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Abstract:

Survival of a potentially lethal high temperature stress is a genetically variable thermal adaptation trait in many organisms. Organisms cope with heat stress by basal or induced thermoresistance. Here, we tested quantitative trait loci (QTL) for heat stress survival (HSS) in Drosophila melanogaster, with and without a cyclic heat-hardening pre-treatment, for flies that were reared at low (LD) or high (HD) density. Mapping populations were two panels of recombinant inbred lines (RIL), which were previously constructed from heat stress-selected stocks: RIL-D48 and RIL-SH2, derived from backcrosses to stocks of low and high heat resistance, respectively. HSS increased with heat hardening in both LD and HD flies. In addition, HSS increased consistently with density in non-hardened flies. There was a significant interaction between heat hardening and density effects in RIL-D48. Several QTL were significant for both density and hardening treatments. Many QTL overlapped with thermotolerance QTL identified for other traits in previous studies based on LD cultures only. However, three new QTL were found in HD only (cytological ranges: 12E-16F6; 30A3-34C2; 49C-50C). Previously found thermotolerance QTL were also significant for flies from HD cultures. © 2012. Published by The Company of Biologists Ltd.

Registro:

Documento: Artículo
Título:Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density
Autor:Arias, L.N.; Sambucetti, P.; Scannapieco, A.C.; Loeschcke, V.; Norry, F.M.
Filiación:Departamento de Ecología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C-1428-EHA Buenos Aires, Argentina
Department of Bioscience, Aarhus University, Ny Munkegade 114, DK-8000 Aarhus C, Denmark
Palabras clave:High temperature stress; Quantitative trait loci; Thermal resistance; Thermotolerance; adaptation; animal; article; Drosophila melanogaster; genetics; heat; larva; phenotype; physiological stress; physiology; quantitative trait locus; Adaptation, Physiological; Animals; Drosophila melanogaster; Hot Temperature; Larva; Phenotype; Quantitative Trait Loci; Stress, Physiological; Drosophila melanogaster
Año:2012
Volumen:215
Número:13
Página de inicio:2220
Página de fin:2225
DOI: http://dx.doi.org/10.1242/jeb.069831
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v215_n13_p2220_Arias.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v215_n13_p2220_Arias

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Citas:

---------- APA ----------
Arias, L.N., Sambucetti, P., Scannapieco, A.C., Loeschcke, V. & Norry, F.M. (2012) . Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density. Journal of Experimental Biology, 215(13), 2220-2225.
http://dx.doi.org/10.1242/jeb.069831
---------- CHICAGO ----------
Arias, L.N., Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. "Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density" . Journal of Experimental Biology 215, no. 13 (2012) : 2220-2225.
http://dx.doi.org/10.1242/jeb.069831
---------- MLA ----------
Arias, L.N., Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. "Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density" . Journal of Experimental Biology, vol. 215, no. 13, 2012, pp. 2220-2225.
http://dx.doi.org/10.1242/jeb.069831
---------- VANCOUVER ----------
Arias, L.N., Sambucetti, P., Scannapieco, A.C., Loeschcke, V., Norry, F.M. Survival of heat stress with and without heat hardening in Drosophila melanogaster:Interactions with larval density. J. Exp. Biol. 2012;215(13):2220-2225.
http://dx.doi.org/10.1242/jeb.069831