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Abstract:

In insects, pre-adult stages of the life cycle are exposed to variation in temperature that may differ from that in adults. However, the genetic basis for adaptation to environmental temperature could be similar between the pre-adult and the adult stages of the life cycle. Here, we tested quantitative trait loci (QTL) for heat-stress survival in larvae of Drosophila melanogaster, with and without a mild-heat-stress pre-treatment. Two sets of recombinant inbred lines derived from lines artificially selected for high and low levels of knockdown resistance to high temperature in young flies were used as the mapping population. There was no apparent increase in heat-shock survival between heat-pretreated and non-pretreated larvae. There was a positive correlation between the two experimental conditions of heat-shock survival (with and without a heat pre-treatment) except for males from one set of lines. Several QTL were identified involving all three major chromosomes. Most QTL for larval thermotolerance overlapped with thermotolerance QTL identified in previous studies for adults, indicating that heat-stress resistance is not genetically independent between life cycle stages because of either linkage or pleiotropy. The sign of the effects of some QTL alleles differed both between the sexes and between life stages. © 2013. Published by The Company of Biologists Ltd.

Registro:

Documento: Artículo
Título:Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster
Autor:Sambucetti, P.; Scannapieco, A.C.; Loeschcke, V.; Norry, F.M.
Filiación:Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Ciudad Autónoma de, Buenos Aires C1428EGA, Argentina
IEGEBA (CONICET-UBA), C-1428-EGA Buenos Aires, Argentina
Department of Bioscience, Aarhus University, Ny Munkegade 114, Building 1540, DK-8000 Aarhus C, Denmark
Palabras clave:Candidate genes; Heat shock; Larval survival; QTL; Thermotolerance; analysis of variance; animal; article; candidate genes; Drosophila melanogaster; female; genetic recombination; genetics; growth, development and aging; heat shock; heat shock response; heat tolerance; inbreeding; larval survival; life cycle stage; male; physiology; quantitative trait locus; statistical model; candidate genes; heat shock; larval survival; QTL; thermotolerance; Analysis of Variance; Animals; Drosophila melanogaster; Female; Heat-Shock Response; Inbreeding; Life Cycle Stages; Likelihood Functions; Male; Quantitative Trait Loci; Recombination, Genetic
Año:2013
Volumen:216
Número:15
Página de inicio:2953
Página de fin:2959
DOI: http://dx.doi.org/10.1242/jeb.079830
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v216_n15_p2953_Sambucetti

Referencias:

  • Anderson, A.R., Collinge, J.E., Hoffmann, A.A., Kellett, M., McKechnie, S.W., Thermal tolerance trade-offs associated with the right arm of chromosome 3 and marked by the hsr-omega gene in Drosophila melanogaster (2003) Heredity, 90, pp. 195-202
  • Arias, L.N., Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M., Survival of heat stress with and without heat hardening in Drosophila melanogaster: Interactions with larval density (2012) J. Exp. Biol., 215, pp. 2220-2225
  • Bettencourt, B.R., Kim, I.Y., Hoffmann, A.A., Feder, M.E., Response to natural and laboratory selection at the Drosophila hsp70 genes (2002) Evolution, 56, pp. 1796-1801
  • Bowler, K., Terblanche, J.S., Insect thermal tolerance: What is the role of ontogeny, ageing and senescence? (2008) Biol. Rev. Camb. Philos. Soc., 83, pp. 339-355
  • Carhan, A., Reeve, S., Dee, C.T., Baines, R.A., Moffat, K.G., Mutation in slowmo causes defects in Drosophila larval locomotor behaviour (2004) Invert. Neurosci., 5, pp. 65-75
  • Dupuis, J., Siegmund, D., Statistical methods for mapping quantitative trait loci from a dense set of markers (1999) Genetics, 151, pp. 373-386
  • Ekengren, S., Hultmark, D., A family of Turandot-related genes in the humoral stress response of Drosophila (2001) Biochem. Biophys. Res. Commun., 284, pp. 998-1003
  • Feder, M.E., Hofmann, G.E., Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology (1999) Annu. Rev. Physiol., 61, pp. 243-282
  • Feder, M.E., Cartaño, N.V., Milos, L., Krebs, R.A., Lindquist, S.L., Effect of engineering Hsp70 copy number on Hsp70 expression and tolerance of ecologically relevant heat shock in larvae and pupae of Drosophila melanogaster (1996) J. Exp. Biol., 199, pp. 1837-1844
  • Feder, M.E., Blair, N., Figueras, H., Natural thermal stress and heatshock protein expression in Drosophila larvae and pupae (1997) Funct. Ecol., 11, pp. 90-100
  • Feder, M.E., Roberts, S.P., Bordelon, A.C., Molecular thermal telemetry of free-ranging adult Drosophila melanogaster (2000) Oecologia, 123, pp. 460-465
  • The FlyBase database of the Drosophila genome projects and community literature (2003) Nuc. Acid Res., 31, pp. 172-175. , FlyBase Consortium
  • Franks, S.J., Hoffmann, A.A., Genetics of climate change adaptation (2012) Annu. Rev. Genet., 46, pp. 185-208
  • Frydenberg, J., Hoffmann, A.A., Loeschcke, V., DNA sequence variation and latitudinal associations in hsp23, hsp26 and hsp27 from natural populations of Drosophila melanogaster (2003) Mol. Ecol., 12, pp. 2025-2032
  • Gibbs, A.G., Perkins, M.C., Markow, T.A., No place to hide: Microclimates of sonoran desert drosophila (2003) J. Therm. Biol., 28, pp. 353-362
  • Hercus, M.J., Berrigan, D., Blows, M.W., Magiafoglou, A., Hoffmann, A.A., Resistance to temperature extremes between and within life cycle stages in Drosophila serrata,D. Birchii and their hybrids: Intraspecific and interspecific comparisons (2000) Biol. J. Linn. Soc. Lond., 71, pp. 403-416
  • Hoffmann, A.A., Daborn, P.J., Towards genetic markers in animal populations in animal populations as biomonitors for human-induced environmental change (2007) Ecol. Lett., 10, pp. 63-76
  • Hoffmann, A.A., Parsons, P.A., (1991) Evolutionary Genetics and Environmental Stress, , Oxford: Science Publisher
  • Hoffmann, A.A., Willi, Y., Detecting genetic responses to environmental change (2008) Nat. Rev. Genet., 9, pp. 421-432
  • Hoffmann, A.A., Sørensen, J.G., Loeschcke, V., Adaptation of Drosophila to temperature extremes: Bringing together quantitative and molecular approaches (2003) J. Therm. Biol., 28, pp. 175-216
  • Krebs, R.A., Feder, M.E., Hsp70 and larval thermotolerance in Drosophila melanogaster: How much is enough and when is more too much? (1998) J. Insect Physiol., 44, pp. 1091-1101
  • Krebs, R.A., Loeschcke, V., Resistance to thermal stress in preadult Drosophila buzzatii: Variation among populations and changes in relative resistance across life stages (1995) Biol. J. Linn. Soc. Lond., 56, pp. 517-531
  • Leemans, R., Egger, B., Loop, T., Kammermeier, L., He, H., Hartmann, B., Certa, U., Reichert, H., Quantitative transcript imaging in normal and heat-shocked Drosophila embryos by using high-density oligonucleotide arrays (2000) Proc. Natl. Acad. Sci. USA, 97, pp. 12138-12143
  • Loeschcke, V., Krebs, R.A., Selection for heat-shock resistance in larval and adult Drosophila buzzatii: Comparing direct and indirect responses (1996) Evolution, 50, pp. 2354-2359
  • Loeschcke, V., Kristensen, T.N., Norry, F.M., Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster (2011) J. Insect Physiol., 57, pp. 1227-1231
  • Lynch, M., Walsh, B., (1998) Genetics and Analysis of Quantitative Traits, , Sunderland, MA: Sinauer Associates
  • Mackay, T.F.C., Quantitative trait loci in Drosophila (2001) Nat. Rev. Genet., 2, pp. 11-20
  • McColl, G., Hoffmann, A.A., McKechnie, S.W., Response of two heat shock genes to selection for knockdown heat resistance in Drosophila melanogaster (1996) Genetics, 143, pp. 1615-1627
  • McKechnie, S.W., Halford, M.M., McColl, G., Hoffmann, A.A., Both allelic variation and expression of nuclear and cytoplasmic transcripts of Hsr-omega are closely associated with thermal phenotype in Drosophila (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 2423-2428
  • Moehring, A.J., Mackay, T.F.C., The quantitative genetic basis of male mating behavior in Drosophila melanogaster (2004) Genetics, 167, pp. 1249-1263
  • Morgan, T.J., Mackay, T.F.C., Quantitative trait loci for thermotolerance phenotypes in Drosophila melanogaster (2006) Heredity, 96, pp. 232-242
  • Nielsen, M.M., Sørensen, J.G., Kruhøffer, M., Justesen, J., Loeschcke, V., Phototransduction genes are up-regulated in a global gene expression study of Drosophila melanogaster selected for heat resistance (2006) Cell Stress Chaperones, 11, pp. 325-333
  • Norry, F.M., Dahlgaard, J., Loeschcke, V., Quantitative trait loci affecting knockdown resistance to high temperature in Drosophila melanogaster (2004) Mol. Ecol., 13, pp. 3585-3594
  • Norry, F.M., Gomez, F.H., Loeschcke, V., Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster (2007) Mol. Ecol., 16, pp. 3274-3284
  • Norry, F.M., Sambucetti, P., Scannapieco, A.C., Gomez, F.H., Loeschcke, V., X-linked QTL for knockdown resistance to high temperature in Drosophila melanogaster (2007) Insect Mol. Biol., 16, pp. 509-513
  • Norry, F.M., Scannapieco, A.C., Sambucetti, P., Bertoli, C.I., Loeschcke, V., QTL for the thermotolerance effect of heat hardening, knockdown resistance to heat and chill-coma recovery in an intercontinental set of recombinant inbred lines of Drosophila melanogaster (2008) Mol. Ecol., 17, pp. 4570-4581
  • Norry, F.M., Larsen, P.F., Liu, Y., Loeschcke, V., Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster (2009) J. Insect Physiol., 55, pp. 1050-1057
  • Rako, L., Blacket, M.J., McKechnie, S.W., Hoffmann, A.A., Candidate genes and thermal phenotypes: Identifying ecologically important genetic variation for thermotolerance in the Australian Drosophila melanogaster cline (2007) Mol. Ecol., 16, pp. 2948-2957
  • Rand, D.M., Weinreich, D.M., Lerman, D., Folk, D., Gilchrist, G.W., Three selections are better than one: Clinal variation of thermal QTL from independent selection experiments in Drosophila (2010) Evolution, 64, pp. 2921-2934
  • Reusch, T.B.H., Wood, T.E., Molecular ecology of global change (2007) Mol. Ecol., 16, pp. 3973-3992
  • Sørensen, J.G., Nielsen, M.M., Kruhøffer, M., Justesen, J., Loeschcke, V., Full genome gene expression analysis of the heat stress response in Drosophila melanogaster (2005) Cell Stress Chaperones, 10, pp. 312-328
  • Sørensen, J.G., Nielsen, M.M., Loeschcke, V., Gene expression profile analysis of Drosophila melanogaster selected for resistance to environmental stressors (2007) J. Evol. Biol., 20, pp. 1624-1636
  • Takahashi, K.H., Okada, Y., Teramura, K., Genome-wide deficiency screen for the genomic regions responsible for heat resistance in Drosophila melanogaster (2011) BMC Genet, 12, p. 57
  • Walser, J.C., Chen, B., Feder, M.E., Heat-shock promoters: Targets for evolution by P transposable elements in Drosophila (2006) PLoS Genet, 2, pp. e165
  • Wang, S., Basten, C.J., Zeng, Z.B., (2010) Windows QTL Cartographer 2.5, , Raleigh, NC: Department of Statistics, North Carolina State University
  • Zeng, Z.B., Precision mapping of quantitative trait loci (1994) Genetics, 136, pp. 1457-1468

Citas:

---------- APA ----------
Sambucetti, P., Scannapieco, A.C., Loeschcke, V. & Norry, F.M. (2013) . Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. Journal of Experimental Biology, 216(15), 2953-2959.
http://dx.doi.org/10.1242/jeb.079830
---------- CHICAGO ----------
Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. "Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster" . Journal of Experimental Biology 216, no. 15 (2013) : 2953-2959.
http://dx.doi.org/10.1242/jeb.079830
---------- MLA ----------
Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. "Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster" . Journal of Experimental Biology, vol. 216, no. 15, 2013, pp. 2953-2959.
http://dx.doi.org/10.1242/jeb.079830
---------- VANCOUVER ----------
Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. Heat-stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster. J. Exp. Biol. 2013;216(15):2953-2959.
http://dx.doi.org/10.1242/jeb.079830