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Abstract:

Split lamellae of posterior gills of Chasmagnathus granulatus adapted to 2.5‰ salinity were mounted in a modified Ussing chamber. With NaCl-saline on both sides of the preparation a transepithelial voltage (Vte) of 4.1±0.5 mV (outside positive) was measured. After voltage-clamping, the negative short-circuit current (Isc) amounted to -142±21 μA cm-2 at a conductance (Gte) of 44±5 mS cm-2. Substitution of either chloride (by nitrate) or sodium (by choline) on both sides of split gill lamellae significantly reduced Isc (by 70-80%) and Gte (by 30-50%). External CsCl (but not BaCl2 or furosemide) inhibited the negative Isc without affecting Gte. Addition of ouabain, BaCl2 or diphenylamine-2-carboxylate to the internal bath inhibited Isc at unchanged Gte. Internal acetazolamide did not affect Isc or Gte across split gill lamellae. Unidirectional Na+ influx across isolated and perfused posterior gills, however, was reduced by internal acetazolamide by approximately 20% at constant Vte. The results suggest that posterior gills of hyperosmoregulating C. granulatus display a high conductance epithelium that actively absorbs NaCl in a coupled way by an electrogenic mechanism similar to that seen in the thick ascending limb of Henle's loop and, to a minor degree, by an electroneutral mechanism, presumably via apical Na+/H+and Cl-/HCO3--antiports.

Registro:

Documento: Artículo
Título:Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus
Autor:Onken, H.; Tresguerres, M.; Luquet, C.M.
Filiación:Departamento de Biologia, Faculdade de Filosofia, Universidade de São Paulo, Avenida Bandeirantes 3900, Ribeirão Preto 14040-901, SP, Brazil
Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina
Palabras clave:Chasmagnathus granulatus; Crab gill; Crustacea; Flux measurement; Inhibitor; Ion substitution; Ion transport; Osmoregulation; Short-circuit current; Transepithelial conductance; Ussing chamber; acetazolamide; anthranilic acid derivative; fenamic acid; ouabain; sodium chloride; absorption; active transport; animal; article; Brachyura; comparative study; electrolyte balance; electrophysiology; gill; ion transport; metabolism; physiology; Absorption; Acetazolamide; Animals; Anthranilic Acids; Biological Transport, Active; Brachyura; Electrophysiology; Gills; Ion Transport; Ouabain; Sodium Chloride; Water-Electrolyte Balance; Animalia; Brachyura; Chasmagnathus granulata; Crustacea; Decapoda (Crustacea); Strophanthus gratus
Año:2003
Volumen:206
Número:6
Página de inicio:1017
Página de fin:1023
DOI: http://dx.doi.org/10.1242/jeb.00227
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:acetazolamide, 1424-27-7, 59-66-5; ouabain, 11018-89-6, 630-60-4; sodium chloride, 7647-14-5; Acetazolamide, 59-66-5; Anthranilic Acids; fenamic acid, 91-40-7; Ouabain, 630-60-4; Sodium Chloride, 7647-14-5
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v206_n6_p1017_Onken.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v206_n6_p1017_Onken

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Citas:

---------- APA ----------
Onken, H., Tresguerres, M. & Luquet, C.M. (2003) . Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus. Journal of Experimental Biology, 206(6), 1017-1023.
http://dx.doi.org/10.1242/jeb.00227
---------- CHICAGO ----------
Onken, H., Tresguerres, M., Luquet, C.M. "Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus" . Journal of Experimental Biology 206, no. 6 (2003) : 1017-1023.
http://dx.doi.org/10.1242/jeb.00227
---------- MLA ----------
Onken, H., Tresguerres, M., Luquet, C.M. "Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus" . Journal of Experimental Biology, vol. 206, no. 6, 2003, pp. 1017-1023.
http://dx.doi.org/10.1242/jeb.00227
---------- VANCOUVER ----------
Onken, H., Tresguerres, M., Luquet, C.M. Active NaCl absorption across posterior gills of hyperosmoregulating Chasmagnathus granulatus. J. Exp. Biol. 2003;206(6):1017-1023.
http://dx.doi.org/10.1242/jeb.00227