Abstract:
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when in vitro-cultured potato plants were transferred to high sucrose or high sorbitol containing media. Glucose, fructose or a mixture of both showed no effect on CDPK expression. Okadaic acid blocked sucrose-inducible gene expression, suggesting that phosphatases from the PP1/PP2A family could also participate in the regulation of StCDPK1 and other tuberization-related genes.
Registro:
Documento: |
Artículo
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Título: | StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
Autor: | Raíces, M.; Ulloa, R.M.; MacIntosh, G.C.; Crespi, M.; Téllez-Iñón, M.T. |
Filiación: | Inst. Invest. Ing. Genet. y Biol. M., CONICET, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Delaware Biotechnology Institute, 15 Innovation Way, Newark, DE 19711, United States Inst. des Sci. Vég., CNRS, 1 Avenue de la Terrasse, F-91, 198 Gif sur Yvette, France
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Palabras clave: | Gene expression; Okadaic acid; Potato stolons; StCDPK1; Sucrose; Enzymes; Organic acids; Plants (botany); RNA; Sugar (sucrose); Swelling; Hybridization; Genetic engineering; calcium binding protein; CDPK1 protein, plant; okadaic acid; protein kinase; sucrose; vegetable protein; article; drug effect; enzymology; gene expression regulation; genetics; kinetics; photoperiodicity; potato; Calcium-Binding Proteins; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Kinetics; Okadaic Acid; Photoperiod; Plant Proteins; Protein Kinases; Solanum tuberosum; Sucrose; Embryophyta; Eukaryota; Solanum; Solanum tuberosum |
Año: | 2003
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Volumen: | 54
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Número: | 392
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Página de inicio: | 2589
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Página de fin: | 2591
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DOI: |
http://dx.doi.org/10.1093/jxb/erg282 |
Título revista: | Journal of Experimental Botany
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Título revista abreviado: | J. Exp. Bot.
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ISSN: | 00220957
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CODEN: | JEBOA
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CAS: | okadaic acid, 78111-17-8; protein kinase, 9026-43-1; sucrose, 122880-25-5, 57-50-1; Calcium-Binding Proteins; CDPK1 protein, plant; Okadaic Acid, 78111-17-8; Plant Proteins; Protein Kinases, EC 2.7.1.37; Sucrose, 57-50-1
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v54_n392_p2589_Raices |
Referencias:
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Citas:
---------- APA ----------
Raíces, M., Ulloa, R.M., MacIntosh, G.C., Crespi, M. & Téllez-Iñón, M.T.
(2003)
. StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration. Journal of Experimental Botany, 54(392), 2589-2591.
http://dx.doi.org/10.1093/jxb/erg282---------- CHICAGO ----------
Raíces, M., Ulloa, R.M., MacIntosh, G.C., Crespi, M., Téllez-Iñón, M.T.
"StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration"
. Journal of Experimental Botany 54, no. 392
(2003) : 2589-2591.
http://dx.doi.org/10.1093/jxb/erg282---------- MLA ----------
Raíces, M., Ulloa, R.M., MacIntosh, G.C., Crespi, M., Téllez-Iñón, M.T.
"StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration"
. Journal of Experimental Botany, vol. 54, no. 392, 2003, pp. 2589-2591.
http://dx.doi.org/10.1093/jxb/erg282---------- VANCOUVER ----------
Raíces, M., Ulloa, R.M., MacIntosh, G.C., Crespi, M., Téllez-Iñón, M.T. StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration. J. Exp. Bot. 2003;54(392):2589-2591.
http://dx.doi.org/10.1093/jxb/erg282