Artículo
Schilman, P.E.; Waters, J.S.; Harrison, J.F.; Lighton, J.R.B. "Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster" (2011) Journal of Experimental Biology. 214(8):1271-1275
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Abstract:
Insects in general, and Drosophila in particular, are much more capable of surviving anoxia than vertebrates, and the mechanisms involved are of considerable biomedical and ecological interest. Temperature is likely to strongly affect both the rates of damage occurring in anoxia and the recovery processes in normoxia, but as yet there is no information on the effect of this crucial variable on recovery rates from anoxia in any animal. We studied the effects of temperature, and thus indirectly of metabolic flux rates, on survival and recovery times of individual male Drosophila melanogaster following anoxia and O2 reperfusion. Individual flies were reared at 25° and exposed to an anoxic period of 7.5, 25, 42.5 or 60?min at 20, 25 or 30°. Before, during and after anoxic exposure the flies' metabolic rates (MRs), rates of water loss and activity indices were recorded. Temperature strongly affected the MR of the flies, with a Q10 of 2.21. Temperature did not affect the slope of the relationship between time to recovery and duration of anoxic exposure, suggesting that thermal effects on damage and repair rates were similar. However, the intercept of that relationship was significantly lower (i.e. recovery was most rapid) at 25°, which was the rearing temperature. When temperatures during exposure to anoxia and during recovery were switched, recovery times matched those predicted from a model in which the accumulation and clearance of metabolic end-products share a similar dependence on temperature. ©2011. Published by The Company of Biologists Ltd.
Registro:
| Documento: | Artículo |
| Título: | Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster |
| Autor: | Schilman, P.E.; Waters, J.S.; Harrison, J.F.; Lighton, J.R.B. |
| Filiación: | Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina Section of Organismal, Integrative, and Systems Biology, School of Life Sciences, Arizona State University, PO Box 874501, Tempe, AZ 85287-4501, United States Department of Biological Sciences, University of Nevada at Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4004, United States Sable Systems International, 6000 S. Eastern Blvd Bldg 1, Las Vegas, NV 89119, United States |
| Palabras clave: | Insect; Ischemia; O2 production; Reperfusion damage; Temperature; oxygen; animal; anoxia; article; Drosophila melanogaster; instrumentation; male; metabolism; methodology; photochemistry; physiology; spirometry; survival rate; temperature; Animals; Anoxia; Drosophila melanogaster; Male; Oxygen; Photochemistry; Spirometry; Survival Rate; Temperature; Animalia; Drosophila melanogaster; Hexapoda; Vertebrata |
| Año: | 2011 |
| Volumen: | 214 |
| Número: | 8 |
| Página de inicio: | 1271 |
| Página de fin: | 1275 |
| DOI: | http://dx.doi.org/10.1242/jeb.052357 |
| Título revista: | Journal of Experimental Biology |
| Título revista abreviado: | J. Exp. Biol. |
| ISSN: | 00220949 |
| CODEN: | JEBIA |
| CAS: | oxygen, 7782-44-7; Oxygen, 7782-44-7 |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v214_n8_p1271_Schilman.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v214_n8_p1271_Schilman |
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Citas:
---------- APA ----------
Schilman, P.E., Waters, J.S., Harrison, J.F. & Lighton, J.R.B. (2011) . Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster. Journal of Experimental Biology, 214(8), 1271-1275.http://dx.doi.org/10.1242/jeb.052357
---------- CHICAGO ----------
Schilman, P.E., Waters, J.S., Harrison, J.F., Lighton, J.R.B. "Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster" . Journal of Experimental Biology 214, no. 8 (2011) : 1271-1275.http://dx.doi.org/10.1242/jeb.052357
---------- MLA ----------
Schilman, P.E., Waters, J.S., Harrison, J.F., Lighton, J.R.B. "Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster" . Journal of Experimental Biology, vol. 214, no. 8, 2011, pp. 1271-1275.http://dx.doi.org/10.1242/jeb.052357
---------- VANCOUVER ----------
Schilman, P.E., Waters, J.S., Harrison, J.F., Lighton, J.R.B. Effects of temperature on responses to anoxia and oxygen reperfusion in Drosophila melanogaster. J. Exp. Biol. 2011;214(8):1271-1275.http://dx.doi.org/10.1242/jeb.052357
