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Ethylene inhibits the establishment of symbiosis between rhizobia and legumes. Several rhizobia species express the enzyme ACC deaminase, which degrades the ethylene precursor 1-cyclopropane-1-carboxilate (ACC), leading to reductions in the amount of ethylene evolved by the plant. M. loti has a gene encoding ACC deaminase, but this gene is under the activity of the NifA-RpoN- dependent promoter; thus, it is only expressed inside the nodule. The M. loti structural gene ACC deaminase (acdS) was integrated into the M. loti chromosome under a constitutive promoter activity. The resulting strain induced the formation of a higher number of nodules and was more competitive than the wild-type strain on Lotus japonicus and L. tenuis. These results suggest that the introduction of the ACC deaminase activity within M. loti in a constitutive way could be a novel strategy to increase nodulation competitiveness of the bacteria, which could be useful for the forage inoculants industry.


Documento: Artículo
Título:Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp.
Autor:Conforte, V.P.; Echeverria, M.; Sánchez, C.; Ugalde, R.A.; Menéndez, A.B.; Lepek, V.C.
Filiación:Instituto de Investigaciones Biotecnológicas, INTECH, Universidad Nacional de General San Martín, CONICET, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:ACC deaminase; Competitiveness; Ethylene; Lotus; Mesorhizobium loti; 1 aminocyclopropanecarboxylic acid; 1 aminocyclopropanecarboxylic acid deaminase; deaminase; ethylene; unclassified drug; 1-aminocyclopropane-1-carboxylate deaminase; lyase; article; bacterial cell; bacterium competence; controlled study; enzyme activity; enzyme degradation; forage; genetic engineering; legume; Lotus; Mesorhizobium loti; nodulation; nonhuman; plant chromosome; promoter region; protein expression; Rhizobiaceae; strain difference; structural gene; symbiosis; wild type; Alphaproteobacteria; enzymology; genetics; microbiology; plant root; symbiosis; Lotus corniculatus var. japonicus; Lotus tenuis; Mesorhizobium loti; Alphaproteobacteria; Carbon-Carbon Lyases; Lotus; Plant Roots; Symbiosis
Página de inicio:331
Página de fin:338
Título revista:Journal of General and Applied Microbiology
Título revista abreviado:J. Gen. Appl. Microbiol.
CAS:1 aminocyclopropanecarboxylic acid, 22059-21-8; deaminase, 9067-84-9; ethylene, 74-85-1; lyase, 9055-04-3; 1-aminocyclopropane-1-carboxylate deaminase; Carbon-Carbon Lyases


  • Belimov, A.A., Dodd, I.C., Hortzeas, N., Theobald, J.C., Safronova, V.I., Davies, W.J., Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signaling (2009) New Phytol, 181, pp. 413-423
  • Broughton, W.J., Dilworth, M.J., Control of leghemoglobin synthesis in snake beans (1971) Biochem. J, 125, p. 1075
  • Douglas, C.J., Staneloni, R.J., Rubin, R.A., Nester, E.W., Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region (1985) J. Bacteriol, 161, pp. 850-860
  • D'antuono, A.L., Casabuono, A., Couto, A., Ugalde, R.A., Lepek, V.C., Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis (2005) Mol. Plant-Microbe Interact, 18, pp. 446-457
  • Echeverria, M., Scambato, A.A., Sannazzaro, A., Maiale, S., Ruiz, O., Menéndez, A.B., Phenotypic plasticity with respect to salt stress response by Lotus glaber: The role of its AM fungal and rhizobial symbionts (2008) Mycorrhiza, 18, pp. 317-329
  • Evans, H.M., Hoagland, D.R., The synthesis of vitamin E by plants grown in cultures solution (1927) Am. J. Physiol, 80, pp. 702-704
  • Gage, D.J., Infection and invasion of roots by symbiotic, nitrogen-fixing rhizobia during nodulation of temperate legumes (2004) Microbiol. Mol. Biol. Rev, 68, pp. 280-300
  • Glick, B.R., Penrose, D.M., Li, J., A model for the lowering of plant ethylene concentrations by plant growthpromoting bacteria (1998) J. Theor. Biol, 190, pp. 63-68
  • Kaneko, T., Nakamura, Y., Sato, S., Asamizu, E., Kato, T., Sasamoto, S., Watanabe, A., Tabata, S., Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti (2000) DNA Res, 7, pp. 331-338
  • Kaneko, T., Nakamura, Y., Sato, S., Minamisaura, K., Uchiumi, T., Sasamoto, S., Watanabe, A., Tabata, S., Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110 (2002) DNA Res, 9, pp. 189-197
  • Kovach, M.E., Elzer, P.H., Hill, D.S., Robertson, G.T., Farris, M.A., Roop, R.M., Peterson, K.M., Four new derivatives of the broad-host-range cloning vector pBBR1MCs, carrying different antibiotic-resistance cassettes (1995) Gene, 166, pp. 175-176
  • Lepek, V., Navarro de Navarro, Y., Ugalde, R.A., Synthesis of β(1-2)glucan in Rhizobium loti. Expression of Agrobacterium tumefaciens chvB virulence region (1990) Arch. Microbiol, 155, pp. 35-41
  • Ma, W., Charles, T.C., Glick, B.R., Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in Sinorhizobium meliloti increases its ability to nodulate alfalfa (2004) Appl. Environ. Microbiol, 70, pp. 5891-5897
  • Ma, W., Guinel, F.C., Glick, B.R., Rhizobium leguminosarum biovar viciae 1-aminocyclopropane-1-carboxylate deaminase promotes nodulation of pea plants (2003) Appl. Environ. Microbiol, 69, pp. 4396-4402
  • Maier, R.J., Triplett, E.W., Toward more productive, efficient, and competitive nitrogen-fixing symbiotic bacteria (1996) Crit. Rev. Plant Sci, 15, pp. 191-234
  • Mujica, M.M., Rumi, C.P., A technique of vegetative propagation by stem cuttings was fitted to Lotus tenuis (1998) Lotus Newsl, 29, p. 411
  • Nukui, N., Ezura, H., Minamisawa, K., Transgenic Lotus japonicus with an ethylene receptor gene Cm-ERS/ H70A enhances formation of infection threads and nodule primordia (2004) Plant Cell Physiol, 45, pp. 427-435
  • Nukui, N., Ezura, H., Yuhashi, K., Yasuta, T., Minamisawa, K., Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception on nodulation in Lotus japonicus and Macroptilium atropurpureum (2000) Plant Cell Physiol, 41, pp. 893-897
  • Nukui, N., Minamisawa, K., Ayabe, S., Aoki, T., Expression of the 1-aminocyclopropane-1-carboxylic acid deaminase gene requires symbiotic nitrogen-fixing regulator gene nifA2 in Mesorhizobium loti MAFF303099 (2006) Appl. Environ. Microbiol, 72, pp. 4964-4969
  • Okazaki, S., Nukui, N., Sugawara, M., Minamisawa, K., Rhizobial strategies to enhance symbiotic interactions: Rhizobitoxine and 1-aminociclopropano-1-carboxilate deaminase (2004) Microbes Environ, 19, pp. 99-111
  • Oldroyd, G.E., Engstrom, E.M., Long, S.R., Ethylene inhibits the Nod factor signal transduction pathway of Medicago truncatula (2001) Plant Cell, 13, pp. 1835-1849
  • Penmetsa, R.V., Cook, D.R., A legume ethylene-insensitive mutant hyperinfected by its rhizobial symbiont (1997) Science, 275, pp. 527-530
  • Penrose, D.M., Glick, B.R., Levels of ACC and related compounds in exudate and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria (2001) Can. J. Microbiol, 47, pp. 368-372
  • Penrose, D.M., Glick, B.R., Methods for isolating and characterizing ACC deaminase-containing plant growthpromoting rhizobacteria (2003) Physiol. Plant, 118, pp. 10-15
  • Peters, N.K., Crist-Estes, D.K., Nodule formation is stimulated by the ethylene inhibitor aminoethoxyvinylglycine (1989) Plant Physiol, 91, pp. 690-693
  • Sánchez, C., Iannino, F., Deakin, W.J., Ugalde, R.A., Lepek, V.C., Characterization of the Mesorhizobium loti MAFF303099 type-three protein secretion system (2009) Mol. Plant Microbe-Interact, 22, pp. 519-528
  • Sato, S., Nakamura, Y., Kaneko, T., Kato, T., Nakao, M., Sasamoto, S., Watanabe, A., Tabata, S., Genome structure of the legume Lotus Japonicus (2008) DNA Res, 15, pp. 227-239
  • Schmidt, J.S., Harper, J.E., Hoffman, T.K., Bent, A.F., Regulation of soybean nodulation independent of ethylene signaling (1999) Plant Physiol, 119, pp. 951-959
  • Szczyglowski, K., Stougaard, J., Lotus genome: Pod of gold for legume research (2008) Trends Plant Sci, 13, pp. 515-517
  • Teckle, N.L., Real, D., Colmer, T.D., Growth and ion relations in response to combined salinity and waterlogging in the perennial forage legumes Lotus corniculatus and Lotus glaber (2006) Plant Soil, 289, pp. 369-383
  • Tittabutr, P., Awaya, J.D., Li, Q.X., Borthakur, D., The cloned 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene from Sinorhizobium sp. strain BL3 in Rhizobium sp. strain TAL1145 promotes nodulation and growth of Leucaena leucocephala (2008) Syst. Appl. Microbiol, 31, pp. 141-150
  • Triplett, E.W., Sadowsky, M.J., Genetics of competition for nodulation of legumes (1992) Annu. Rev. Microbiol, 46, pp. 399-428
  • Uchiumi, T., Ohwada, T., Itakura, M., Mitsui, H., Nukui, N., Dawadi, P., Kaneko, T., Minamisawa, K., Expression islands clustered on the symbiosis island of the Mesorhizobium loti genome (2004) J. Bacteriol, 186, pp. 2439-2448
  • Ugalde, J.E., Czibener, C., Feldman, M.F., Ugalde, R.A., Identification and characterization of the Brucella abortus phosphoglucomutase gene: Role of lipopolysaccharide in virulence and intracellular multiplication (2000) Infect. Immun, 68, pp. 5716-5723
  • Vlassak, K.M., Vanderleyden, J., Factors influencing nodule occupancy by inoculant rhizobia (1997) Crit. Rev. Plant Sci, 16, p. 163
  • Wolfgang, R.S., Joseph, C.M., Phillips, D.A., Biotin and other water-soluble vitamins are key growth factors for alfalfa root colonization by Rhizobium meliloti 1021 (1996) Mol. Plant-Microbe Interact, 9, pp. 330-338
  • Yuhashi, K., Ichikawa, N., Ezura, H., Asao, S., Minakawa, Y., Nukui, N., Yasuta, T., Minamisawa, K., Rhizobitoxine production by Bradyrhizobium elkanii enhances nodulation and competitiveness on Macroptilium atropurpureum (2000) Appl. Environ. Microbiol, 66, pp. 2658-2663


---------- APA ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B. & Lepek, V.C. (2010) . Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. Journal of General and Applied Microbiology, 56(4), 331-338.
---------- CHICAGO ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C. "Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp." Journal of General and Applied Microbiology 56, no. 4 (2010) : 331-338.
---------- MLA ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C. "Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp." Journal of General and Applied Microbiology, vol. 56, no. 4, 2010, pp. 331-338.
---------- VANCOUVER ----------
Conforte, V.P., Echeverria, M., Sánchez, C., Ugalde, R.A., Menéndez, A.B., Lepek, V.C. Engineered ACC deaminase-expressing free-living cells of Mesorhizobium loti show increased nodulation efficiency and competitiveness on Lotus spp. J. Gen. Appl. Microbiol. 2010;56(4):331-338.