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Abstract:

Using quantitative real-time PCR, the expression of mRNAs encoding three transport-related proteins and one putative housekeeping protein was analyzed in anterior and posterior gills of the euryhaline crab Chasmagnathus granulatus following transfer from isosmotic conditions (30‰ salinity) to either dilute (2‰) or concentrated (45‰) seawater. Modest changes were observed in the abundance of mRNAs encoding the housekeeping protein arginine kinase and the vacuolar-type H+-ATPase B-subunit, both of which were highly expressed under all conditions. By contrast, the expression of Na +/K+-ATPase α-subunit mRNA and Na+/K +/2Cl- cotransporter mRNA was strongly responsive to external salinity. During acclimation to dilute seawater, cotransporter mRNA increased 10-20-fold in posterior gills within the first 24 h while Na +/K+-ATPase α-subunit mRNA increased 35-55-fold. During acclimation to concentrated seawater, cotransporter mRNA increased 60-fold by 96 h and Na+/K+-ATPase α-subunit increased approximately 25-fold in posterior gills. Our results indicate a complex pattern of transcriptional regulation dependent upon the direction of salinity change and the developmental background of the gills.

Registro:

Documento: Artículo
Título:Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus
Autor:Luquet, C.M.; Weihrauch, D.; Senek, M.; Towle, D.W.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
CONICET (Consejo Nacional de Investigaciones Cientificas Y Tecnicas), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
Department of Animal Physiology, University of Osnabrueck, 49076 Osnabrueck, Germany
College of the Atlantic, Bar Harbor, ME 04609, United States
Mount Desert Island Biological Laboratory, Salsbury Cove, ME 04672, United States
AUSMA-COMAHUE, Pasaje de la Paz 235, 8370 Neuquén, Argentina
Palabras clave:Arginine kinase; Crab; Gene expression; Gill; Na+/K+-ATPase; Na+/K+/2Cl - cotransporter; Quantitative PCR; V-type H+-ATPase; messenger RNA; primer DNA; sea water; acclimatization; analysis of variance; animal; article; Brachyura; chemistry; comparative study; gene expression regulation; genetics; gill; ion transport; metabolism; polymerase chain reaction; time; Acclimatization; Analysis of Variance; Animals; Brachyura; DNA Primers; Gene Expression Regulation; Gills; Ion Pumps; Polymerase Chain Reaction; RNA, Messenger; Seawater; Time Factors; Chasmagnathus granulata; Decapoda (Crustacea)
Año:2005
Volumen:208
Número:19
Página de inicio:3627
Página de fin:3636
DOI: http://dx.doi.org/10.1242/jeb.01820
Handle:http://hdl.handle.net/20.500.12110/paper_00220949_v208_n19_p3627_Luquet
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:DNA Primers; Ion Pumps; RNA, Messenger
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v208_n19_p3627_Luquet.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v208_n19_p3627_Luquet

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Citas:

---------- APA ----------
Luquet, C.M., Weihrauch, D., Senek, M. & Towle, D.W. (2005) . Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus. Journal of Experimental Biology, 208(19), 3627-3636.
http://dx.doi.org/10.1242/jeb.01820
---------- CHICAGO ----------
Luquet, C.M., Weihrauch, D., Senek, M., Towle, D.W. "Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus" . Journal of Experimental Biology 208, no. 19 (2005) : 3627-3636.
http://dx.doi.org/10.1242/jeb.01820
---------- MLA ----------
Luquet, C.M., Weihrauch, D., Senek, M., Towle, D.W. "Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus" . Journal of Experimental Biology, vol. 208, no. 19, 2005, pp. 3627-3636.
http://dx.doi.org/10.1242/jeb.01820
---------- VANCOUVER ----------
Luquet, C.M., Weihrauch, D., Senek, M., Towle, D.W. Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus. J. Exp. Biol. 2005;208(19):3627-3636.
http://dx.doi.org/10.1242/jeb.01820