Artículo

Bartolotta, S.A.; Scuteri, M.A.; Hick, A.S.; Palermo, J.; Rodriguez Brasco, M.F.; Hajdu, E.; Mothes, B.; Lerner, C.; Campos, M.; Carballo, M.A. "Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea" (2009) Journal of Experimental Marine Biology and Ecology. 369(2):144-147
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Abstract:

Marine sponges are a rich source of structurally and biologically active metabolites of biomedical importance. We screened polar and non-polar samples of crude extracts obtained from marine sponges collected in different locations of Argentinean south sea coast, as a novel approach for their characterization. The evaluation was performed using cytotoxic and genotoxic biomarkers such as mitotic index (MI), cell proliferation kinetics (CPK) and sister chromatid exchanges (SCE), monitored in vitro using peripheral blood lymphocytes. Statistical analysis was performed using two-way analysis of variance (ANOVA). The extracts evaluated belonged to: Callyspongia flabellata (BURTON, 1932) (Callyspongiidae); Plicatellopsis sp.(Suberitidae); Callyspongia fortis (RIDLEY, 1881) (Callyspongiidae); Clathria (Microciona) antarctica (TOPSENT, 1917) (Microcionidae); Spongia (Spongia) magellanica (THIELE, 1905) (Spongiidae); Halicnemia papillosa (THIELE, 1905) (Axinellidae); Cliona chilensis (THIELE, 1905) (Clionidae); Haliclona sp. 1; Haliclona sp. 2(Chalinidae). Genotoxicity studies revealed that the evaluated sponge extracts did not exhibit cytotoxic activity measured from mitotic index MI and cell proliferation kinetics(CPK). In contrast, sister chromatid exchanges (SCE) showed that the non-polar extract of Callyspongia fortis and the polar extract of Cliona chilensis presented significant differences in SCE frequency (p < 0.001), when compared with control cultures. These results emphasize the need to set up a standard battery of "in vitro" genotoxicity testing for new chemicals, pharmaceutical and drugs. © 2008 Elsevier B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea
Autor:Bartolotta, S.A.; Scuteri, M.A.; Hick, A.S.; Palermo, J.; Rodriguez Brasco, M.F.; Hajdu, E.; Mothes, B.; Lerner, C.; Campos, M.; Carballo, M.A.
Filiación:GIGETOX, Citogenética Humana y Genética Toxicológica, INFIBIOC, Junín 956, (1113), Buenos Aires, Argentina
Departamento de Tecnología, Universidad Nacional de Luján, Ruta 5 y 7. , Luján, 6700 Buenos Aires, Argentina
UMYMFOR - Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
Museu Nacional, UFRJ, Quinta da Boa Vista, s/n, 20940-040, Rio de Janeiro, RJ, Brazil
Laboratório de Poríferos Marinhos, Museu de Ciências Naturais Fundação Zoobotânica do Rio Grande do Sul, Brazil. Rua Salvador Franca 1427, 90690-000 Porto Alegre, RS, Brazil
Palabras clave:Cell proliferation kinetics; Cytotoxicity; Genotoxicity; Marine sponges; Mitotic index; Sister chromatid exchange; animal product; biomarker; drug; genotoxicity; marine ecosystem; metabolite; sponge; toxicity test; variance analysis; Axinellidae; Callyspongia; Callyspongiidae; Chalinidae; Clathria; Cliona; Haliclona; Haliclona sp.; Halicnemia; Microciona; Microcionidae; Spongia; Spongiidae; Suberitidae
Año:2009
Volumen:369
Número:2
Página de inicio:144
Página de fin:147
DOI: http://dx.doi.org/10.1016/j.jembe.2008.11.008
Título revista:Journal of Experimental Marine Biology and Ecology
Título revista abreviado:J. Exp. Mar. Biol. Ecol.
ISSN:00220981
CODEN:JEMBA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220981_v369_n2_p144_Bartolotta

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Citas:

---------- APA ----------
Bartolotta, S.A., Scuteri, M.A., Hick, A.S., Palermo, J., Rodriguez Brasco, M.F., Hajdu, E., Mothes, B.,..., Carballo, M.A. (2009) . Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea. Journal of Experimental Marine Biology and Ecology, 369(2), 144-147.
http://dx.doi.org/10.1016/j.jembe.2008.11.008
---------- CHICAGO ----------
Bartolotta, S.A., Scuteri, M.A., Hick, A.S., Palermo, J., Rodriguez Brasco, M.F., Hajdu, E., et al. "Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea" . Journal of Experimental Marine Biology and Ecology 369, no. 2 (2009) : 144-147.
http://dx.doi.org/10.1016/j.jembe.2008.11.008
---------- MLA ----------
Bartolotta, S.A., Scuteri, M.A., Hick, A.S., Palermo, J., Rodriguez Brasco, M.F., Hajdu, E., et al. "Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea" . Journal of Experimental Marine Biology and Ecology, vol. 369, no. 2, 2009, pp. 144-147.
http://dx.doi.org/10.1016/j.jembe.2008.11.008
---------- VANCOUVER ----------
Bartolotta, S.A., Scuteri, M.A., Hick, A.S., Palermo, J., Rodriguez Brasco, M.F., Hajdu, E., et al. Evaluation of genotoxic biomarkers in extracts of marine sponges from Argentinean South Sea. J. Exp. Mar. Biol. Ecol. 2009;369(2):144-147.
http://dx.doi.org/10.1016/j.jembe.2008.11.008