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Abstract:

The actions of several neuropeptides as hypothalamic mediators in the regulation of Bufo arenarum metamorphosis were investigated. Prometamorphic larvae were injected with 1.5 μg thyrotropin-releasing hormone (TRH), 2 μg ovine corticotropin-releasing factor (oCRF), 2 μg mammalian gonadotropin-releasing hormone (mGnRH), 2 μg human growth hormone-releasing hormone (hGHRH), or Holtfreter solution (control group). Larvae received two injections with the same dose: one at the beginning of the experiment and the other 7 days later. Several morphologic parameters (total length, tail length, wet weight, hind limb length, and metamorphic stages) were measured as indicators of growth and metamorphic development. These measurements were taken in 20 larvae per treatment or control group at the beginning of the experiment, at day 7 and at day 14 when the experiment ended. We observed that only the administration of exogenous CRF stimulated resorption of the tail and accelerated the rate of metamorphosis. In the pituitary of CRF-treated larvae we observed that thyrotropin (TSH) and adrenocorticotropic hormone (ACTH) producing cells showed a weaker immunoreactivity, a decrease in cell number and a reduction of volume density when compared with normal larvae. In conclusion, the results obtained indicate a possible role for CRF in Bufo arenarum metamorphosis. CRF may regulate interrenal and thyroid activity by acting directly upon TSH and ACTH cells. On the other hand, TRH, GnRH and GHRH were inactive in stimulating growth or metamorphosis of Bufo arenarum. (C) 2000 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
Autor:Miranda, L.A.; Affanni, J.M.; Paz, D.A.
Filiación:Inst. Tecn. de Chascomús, 7130 Chascomús, Provincia de Buenos Aires, Argentina
Instituto de Neurociencia, Departamento de Biología, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Inst. Tecn. de Chascomús, Camino de Circunvalacion Laguna, Km 6. Cc 164, 7130 Chascomús, Provincia de Buenos Aires, Argentina
Palabras clave:Animalia; Anura; Bufo arenarum; Bufo arenarum; Mammalia; Ovis; Vertebrata
Año:2000
Volumen:286
Número:5
Página de inicio:473
Página de fin:480
DOI: http://dx.doi.org/10.1002/(SICI)1097-010X(20000401)286:5<473::AID-JEZ4>3.0.CO;2-H
Título revista:Journal of Experimental Zoology
Título revista abreviado:J. Exp. Zool.
ISSN:0022104X
CODEN:JEZOA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0022104X_v286_n5_p473_Miranda

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Citas:

---------- APA ----------
Miranda, L.A., Affanni, J.M. & Paz, D.A. (2000) . Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells. Journal of Experimental Zoology, 286(5), 473-480.
http://dx.doi.org/10.1002/(SICI)1097-010X(20000401)286:5<473::AID-JEZ4>3.0.CO;2-H
---------- CHICAGO ----------
Miranda, L.A., Affanni, J.M., Paz, D.A. "Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells" . Journal of Experimental Zoology 286, no. 5 (2000) : 473-480.
http://dx.doi.org/10.1002/(SICI)1097-010X(20000401)286:5<473::AID-JEZ4>3.0.CO;2-H
---------- MLA ----------
Miranda, L.A., Affanni, J.M., Paz, D.A. "Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells" . Journal of Experimental Zoology, vol. 286, no. 5, 2000, pp. 473-480.
http://dx.doi.org/10.1002/(SICI)1097-010X(20000401)286:5<473::AID-JEZ4>3.0.CO;2-H
---------- VANCOUVER ----------
Miranda, L.A., Affanni, J.M., Paz, D.A. Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells. J. Exp. Zool. 2000;286(5):473-480.
http://dx.doi.org/10.1002/(SICI)1097-010X(20000401)286:5<473::AID-JEZ4>3.0.CO;2-H