Artículo
Abstract:
Envelope membranes were isolated from potato tuber amyloplast by a discontinuous sucrose density gradient and high speed centrifugation. These membranes catalyzed the transfer of [14C]glucose from UDP-[14C]glucose to endogenous sterol acceptors and, in turn, catalyzed the esterification of steryl glucosides with fatty acids from an endogenous acyl donor. The synthesis of steryl glucosides was stimulated in the presence of Triton. X-100, while formation of acyl steryl glucosides was inhibited by the detergent. However, in the presence of an added sterol acceptor and Triton X-100, the inhibition of acyl steryl glucoside synthesis was overcome by the addition of phosphatidylethanolamine. The enzyme involved in steryl glucoside formation was solubilized by treatment of the envelope membranes with 0.3% Triton X-100. The solubilized enzyme had an almost absolute requirement for sterol acceptors. © 1985 Oxford University Press.
Registro:
| Documento: | Artículo |
| Título: | UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers |
| Autor: | Catz, D.S.; Tandecarz, J.S.; Cardini, C.E. |
| Filiación: | Instituto de Investigaciones Bioquimicas 'Fundacion Campomar', Facultad de Ciencias Exactas y Naturales, Antonio Machado, (1405) Buenos Aires, Argentina |
| Palabras clave: | Amyloplast membrane; Solanum tuberosum; Sterol glucosylation; Steryl glucoside acylation |
| Año: | 1985 |
| Volumen: | 36 |
| Número: | 4 |
| Página de inicio: | 602 |
| Página de fin: | 609 |
| DOI: | http://dx.doi.org/10.1093/jxb/36.4.602 |
| Título revista: | Journal of Experimental Botany |
| Título revista abreviado: | J. Exp. Bot. |
| ISSN: | 00220957 |
| CODEN: | JEBOA |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220957_v36_n4_p602_Catz |
Citas:
---------- APA ----------
Catz, D.S., Tandecarz, J.S. & Cardini, C.E. (1985) . UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers. Journal of Experimental Botany, 36(4), 602-609.http://dx.doi.org/10.1093/jxb/36.4.602
---------- CHICAGO ----------
Catz, D.S., Tandecarz, J.S., Cardini, C.E. "UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers" . Journal of Experimental Botany 36, no. 4 (1985) : 602-609.http://dx.doi.org/10.1093/jxb/36.4.602
---------- MLA ----------
Catz, D.S., Tandecarz, J.S., Cardini, C.E. "UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers" . Journal of Experimental Botany, vol. 36, no. 4, 1985, pp. 602-609.http://dx.doi.org/10.1093/jxb/36.4.602
---------- VANCOUVER ----------
Catz, D.S., Tandecarz, J.S., Cardini, C.E. UDP-glucose: Sterol glucosyltransferase and a steryl glucoside acyltransferase activity in amyloplast membranes from potato tubers. J. Exp. Bot. 1985;36(4):602-609.http://dx.doi.org/10.1093/jxb/36.4.602
