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Abstract:

Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical (O2·-). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues. © 2012 The Author.

Registro:

Documento: Artículo
Título:Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
Autor:Roqueiro, G.; Maldonado, S.; Ríos, M.D.C.; Maroder, H.
Filiación:Instituto de Recursos Biológicos, INTA-Castelar, B1712WAA Hurlingham, Argentina
Departamento de Ciencias Básicas, Universidad Nacional de Luján, 6700 Luján, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina
Palabras clave:Antioxidant enzymes; lipid peroxidation; normal germination; oxidative burst; priming; reactive oxygen species (ROS); reactive oxygen metabolite; vegetable protein; water; article; germination; growth, development and aging; metabolism; oxidative stress; plant seed; willow; Germination; Oxidative Stress; Plant Proteins; Reactive Oxygen Species; Salix; Seeds; Water; Salix nigra
Año:2012
Volumen:63
Número:10
Página de inicio:3631
Página de fin:3642
DOI: http://dx.doi.org/10.1093/jxb/ers030
Título revista:Journal of Experimental Botany
Título revista abreviado:J. Exp. Bot.
ISSN:00220957
CODEN:JEBOA
CAS:water, 7732-18-5; Plant Proteins; Reactive Oxygen Species; Water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v63_n10_p3631_Roqueiro

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Citas:

---------- APA ----------
Roqueiro, G., Maldonado, S., Ríos, M.D.C. & Maroder, H. (2012) . Fluctuation of oxidative stress indicators in Salix nigra seeds during priming. Journal of Experimental Botany, 63(10), 3631-3642.
http://dx.doi.org/10.1093/jxb/ers030
---------- CHICAGO ----------
Roqueiro, G., Maldonado, S., Ríos, M.D.C., Maroder, H. "Fluctuation of oxidative stress indicators in Salix nigra seeds during priming" . Journal of Experimental Botany 63, no. 10 (2012) : 3631-3642.
http://dx.doi.org/10.1093/jxb/ers030
---------- MLA ----------
Roqueiro, G., Maldonado, S., Ríos, M.D.C., Maroder, H. "Fluctuation of oxidative stress indicators in Salix nigra seeds during priming" . Journal of Experimental Botany, vol. 63, no. 10, 2012, pp. 3631-3642.
http://dx.doi.org/10.1093/jxb/ers030
---------- VANCOUVER ----------
Roqueiro, G., Maldonado, S., Ríos, M.D.C., Maroder, H. Fluctuation of oxidative stress indicators in Salix nigra seeds during priming. J. Exp. Bot. 2012;63(10):3631-3642.
http://dx.doi.org/10.1093/jxb/ers030