.doi.org/10.2323/jgam.56.331---------- MLA ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C.
"Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp."
Journal of General and Applied Microbiology, vol. 56, no. 4, 2010, pp. 331-338.
http://dx.doi.org/10.2323/jgam.56.331---------- VANCOUVER ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. J. Gen. Appl. Microbiol. 2010;56(4):331-338.
http://dx.doi.org/10.2323/jgam.56.331