Artículo
Abstract:
Protoplasts were successfully isolated from internodal callus tissues of both Oxalis glaucifolia and O. rhombeo-ovata when they were digested in a solution containing 0.1% (w/v) Macerozyme R-10, 0.5% (w/v) cellulase Onozuka R-10 and 0.3 mmol m-3 sucrose. Protoplasts proliferated to give cell colonies on Gamborg et al.'s B5 medium supplemented with 0.3 mmol m-3 mannitol, 0.5 mg dm-32, 4-D, and 2.0 mg dm-3 kinetin. Callus was produced upon transfer of cell colonies to Murashige and Skoog medium containing 2.0 mg dm-3 l-naphthaleneacetic acid (NAA) and 0.1 mg dm-3 kinetin for O. glaucifolia, or with 5.0 mg dm-3 NAA and 0.5 mg dm-3 6-benzylaminopurine, for O. rhombeo-ovata. Plants were regenerated from O. glaucifolia protoplasts on a medium containing 0.1 mg dm-3 NAA, 1.0 mg dm-3 kinetin and 1.0 mg dm-3 gibberellic acid, but only vascular nodules were differentiated by O. rhombeo-ovata protoplast-derived calli. © 1989 Oxford University Press.
Registro:
| Documento: | Artículo |
| Título: | Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america |
| Autor: | Ochatt, S.J.; Escandon, A.S.; Martinez, A.J. |
| Filiación: | Centre de Ecofisiologia Vegetal, (CEVEG, CONICET), Serrano 665, 1414 Buenos Aires, Argentina Centro de Estudios Farmacologicos y de Principios Naturales, (CEFAPRIN, CONICET), Serrano 665, 1414 Buenos Aires, Argentina |
| Palabras clave: | Oxalis spp; Plant regeneration; Protoplasts; Tissue culture |
| Año: | 1989 |
| Volumen: | 40 |
| Número: | 4 |
| Página de inicio: | 493 |
| Página de fin: | 496 |
| DOI: | http://dx.doi.org/10.1093/jxb/40.4.493 |
| Título revista: | Journal of Experimental Botany |
| Título revista abreviado: | J. Exp. Bot. |
| ISSN: | 00220957 |
| CODEN: | JEBOA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v40_n4_p493_Ochatt |
Citas:
---------- APA ----------
Ochatt, S.J., Escandon, A.S. & Martinez, A.J. (1989) . Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america. Journal of Experimental Botany, 40(4), 493-496.http://dx.doi.org/10.1093/jxb/40.4.493
---------- CHICAGO ----------
Ochatt, S.J., Escandon, A.S., Martinez, A.J. "Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america" . Journal of Experimental Botany 40, no. 4 (1989) : 493-496.http://dx.doi.org/10.1093/jxb/40.4.493
---------- MLA ----------
Ochatt, S.J., Escandon, A.S., Martinez, A.J. "Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america" . Journal of Experimental Botany, vol. 40, no. 4, 1989, pp. 493-496.http://dx.doi.org/10.1093/jxb/40.4.493
---------- VANCOUVER ----------
Ochatt, S.J., Escandon, A.S., Martinez, A.J. Isolation, culture, and plant regeneration of protoplasts of two shrubby Oxalis species from south america. J. Exp. Bot. 1989;40(4):493-496.http://dx.doi.org/10.1093/jxb/40.4.493
