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Abstract:

The green turtle, Chelonia mydas, like other species of marine turtles, shows great migratory displacement between its nesting and feeding grounds. In an attempt to characterize the southernmost feeding grounds of this species, mtDNA sequence variation of green turtle aggregations in Argentinean waters was studied to elucidate genetic variation and infer possible origins. The goal of the present study is contemplated within the main purpose of the PRICTMA (Regional Program for Sea Turtle Research in Conservation of Argentina) and the Network ASO-Tortugas (Red Atlántico Sur Occidental-Tortugas) which are dedicated to promoting conservation studies in marine turtles in the region. A 486-bp fragment of the mitochondrial DNA control region was sequenced from 93 samples of incidentally caught juveniles from 2004 to 2007, revealing 9 haplotypes. Nucleotide and haplotype diversity were similar to those detected in other Brazilian feeding grounds (Ubatuba and Atol das Rocas/Fernando de Noronha). Analysis of molecular variance (AMOVA) indicated significant genetic differentiation among 9 western Atlantic feeding grounds for which data is currently available, suggesting variable contributions from different nesting colonies (F ST=0.29, P<10 -4; Φ ST=0.55, P<10 -4). Mitochondrial DNA haplotype distributions revealed significant heterogeneity among feeding grounds (X 2: 804.84, P<10 -4). A pairwise analysis revealed that most western Atlantic feeding grounds are genetically differentiated. The weighted and unweighted mixed stock analyses suggests that green turtles at Argentinean feeding grounds originate mainly in the Ascension Island rookery, with less contribution from rookeries in Suriname, Aves Island and Trindade Island.The present results improve our knowledge of the population structure and migration patterns of the Atlantic green turtle, and inform conservation measures on feeding grounds, which may be thousands of kilometers away from the nesting colonies. This information is required to further government efforts for this endangered species. © 2011 Elsevier B.V.

Registro:

Documento: Artículo
Título:Genetic composition of green turtle feeding grounds in coastal waters of Argentina based on mitochondrial DNA
Autor:Prosdocimi, L.; González Carman, V.; Albareda, D.A.; Remis, M.I.
Filiación:Laboratorio Genética de Poblaciones, Dpto. Ecología, Genética y Evolución, FCEN, Universidad de Buenos Aires, Intendente Güiraldes 2160, C1428EGA Capital Federal, Buenos Aires, Argentina
Fundación Mundo Marino-PRICTMA, Buenos Aires, Argentina
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP) CONICET, Mar del Plata, Buenos Aires, Argentina
Aquamarina-CECIM-PRICTMA, Buenos Aires, Argentina
Acuario del Jardín Zoológico de Buenos Aires-PRICTMA, Buenos Aires, Argentina
Palabras clave:Chelonia mydas; Endangered species; Feeding ground; Genetic diversity; Mitochondrial DNA; Population structure; coastal water; conservation genetics; conservation management; endangered species; feeding ground; genetic differentiation; genetic marker; genetic structure; genotype; migratory behavior; mitochondrial DNA; nest site; population structure; species conservation; state role; turtle; variance analysis; Argentina; Ascension Island; Atlantic Ocean; Atlantic Ocean (West); Suriname; Trindade; Aves; Chelonia mydas; Cheloniidae; Testudines
Año:2012
Volumen:412
Página de inicio:37
Página de fin:45
DOI: http://dx.doi.org/10.1016/j.jembe.2011.10.015
Título revista:Journal of Experimental Marine Biology and Ecology
Título revista abreviado:J. Exp. Mar. Biol. Ecol.
ISSN:00220981
CODEN:JEMBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220981_v412_n_p37_Prosdocimi

Referencias:

  • Allard, M.W., Miyamoto, M.M., Bjorndal, K.A., Bolten, A.B., Bowen, B.W., Support for natal homing in green turtles from mitochondrial DNA sequences (1994) Copeia, 1, pp. 34-41
  • Avise, J.C., (2004) Molecular Markers Natural History and Evolution, , Sinauer Associates, Inc., Sunderland M.A., USA
  • Bagley, D.A., Characterizing juvenile green turtles (Chelonia mydas) from three east central Florida Developmental habitats. (2003) MS Thesis., , University of Central Florida, Orlando, FL
  • Bandelt, H.J., Forster, P., Röhl, A., Median-joining networks for inferring intraspecific phylogenies (1999) Mol. Biol. Evol., 16, pp. 37-48
  • Bass, A.L., Witzell, W.N., Demographic composition of immature green turtles (Chelonia mydas) from the east central Florida coast, evidence from mtDNA markers (2000) Herpetologica, 56, pp. 357-367
  • Bass, A.L., Lagueux, C.J., Bowen, B.W., Origin of green turtles. Chelonia mydas at "sleeping rocks" of the northeast of Nicaragua (1998) Copeia, 4, pp. 1064-1069
  • Bass, A.L., Epperly, S.P., Braun-McNeill, J., Multi-year analysis of stock composition of loggerhead turtle (Caretta caretta) foraging habitat using maximum likelihood and Bayesian methods (2004) Conserv. Genet., 5, pp. 783-796
  • Bass, A.L., Epperly, S.P., Braun-McNeill, J., Green turtle (Chelonia mydas) foraging and nesting aggregations in the Caribbean and Atlantic, impact of currents and behavior on dispersal (2006) J. Hered., 97, pp. 346-354
  • Bellini, C., Sanches, T.M., Reproduction and feeding of marine turtles in the Fernando de Noronha Archipelago, Brazil (1996) Mar. Turt. Newsl., 74, pp. 12-13
  • Bellini, C., Marcovaldi, M.Â., Sanches, T.M., Grossman, A., Sales, G., Atol Das Rocas Biological Reserve: second largest Chelonia rookery in Brazil (1996) Mar. Turt. Newsl., 72, pp. 1-2
  • Bjorndal, K.A., Bolten, A.B., Annual variation in source contributions to mixed stock, implications for quantifying connectivity (2008) Mol. Ecol., 17, pp. 2185-2193
  • Bjorndal, K.A., Bolten, A.B., Troeng, S., Population structure and genetic diversity in green turtles nesting at Tortuguero, Costa Rica, based on mitochondrial DNA control region sequences (2005) Mar. Biol., 147, pp. 1449-1457
  • Bjorndal, K.A., Bolten, A.B., Moreira, L., Bellini, C., Marcovaldi, M.A., Population structure and diversity of Brazilian green turtle rookeries based on mitochondrial DNA sequences (2006) Chelonia Conserv. Biol., 5 (2), pp. 262-268
  • Bolker, B.M., Okuyama, T., Bjorndal, K.A., Bolten, A.B., Incorporating multiple mixed stocks in mixed stock analysis, 'many-to-many' analyses (2007) Mol. Ecol., 16, pp. 685-695
  • Bolten, A.B., Techniques for measuring sea turtles (1999) Research and Management Techniques for the Conservation of Sea Turtles, pp. 110-114. , IUCN/SSC Marine Turtle Specialist Group Publication, K.L. Eckert, K.A. Bjorndal, F.A. Abreu-Grobois, M. Donnelly (Eds.)
  • Bolten, A.B., Active swimmers-passive drifters: The oceanic juvenile stage of loggerheads in the Atlantic Ocean (2003) Loggerhead Sea Turtles, pp. 63-78. , Smithsonian Books, Washington, DC, USA, A.B. Bolten, B.E. Witherington (Eds.)
  • Bolten, A.B., Variation in sea turtle life history patterns: neritic versus oceanic developmental stages (2003) The Biology of Sea Turtles, 2, pp. 243-457. , CRC Press, Boca Raton, FL, 257, P.L. Lutz, J.A. Musick, J. Wyneken (Eds.)
  • Boltovskoy, D., (1981) Atlas del zooplancton del Atlántico Sudoccidental y métodos de trabajo con el zooplancton marino, p. 936. , Publ. Especial INIDEP, Mar del Plata (Argentina)
  • Bourjea, J., Lapégue, S., Gagnevin, L., Broderick, D., Mortimer, J., Ciccione, S., Roos, D., Grizel, H., Phylogeography of the green turtle, Chelonia mydas, in the Southwest Indian Ocean (2007) Mol. Ecol., 16, pp. 175-186
  • Bowen, B.W., Meylan, A.B., Ross, J.P., Limpus, C.J., Balazs, G.H., Avise, J.C., Global population structure and natural history of the green turtle (Chelonia mydas) in terms of matriarchal phylogeny (1992) Evolution, 46, pp. 865-881
  • Bowen, B.W., Bass, A.L., Chow, S.M., Bostrom, M., Bjorndal, K.A., Bolten, A.B., Okuyama, T., Lacasella, E., Natal homing in juvenile loggerhead turtles (Caretta caretta) (2004) Mol. Ecol., 13, pp. 3797-3808
  • Bowen, B.W., Bass, A.L., Soares, L., Toonen, R.J., Conservation implications of complex population structure, lessons from the loggerhead turtle (Caretta caretta) (2005) Mol. Ecol., 14, pp. 2389-2402
  • Broderick, A.C., Godley, B.J., Hays, G.C., Trophic status drives inter-annual variability in nesting numbers of marine turtles (2001) Proc. R. Soc. Lond. B, 268, pp. 1481-1487
  • Dutton, P.H., Methods for collection and preservation of samples for sea turtle genetic studies (1996) Proceedings of the International Symposium on Sea Turtles Conservation Genetics, NOAA Technical Memorandum NMFS-SEFSC, Miami (FL), 396, pp. 17-24. , B.W. Bowen, W.N. Witzell (Eds.)
  • Encalada, S.E., Lahanas, P.N., Bjorndal, K.A., Bolten, A.B., Miyamoto, M.M., Bowen, B.W., Phylogeography and population structure of the Atlantic and Mediterranean green turtle Chelonia mydas, a mitochondrial DNA control region sequence assessment (1996) Mol. Ecol., 4, pp. 473-483
  • Epperly, S.P., Braun, J., Veishlow, A., Sea turtles in North Carolina waters (1995) Conserv. Biol., 9, pp. 384-394
  • Excoffier, L., Laval, G., Schneider, S., Arlequin version 3.0, an integrated software package for population genetics data analysis (2005) Evol. Bioinform. Online, 1, pp. 47-50
  • FitzSimmons, N.N., Limpus, C.J., Norman, J.A., Goldizen, A.R., Miller, J.D., Moritz, C., Philopatry of male marine turtle inferred from mitochondrial DNA markers (1997) Proc. Natl. Acad. Sci. U. S. A., 94, pp. 8912-8917
  • FitzSimmons, N.N., Moritz, C., Limpus, C.J., Pope, L., Prince, R., Geographic structure of mitochondrial and nuclear gene polymorphisms in Australian green turtle populations and male-biased gene flow (1997) Genetics, 147, pp. 1843-1854
  • Formia, A., Population and genetic structure of the green turtle (Chelonia mydas) in West and Central Africa (2002), Implications for management and Conservation (dissertation). Cardiff (UK) - Cardiff University; Formia, A., Godley, B.J., Dontaine, J.F., Bruford, M.W., Mitochondrial DNA diversity and phylogeography of endangered green turtle (Chelonia mydas) populations in Africa (2006) Conserv. Genet., 7, pp. 353-369
  • Formia, A., Broderick, A.C., Glen, F., Godley, B.J., Hays, G.C., Bruford, W., Genetic composition of the Ascension Island green turtle rookery based on mitochondrial DNA, implications for sampling and diversity (2007) Endanger. Species Res., 3, pp. 145-158
  • Frankham, R., Balou, J.D., Briscoe, D.A., (2002) Introduction to Conservation Genetics, , Cambridge University Press, UK
  • Godley, B.J., Broderick, A.C., Hays, G.C., Nesting of green turtles (Chelonia mydas) at Ascension Island, South Atlantic (2001) Biol. Conserv., 97, pp. 151-158
  • González Carman, V., Álvarez, K., Prosdocimi, L., Inchaurraga, M.C., Dellacasa, R., Faiella, A., Echenique, C., Mianzan, H., Temperate SW Atlantic: a feeding and developmental habitat for endangered sea turtles (2011) Mar. Biol. Res., 7, pp. 500-508
  • Guerrero, R.A., Oceanografía física del estuario de Río de la Plata y el sistema costero de El Rincón (1998) INIDEP Informe Técnico, Mar del Plata, Argentina, 21, pp. 29-54. , C. Lasta (Ed.) Resultados de una campaña de evaluación de recursos demersales costeros de la Provincia de Buenos Aires y del litoral uruguayo
  • Hall, T.A., BioEdit, a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symposium Series, pp. 95-98
  • Heppel, S.S., Snover, M.L., Crowder, L.B., Sea turtle population ecology (2003) The Biology of Sea Turtle, pp. 275-306. , CRC Press, Boca Ratón (FL), P.L. Lutz, J.A. Musick, J. Wyneken (Eds.)
  • Higgins, D.G., Sharp, P.M., CLUSTAL, A package for performing multiple sequence alignment on a microcomputer (1988) Gene, 73, pp. 237-244
  • Hudson, R.R., Boos, D.D., Kaplan, N.L., A statistical test for detecting geographic subdivision (1992) Mol. Biol. Evol., 9, pp. 138-151
  • (2009) IUCN 2009: IUCN Red List of Threatened Species. Version 2009.2, , http://www.iucnredlist.org, IUCN
  • Kamezaki, N., Matsui, M., Geographic variation in skull morphology of the green turtle, Chelonia mydas, with a taxonomic discussion (1995) J. Herpetol., 29, pp. 51-60
  • Karl, S.A., Bowen, B.W., Avise, J.C., Global population structure and male mediated gene flow in the green turtle (Chelonia mydas), RFLP analyses of anonymous nuclear DNA regions (1992) Genetics, 31, pp. 163-173
  • Kaska, Y., Genetic structure of Mediterranean sea turtle populations (2000) Turk. J. Zool., 24, pp. 191-197
  • Kimura, M., A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences (1980) J. Mol. Evol., 16, pp. 111-120
  • Lahanas, P.N., Miyamoto, M.M., Bjorndal, K.A., Bolten, A.B., Molecular evolution and population genetics of Greater Caribbean green turtles (Chelonia mydas) as inferred from mitochondrial DNA control region sequences (1994) Genetica, 94, pp. 57-67
  • Lahanas, P.N., Bjorndal, K.A., Bolten, A.B., Encalada, S.E., Miyamoto, M.M., Valverde, R.A., Bowen, B.W., Genetic composition of green turtle (Chelonia mydas) feeding grounds population, evidence for multiple origins (1998) Mar. Biol., 130, pp. 345-352
  • López-Mendilaharsu, M., Estrades, A., Caraccio, M.N., Calvo, V., Hernández, M., Quirici, V., Biología, ecología y etología de las tortugas marinas en la zona costera Uruguaya (2006) Bases para la conservación y el manejo de la costa Uruguaya, Montevideo, pp. 247-258. , R. Menafra, L. Rodríguez-Gallego, F. Scarabino, D. Conde (Eds.)
  • Luke, K., Horrockas, J.A., Le Roux, R.A., Dutton, P.H., Origins of green turtle (Chelonia mydas) feeding aggregations around Barbados, West Indies (2004) Mar. Biol., 144, pp. 799-805
  • McElroy, D., Moran, P., Bermingham, E., Kornfield, I., (1991) REAP. The Restriction Enzyme Analyses Package. Version 4.0. Department of Zoology, , Migratory Fish Research Institute and Center for Marine Studies, University of Maine, Orono, USA
  • Mianzán, H.W., Lasta, C.A., Acha, E.M., Guerrero, R.A., Macchi, G.J., Bremec, C., The Río de la Plata estuary Argentina - Uruguay (2001) Ecological Studies: Coastal Marine Ecosystems of Latin America, pp. 186-204. , Springer-Verlag, Berlin, U. Seeliger, L.D. De Lacerda, B. Kjerfve (Eds.)
  • Moritz, C., Defining "Evolutionarily Significant Units" for conservation (1994) Trends Ecol. Evol., 9, pp. 373-375
  • Musick, J.A., Limpus, C.J., Habitat utilization and migration in juvenile sea turtles (1997) The Biology of Sea Turtles, pp. 137-163. , CRC Press, Boca Raton, Florida, P. Lutz, J.A. Musick (Eds.)
  • Naro-Maciel, E., Becker, J.H., Lima, E.H.S.M., Marcov, M.A., Desalle, R., Testing dispersal hypotheses in foraging green sea turtles (Chelonia mydas) of Brazil (2007) J. Hered., 98, pp. 29-39
  • Nei, M., (1987) Molecular Evolutionary Genetics, , Columbia University Press, New York (NY)
  • Norman, J.A., Moritz, C., Limpus, C.J., Mitochondrial DNA control region polymorphisms, genetic markers for ecological studies of marine turtles (1994) Mol. Ecol., 3, pp. 363-373
  • Peakall, R., Smouse, P.E., GENALEX 6, genetic analysis in Excel. Population genetic software for teaching and research (2006) Mol. Ecol. Notes, 6, pp. 288-295
  • Pella, J.J., Masuda, M., Bayesian methods for analysis of stock mixtures from genetic characters (2001) Fish. Bull., 9, pp. 151-167
  • Pella, J.J., Milner, G.B., Use of genetic markers in stock composition analysis (1987) Population genetics and fishery management, pp. 247-276. , University of Washington Press, Seattle (WA), N. Ryman, F. Utter (Eds.)
  • Piola, A.R., Matano, R.P., Brazil and Falklands (Malvinas) Currents (2001) Encyclopedia of Ocean Sciences, pp. 340-349. , Academic Press, Londres, J.H. Steele, S.A. Thorpe, K.K. Turekian (Eds.)
  • Plotkin, P., Adult migrations and habitat use (2003) The Biology of Sea Turtles, pp. 225-242. , CRC Press, Boca Raton, FL, P.L. Lutz, J.A. Musick, J. Wyneken (Eds.)
  • Roberts, M.A., Schwartz, T.S., Karl, S.A., Global population genetic structure and male-mediated gene flow in the green sea turtle (Chelonia mydas), analysis of microsatellite loci (2004) Genetics, 166, pp. 1857-1870
  • Roff, D.A., Bentzen, P., The statistical analysis of mitochondrial DNA polymorphisms, X 2 and the problem of small samples (1989) Mol. Biol. Evol., 6 (5), pp. 539-545
  • Schulz, J.P., Sea turtles nesting in Suriname (1975) Zool. Verh. (Leiden), 143, pp. 3-172
  • Seminoff, J.A., (2002) IUCN Red List Status Assessment Green Turtle (Chelonia mydas), , Marine Turtle Specialist Group, The World Conservation Union IUCN, Gland (Switzerland)
  • Seminoff, J.A., (2004) Global Status Assessment Green Turtle (Chelonia mydas), , Marine Turtle Specialist Group, The World Conservation Union IUCN, Gland (Switzerland)
  • Seminoff, J.A., Abreu-Grobois, F.A., Assessing the global status of sea turtles under the IUCN globally distributed species criteria: assumptions, caveats, limitations, and a call for more demographic data (2007) Proceedings of the Twenty-fourth Annual Symposium on Sea Turtle Biology and Conservation, 567, pp. 34-35. , NMFS-SEFSC, San Jose, Costa Rica, R.B. Mast, B.J. Hutchinson, A.H. Hutchinson (Eds.)
  • (1996) Statistica 5 for Windows (Computer Program Manual), , Tulsa OK, Statistica Statsoft Inc
  • Tomczak, M., Godfrey, J.S., (2003) Regional Oceanography: An Introduction, pp. 229-252. , Daya Publishing House, Delhi, Chapter 14
  • Witham, R.M., The "Lost Years" question in young sea turtles (1998) Am. Zool., 20, pp. 525-530

Citas:

---------- APA ----------
Prosdocimi, L., González Carman, V., Albareda, D.A. & Remis, M.I. (2012) . Genetic composition of green turtle feeding grounds in coastal waters of Argentina based on mitochondrial DNA. Journal of Experimental Marine Biology and Ecology, 412, 37-45.
http://dx.doi.org/10.1016/j.jembe.2011.10.015
---------- CHICAGO ----------
Prosdocimi, L., González Carman, V., Albareda, D.A., Remis, M.I. "Genetic composition of green turtle feeding grounds in coastal waters of Argentina based on mitochondrial DNA" . Journal of Experimental Marine Biology and Ecology 412 (2012) : 37-45.
http://dx.doi.org/10.1016/j.jembe.2011.10.015
---------- MLA ----------
Prosdocimi, L., González Carman, V., Albareda, D.A., Remis, M.I. "Genetic composition of green turtle feeding grounds in coastal waters of Argentina based on mitochondrial DNA" . Journal of Experimental Marine Biology and Ecology, vol. 412, 2012, pp. 37-45.
http://dx.doi.org/10.1016/j.jembe.2011.10.015
---------- VANCOUVER ----------
Prosdocimi, L., González Carman, V., Albareda, D.A., Remis, M.I. Genetic composition of green turtle feeding grounds in coastal waters of Argentina based on mitochondrial DNA. J. Exp. Mar. Biol. Ecol. 2012;412:37-45.
http://dx.doi.org/10.1016/j.jembe.2011.10.015