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Abstract:

The effects of dopamine (DA) and dopaminergic agonists and antagonists on ion transport were studied in isolated perfused gills of the crab Chasmagnathus granulatus. DA applied under steady state conditions (perfusion with hemolymph-like saline) produced a transient increase of the transepithelial potential difference (Vtc) from 2.2±0.2 to 4.8±0.3 mV, describing an initial cAMP-dependent stimulating phase followed by an inhibitory phase. Spiperone and domperidone (antagonists of D2-like DA receptors in vertebrates) completely blocked the response to DA, while the D1-like antagonist SCH23390 blocked only the inhibitory phase. Theophylline (phosphodiesterase inhibitor) and okadaic acid (protein phosphatases PP1 and PP2A inhibitor) were also able to block the inhibitory phase, suggesting that it depends on adenylyl cyclase inhibition and on protein phosphatases. When the gills were perfused with hypoosmotic solution, or with the adenylyl cyclase activator forskolin, Vte was increased several-fold. DA applied under these stimulated conditions partially reversed the Vte increase by 54% and 25%, respectively. Similarly, the D1-like agonist, fenoldopam, produced a 33% reduction in the stimulated Vte. We propose that, in C. granulatus gills, DA stimulates adenylyl cyclase and therefore ion transport through D1-like receptors linked to a Gs protein, although they respond to antagonists that interact with D2-like receptors in vertebrates. The inhibitory phase seems to be mediated by D2-like receptors linked to a Gi/o protein, which inhibits adenylyl cyclase, although these receptors can be activated or blocked by agonists or antagonists that interact with D1-like receptors in vertebrates and insects.

Registro:

Documento: Artículo
Título:Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors
Autor:Genovese, G.; Senek, M.; Ortiz, N.; Regueira, M.; Towle, D.W.; Tresguerres, M.; Luquet, C.M.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, (C1428EHA) Buenos Aires, Argentina
CONICET (Consejo Nacional de Investigaciones Científicas Y Técnicas), (C1033AAJ) Buenos Aires, Argentina
Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, United States
Centro Nacional Patagónico (CONICET), Chubut Province 9120, Argentina
Department of Biological Sciences, University of Alberta, Canada
AUSMA, University of Comahue, Neuquén Province 8370, Argentina
Palabras clave:cAMP; Chasmagnathus granulatus; Crab; Dopamine antagonist; Dopamine receptor dopamine agonist; Na +/K+-ATPase; Okadaic acid; Transepithelial potential differences; adenosine triphosphatase (potassium sodium); cyclic AMP dependent protein kinase; dopamine 1 receptor; dopamine 2 receptor; okadaic acid; theophylline; animal; article; Brachyura; gill; ion transport; male; metabolism; osmolarity; physiology; signal transduction; Animals; Brachyura; Cyclic AMP-Dependent Protein Kinases; Gills; Ion Transport; Male; Na(+)-K(+)-Exchanging ATPase; Okadaic Acid; Osmolar Concentration; Receptors, Dopamine D1; Receptors, Dopamine D2; Signal Transduction; Theophylline; Chasmagnathus granulata; Decapoda (Crustacea); Hexapoda; Insecta; Vertebrata
Año:2006
Volumen:209
Número:14
Página de inicio:2785
Página de fin:2793
DOI: http://dx.doi.org/10.1242/jeb.02308
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:okadaic acid, 78111-17-8; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Cyclic AMP-Dependent Protein Kinases, EC 2.7.1.37; Na(+)-K(+)-Exchanging ATPase, EC 3.6.1.37; Okadaic Acid, 78111-17-8; Receptors, Dopamine D1; Receptors, Dopamine D2; Theophylline, 58-55-9
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v209_n14_p2785_Genovese.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v209_n14_p2785_Genovese

Referencias:

  • Asghar, M., Hussain, T., Lokhandwala, M.F., Activation of dopamine D1-like receptor causes phosphorylation of α1-subunit of Na+, K+-ATPase in rat renal proximal tubules (2001) Eur. J. Pharmacol., 411, pp. 61-66
  • Bertorello, A.M., Katz, A.I., Regulation of Na+-K+ pump activity: Pathways between receptors and effectors (1995) News Physiol. Sci., 10, pp. 253-260
  • Bianchini, A., Gilles, R., Cyclic AMP as a modulator of NaCl transport in gills of the euryhaline Chinese crab Eriocheir sinensis (1990) Mar. Biol., 104, pp. 191-195
  • Boschi, E.E., Los crustaceos decápodos brachyura del litoral bonaerense (1964) Bol. Inst. Biol. Mar., 164, pp. 1-34
  • Charmantier, G., Gimenez, L., Charmantier-Daures, M., Anger, K., Ontogeny of osmoregulation, physiological plasticity and larval export strategy in the grapsid crab Chasmagnathus granulata (Crustacea, Decapoda) (2002) Mar. Ecol. Prog. Ser., 229, pp. 185-194
  • Cohen, P., Holmes, C.F.B., Tsukitani, Y., Okadaic acid: A new probe for the study of cellular regulation (1990) Trends Biochem. Sci., 15, pp. 98-102
  • Drach, P., Tchernigovtzeff, C., Sur la méthode de détermination des stades d'intermue et son application générale aux crustacés (1967) Vie Milieu, 18, pp. 597-607
  • Eckhardt, E., Pierrot, C., Thuet, P., Van Herp, F., Charmantier-Daures, M., Trilles, J.P., Charmantier, G., Stimulation of osmoregulating processes in the perfused gill of the crab Pachygrapsus marmoratus (Crustacea, Decapoda) by a sinus gland peptide (1995) Gen. Comp. Endocrinol., 99, pp. 169-177
  • Ewart, H.S., Klip, A., Hormonal regulation of the Na+-K+-ATPase: Mechanisms underlying rapid and sustained changes in pump activity (1995) Am. J. Physiol. Cell Physiol., 269, pp. 295-311
  • Fingerman, M., Crustacean endocrinology: A retrospective, prospective, and introspective analysis (1997) Physiol. Zool., 70, pp. 257-269
  • Halperin, J., Genovese, G., Tresguerres, M., Luquet, C.M., Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP (2004) Comp. Biochem. Physiol., 139 A, pp. 103-109
  • Hussain, T., Lokhandwala, M.F., Renal dopamine DA1 receptor coupling with G(S) and G(q/11) proteins in spontaneously hypertensive rats (1997) Am. J. Physiol. Renal Physiol., 272, pp. 339-346
  • Hussain, T., Abdul-Wahab, R., Lokhandwala, M.F., Bromocriptine stimulates Na+, K+-ATPase in renal proximal tubules via the cAMP pathway (1997) Eur. J. Pharmacol., 321, pp. 259-263
  • Iorio, L.C., Barnett, A., Leitz, F.H., Houser, V.P., Korduba, C.A., SCH 23390, a potential benzazepine antipsychotic with unique interactions on dopaminergic systems (1983) J. Pharmacol. Exp. Ther., 226, pp. 462-468
  • Kamemoto, F.I., Oyama, S.N., Neuroendocrine influence on effector tissues of hydromineral balance in crustaceans (1985) Current Trends in Comparative Endocrinology, pp. 883-886. , ed. B. Lofts and W. N. Holmes, Hong Kong: Hong Kong University Press
  • Kuo, C.M., Hsu, C.R., Lin, C.Y., Hyperglycaemic effects of DA in tiger shrimp, Penaeus monodon (1995) Aquaculture, 135, pp. 161-172
  • Laurenza, A., Sutkowski, E.M., Seamon, K.B., Forskolin: A specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action? (1989) Trends Pharmacol. Sci., 10, pp. 442-447
  • Lohrmann, D.M., Kamemoto, F.I., The effect of dibutyryl cAMP on sodium uptake by isolated perfused gills of Callinictes sapidus (1987) Gen. Comp. Endocrinol., 65, pp. 300-305
  • Luquet, C.M., Ansaldo, M., Acid-base balance and ionic regulation during emersion in the estuarine intertidal crab Chasmagnathus granulata Dana (Decapoda Grapsidae) (1997) Comp. Biochem. Physiol., 117 A, pp. 407-410
  • Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R., Siebers, D., Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity (2002) J. Exp. Biol., 205, pp. 71-77
  • Mantel, L.H., Neurohormonal integration of osmotic and ionic regulation (1985) Am. Zool., 25, pp. 253-263
  • Missale, C., Nash, S.R., Robinson, S.W., Jaber, J., Caron, M.G., Dopamine receptors: From structure to function (1998) Physiol. Rev., 78, pp. 189-225
  • Mo, J.L., Devos, P., Trausch, G., Dopamine as a modulator of ionic transport and Na+/K +-ATPase activity in the gills of the Chinese crab Eriocheir sinensis (1998) J. Crust. Biol., 18, pp. 442-448
  • Mo, J.L., Devos, P., Trausch, G., Dopamine D1 receptors in the gills of Chinese crab Eriocheir sinensis (2002) Comp. Biochem. Physiol., 131 C, pp. 433-438
  • Morris, S., Neuroendocrine regulation of osmoregulation and the evolution of air-breathing in decapod crustaceans (2001) J. Exp. Biol., 204, pp. 979-989
  • Morris, S., Edwards, T., Control of osmoregulation via regulation of Na+/K +-ATPase activity in the amphibious purple shore crab Leptograpsus variegatus (1995) Comp. Biochem. Physiol., 112 C, pp. 129-136
  • Mustard, J.A., Beggs, K.T., Mercer, A.R., Molecular biology of the invertebrate dopamine receptors (2005) Arch. Insect Biochem. Physiol., 59, pp. 103-117
  • Onken, H., Schobel, A., Kraft, J., Putzenlechner, M., Active NaCl absorption across split lamellae of posterior gills of the Chinese crab Eriocheir sinensis: Stimulation by eyestalk extract (2000) J. Exp. Biol., 203, pp. 1373-1381
  • Onken, H., Tresguerres, M., Luquet, C.M., Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus (2003) J. Exp. Biol., 206, pp. 1017-1023
  • Riestenpatt, S., Zeiske, W., Onken, H., Cyclic AMP stimulation of electrogenic uptake of Na+ and Cl- across the gill epithelium of the Chinese crab Eriocheir sinensis (1994) J. Exp. Biol., 188, pp. 159-174
  • Rodriguez, E.M., Medesani, D.A., López Greco, L.S., Fingerman, M., Effects of some steroids and other compounds on ovarian growth of the red swamp crayfish, Procambarus clarkii, during early vitellogenesis (2002) J. Exp. Zool., 292, pp. 82-87
  • Savage, J.P., Robinson, G.D., Inducement of increased gill Na+/K+-ATPase activity by a hemolymph factor in hyperosmoregulating Callinectes sapidus (1983) Comp. Biochem. Physiol., 75, pp. 65-69
  • Siebers, D., Winkler, A., Lucu, C., Thedens, G., Weichart, D., Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas (1985) Mar. Biol., 87, pp. 185-192
  • Sommer, M.J., Mantel, L.H., Effect of dopamine, cyclic AMP, and pericardial organs on sodium uptake and Na/K-ATPase activity in gills of the green crab Carcinus maenas (L) (1988) J. Exp. Zool., 248, pp. 272-277
  • Sommer, M.J., Mantel, L.H., Effects of dopamine and acclimation to reduced salinity on the concentration of cyclic AMP in the gills of the green crab, Carcinus maenas (L) (1991) Gen. Comp. Endocrinol., 82, pp. 364-368
  • Spanings-Pierrot, C., Van Herp, F., Gompel, M., Skaret, G., Charmantier, G., Involvement of the crustacean hyperglycemic hormone in the control of gill ion transport in the crab Pachygrapsus marmoratus (2000) Gen. Comp. Endocrinol., 119, pp. 340-350
  • Therien, A.G., Blostein, R., Mechanisms of sodium pump regulation (2000) Am. J. Physiol. Cell Physiol., 279, pp. 541-566
  • Towle, D.W., Paulsen, R.S., Weihrauch, D., Kordylewski, M., Salvador, C., Lignot, J.H., Spanings-Pierrot, C., Na++K+-ATPase in gills of the blue crab Callinectes sapidus: CDNA sequencing and salinity-related expression of α-subunit mRNA and protein (2001) J. Exp. Biol., 204, pp. 4005-4012
  • Trausch, G., Forget, M.-C., Devos, P., Bioamines-stimulated phosphorylation and (Na+, K +)-ATPase in the gills of the Chinese crab, Eriocheir sinensis (1989) Comp. Biochem. Physiol., 94, pp. 487-492
  • Tresguerres, M., Onken, H., Pérez, A.F., Luquet, C.M., Electrophysiology of posterior, Na-Cl absorbing gills Chasmagnathus granulatus: Rapid responses to osmotic variations (2003) J. Exp. Biol., 206, pp. 619-626
  • Wilkens, J.L., Kuramoto, T., McMahon, B.R., The effects of six pericardial hormones and hypoxia on the semi-isolated heart and sternal arterial valve of the lobster Homarus americanus (1996) Comp. Biochem. Physiol., 114, pp. 57-65
  • Zatta, P., Dopamine, noradrenaline and serotonin during hypo-osmotic stress of Carcinus maenas (1987) Mar. Biol., 96, pp. 479-481

Citas:

---------- APA ----------
Genovese, G., Senek, M., Ortiz, N., Regueira, M., Towle, D.W., Tresguerres, M. & Luquet, C.M. (2006) . Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors. Journal of Experimental Biology, 209(14), 2785-2793.
http://dx.doi.org/10.1242/jeb.02308
---------- CHICAGO ----------
Genovese, G., Senek, M., Ortiz, N., Regueira, M., Towle, D.W., Tresguerres, M., et al. "Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors" . Journal of Experimental Biology 209, no. 14 (2006) : 2785-2793.
http://dx.doi.org/10.1242/jeb.02308
---------- MLA ----------
Genovese, G., Senek, M., Ortiz, N., Regueira, M., Towle, D.W., Tresguerres, M., et al. "Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors" . Journal of Experimental Biology, vol. 209, no. 14, 2006, pp. 2785-2793.
http://dx.doi.org/10.1242/jeb.02308
---------- VANCOUVER ----------
Genovese, G., Senek, M., Ortiz, N., Regueira, M., Towle, D.W., Tresguerres, M., et al. Dopaminergic regulation of ion transport in gills of the euryhaline semiterrestrial crab Chasmagnathus granulatus: Interaction between D1- and D2-like receptors. J. Exp. Biol. 2006;209(14):2785-2793.
http://dx.doi.org/10.1242/jeb.02308