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Abstract:

The role of GAs in promoting seed germination is well known and experiments with seeds from different species have suggested the requirement of de novo synthesis of GAs upon imbibition for germination. There are also strong indications that the enhancement of GA synthesis is part of the mechanism through which environmental signals (i.e. light) induce germination. Since along the GA biosynthetic pathway, oxidation at C-20 carried out by GA 20-oxidases is thought to be a site of regulation, a cDNA clone encoding a GA 20-oxidase was isolated from embryos of sorghum (SbGA 20ox). Expression analysis of this gene in embryos within imbibed caryopses with low dormancy showed detectable amounts of the specific mRNA early upon incubation, increasing thereafter. In contrast, it remained barely detectable in embryos from dormant caryopses. Changes in endogenous GA4 levels were in agreement with those of SbGA 20ox mRNA, suggesting that GA production might be regulated differentially at the level of transcription of this gene. The expression of SbGA 20ox was enhanced in incubated embryos isolated from either type of caryopses, illustrating a physiological control exerted by the surrounding seed tissues on gene expression. The results also show that ABA leads to a suppression of transcription of this gene.

Registro:

Documento: Artículo
Título:Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination
Autor:Pérez-Flores, L.; Carrari, F.; Osuna-Fernández, R.; Rodríguez, M.V.; Enciso, S.; Stanelloni, R.; Sánchez, R.A.; Bottini, R.; Iusem, N.D.; Benech-Arnold, R.L.
Filiación:UAM-Iztapalapa, México, Mexico
Lab. de Fisiol. y Biol. Molecular, FCEyN Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias, UNAM, México, Mexico
IFEVA-Catedra de Cerealicultura, Facultad de Agronomía, Universidad de Buenos Aires/CONICET, Av. San Martín 4453, 1417 Buenos Aires, Argentina
Lab. Fisiología Vegetal, Departamento de Ciencias Naturales, Campus Universitario, 5800 Río Cuarto, Argentina
Catedra de Quim. Organ. y Biol., Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina
Palabras clave:Dormancy; GA 20-oxidase; Germination; Gibberellins; Sorghum; Biosynthesis; Enzymes; Genes; Tissue; Seed germination; Seed; Amino Acid Sequence; Blotting, Southern; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Mixed Function Oxygenases; Molecular Sequence Data; Plant Growth Regulators; Poaceae; RNA, Messenger; Seeds; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Embryophyta; Eukaryota
Año:2003
Volumen:54
Número:390
Página de inicio:2071
Página de fin:2079
DOI: http://dx.doi.org/10.1093/jxb/erg233
Título revista:Journal of Experimental Botany
Título revista abreviado:J. Exp. Bot.
ISSN:00220957
CODEN:JEBOA
CAS:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing), EC 1.14.11.-; Mixed Function Oxygenases, EC 1.-; Plant Growth Regulators; RNA, Messenger
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220957_v54_n390_p2071_PerezFlores.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v54_n390_p2071_PerezFlores

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Citas:

---------- APA ----------
Pérez-Flores, L., Carrari, F., Osuna-Fernández, R., Rodríguez, M.V., Enciso, S., Stanelloni, R., Sánchez, R.A.,..., Benech-Arnold, R.L. (2003) . Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination. Journal of Experimental Botany, 54(390), 2071-2079.
http://dx.doi.org/10.1093/jxb/erg233
---------- CHICAGO ----------
Pérez-Flores, L., Carrari, F., Osuna-Fernández, R., Rodríguez, M.V., Enciso, S., Stanelloni, R., et al. "Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination" . Journal of Experimental Botany 54, no. 390 (2003) : 2071-2079.
http://dx.doi.org/10.1093/jxb/erg233
---------- MLA ----------
Pérez-Flores, L., Carrari, F., Osuna-Fernández, R., Rodríguez, M.V., Enciso, S., Stanelloni, R., et al. "Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination" . Journal of Experimental Botany, vol. 54, no. 390, 2003, pp. 2071-2079.
http://dx.doi.org/10.1093/jxb/erg233
---------- VANCOUVER ----------
Pérez-Flores, L., Carrari, F., Osuna-Fernández, R., Rodríguez, M.V., Enciso, S., Stanelloni, R., et al. Expression analysis of a GA 20-oxidase in embryos from two sorghum lines with contrasting dormancy: Possible participation of this gene in the hormonal control of germination. J. Exp. Bot. 2003;54(390):2071-2079.
http://dx.doi.org/10.1093/jxb/erg233