Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In the present study, the influence of short-term osmotic variations on some electrophysiological properties related to NaCl absorption across posterior gills of Chasmagnathus granulatus was investigated. The transepithelial potential difference (V te ) of isolated and perfused gills increased significantly when hyposmotic saline (699 mosmol l -1 ) was used instead of isosmotic Solution (1045 mosmol l -1 ). A reduction of the concentration of Na + or Cl - at constant osmolarity did not produce any change in V te . Transepithelial short-circuit current (I sc ) and conductance (G te ), measured with split gill lamellae mounted in a modified Ussing chamber, also increased after changing to hyposmotic salines (I sc : from -89.0±40.8 μAcm -2 to -179.3±37.0μAcm -2 ; G te : from 40.5±16.9 mS cm -2 to 47.3±15.8 mS cm -2 ). The observed effects of reduced osmolarity were fast, reversible and gradually dependent on the magnitude of the osmotic variation. The acitivity of the Na + /K + -ATPase increased significantly after perfusion with hyposmotic saline, from 18.73±6.35 μmol P i h -1 mg -1 to 41.84±14.54 μmol P i h -1 mg -1 . Theophylline maintained part of the elevated V te induced by hyposmotic saline, suggesting that an increased cellular cyclic AMP level is involved in the response to reduced osmolarity. In summary, the results indicate that the hemolymph osmolarity regulates active transbranchial NaCl absorption by modulating the activity of the basolateral Na + /K + -ATPase and by changing a conductive pathway, probably at the apical membrane.

Registro:

Documento: Artículo
Título:Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
Autor:Tresguerres, M.; Onken, H.; Pérez, A.F.; Luquet, C.M.
Filiación:Dept. Biodiv./Experimental Biology, F.C.E.N. University of Buenos Aires, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina
Palabras clave:Chasmagnathus granulatus; Crab; Cyclic AMP; Gills; Hyperosmoregulation; Na + /K + -ATPase; Perfused gills; Short-circuit current; Split gill lamellae; Transepithelial conductance; Transepithelial voltage; adenosine triphosphatase (potassium sodium); cyclic AMP; sodium chloride; animal; article; Brachyura; cell membrane potential; drug effect; electrophysiology; gill; male; metabolism; methodology; osmotic pressure; physiology; Animals; Brachyura; Cyclic AMP; Electrophysiology; Gills; Male; Membrane Potentials; Na(+)-K(+)-Exchanging ATPase; Osmotic Pressure; Sodium Chloride; Animalia; Brachyura; Chasmagnathus granulata; Decapoda (Crustacea)
Año:2003
Volumen:206
Número:3
Página de inicio:619
Página de fin:626
DOI: http://dx.doi.org/10.1242/jeb.00125
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:cyclic AMP, 60-92-4; sodium chloride, 7647-14-5; Cyclic AMP, 60-92-4; Na(+)-K(+)-Exchanging ATPase, EC 3.6.1.37; Sodium Chloride, 7647-14-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v206_n3_p619_Tresguerres

Referencias:

  • Baldwin, J.F., Kirschner, L.B., Sodium and chloride regulation in Uca adapted to 175% sea water (1976) J. Exp. Zool., 49, pp. 158-171
  • Baldwin, J.F., Kirschner, L.B., Sodium and chloride regulation in Uca adapted to 10% sea water (1976) J. Exp. Zool., 49, pp. 172-180
  • Bonting, S.L., Cavaggio, L.L., Studies on the Na,K-activated ATPase. Correlation with cation fluxes in tissues (1963) Arch. Biochem. Biophys., 101, pp. 293-305
  • Carranza, M.L., Rousselot, M., Chihalin, A.V., Bertorello, A.M., Favre, H., Feraille, E., Protein kinase A induces recruitment of active Na+,K+-ATPase units to the plasma membrane of rat proximal convoluted tubule cells (1998) J. Physiol., 511, pp. 235-243
  • Charmantier, G., Gimenez, L., Charmantier-Daures, M., Anger, K., Ontogeny of osmoregulation, physiological plasticity and larval export strategy in the grapsid crab Chasmagnathus granulata (Crustacea, Decapoda) (2002) Mar. Ecol. Prog. Ser., 229, pp. 185-194
  • Cheng, S.X.J., Aizman, O., Nairn, A.C., Greengard, P., Aperia, A., [Ca 2+ ] i determines the effects of protein kinase A and C on activity of rat renal Na + . K + -ATPase (1999) J. Physiol., 518, pp. 37-46
  • Coutry, N., Farman, N., Bonvalet, J.P., Blot-Chahaund, M., Role of cell volume variations in Na(+)-K(+)-ATPase recruitment and/or activation in cortical collecting duct (1994) Am. J. Physiol., 266 C, pp. 1342-1349
  • De Wolf, I., Van Driessche, W., Voltage-dependent Ba 2+ block of K + channels in apical membrane of frog skin (1986) Am. J. Physiol., 251 C, pp. 696-706
  • Drach, P., Tchernigovtzeff, C., Sur la mèthode de détermination des stades d'intermue et son application générale aux crustacés (1967) Vie Milieu, 18, pp. 597-607
  • Evans, D.H., Cooper, K., Bogan, M.B., Sodium extrusion by the sea-water-acclimated fiddler crab Uca pugilator: Comparison with other marine crustacea and marine teleost fish (1976) J. Exp. Biol., 64, pp. 203-219
  • Green, J.W., Harsch, H., Barr, L., Prosser, C.L., The regulation of water and salt by the fiddler crabs Uca pugnax and Uca pugilator (1959) Biol. Bull., 116, pp. 76-87
  • Greger, R., Ion transport mechanisms in thick ascending limb of Henle's loop of mammalian nephron (1985) Physiol. Rev., 65, pp. 760-797
  • Halperin, J., Ansaldo, M., Pellerano, G.N., Luquet, C.M., Bimodal breathing in the estuarine crab Chasmagnathus granulatus Dana 1851 - Physiological and morphological studies (2000) Comp. Biochem. Physiol. A, 126, pp. 341-349
  • Johnsen, A., Nielsen, R., Effects of antidiuretic hormone, arginine vasotocin, theophylline, fillipin and A 23187 on cyclic AMP in isolated frog skin epithelium (R. temporaria) (1978) Acta Physiol. Scand., 102, pp. 281-289
  • Kamemoto, F.I., Neuroendocrinology of osmoregulaton in crabs (1991) Zool. Sci., 8, pp. 827-833
  • Lang, F., Busch, G.L., Ritter, M., Völkl, H., Waldegger, S., Gulbins, E., Häussinger, D., Functional significance of cell volume regulatory mechanisms (1998) Physiol. Rev., 78, pp. 247-306
  • Lowry, O.H., Rosenhrough, N.J., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Lueu, C., Flik, G., Na + -K + -ATPase and Na + /Ca + exchange activities in gills of hyperegulating Carcinus maenas (1999) Am. J. Physiol., 276 R, pp. 490-499
  • Luquet, C.M., Ansaldo, M., Acid-base balance and ionic regulation during emersion in the estuarine intertidal crab Chasmagnathus granulata Dana (Decapoda Grapsidae) (1997) Comp. Biochem. Physiol. A, 117, pp. 407-410
  • Luquet, C.M., Postel, U., Halperín, J., Urcola, M.R., Marques, R., Siebers, D., Transepithelial potential differences and sodium flux in isolated peffused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-saline media (2002) J. Exp. Biol., 205, pp. 71-77
  • Macknight, A.D.C., Volume regulation in epithelia (1991) Advances in Comparative and Environmental Physiology, 9, pp. 3-42. , ed. R. Gilles, E. K. Hoffmann and L. Bolls. Berlin, Heidelberg: Springer
  • Mo, J.L., Devos, P., Trausch, G., Dopamine as a modulator of ionic transport and Na + /K + - ATPase activity in the gills of the chinese crab Eriocheir sinensis (1998) J. Crust. Biol., 18, pp. 442-448
  • Morris, S., Neuroendocrine regulation of osmoregulation and the evolution of air-breathing in decapod crustaceans (2001) J. Exp. Biol., 204, pp. 979-989
  • Mougabure Cueto, G.A., (1998) Interacción Entre la Regulación Iónica y el Equilibrio Ácido-base en el Cangrejo Chasmagnathus Granulata Dana, 1851 (Decapoda, Grapsidae), , Graduate Thesis. Universidad de Buenos Aires
  • Onken, H., Active and electrogenic absorption of Na + and Cl - across posterior gills of Eriocheir sinensis: Influence of short-term osmotic variations (1996) J. Exp. Biol., 199, pp. 901-910
  • Onken, H., Graszynski, K., Johannsen, M., Putzenlechner, M., Riestenpatt, S., Schirmer, C., Siebers, D., Zeiske, W., How to overcome osmotic stress? Marine crabs conquer freshwater. New insights from modern electrophysiology (1995) Helgoländer Meeresunters, 49, pp. 715-725
  • Onken, H., Graszynski, K., Zeiske, W., Na + -independent electrogenic Cl - uptake across the posterior gills of the Chinese crab (Eriocheir sinensis): Voltage-clamp and microelectrode studies (1991) J. Comp. Physiol. B, 161, pp. 293-301
  • Onken, H., Riestenpatt, S., NaCl absorption across split gill lamellae of hyperregulating crabs: Transport mechanisms and their regulation (1998) Comp. Biochem. Physiol. A, 119, pp. 883-893
  • Onken, H., Schobel, A., Kraft, J., Putzenlechner, M., Active NaCl absorption across split lamellae of posterior gills of the Chinese crab Eriocheir sinensis: Stimulation by eyestalk extract (2000) J. Exp. Biol., 203, pp. 1373-1381
  • Péqueux, A., Osmotic regulation in crustaceans (1995) J. Crust. Biol., 15, pp. 1-60
  • Riestenpatt, S., Onken, H., Siebers, D., Active absorption of Na + and Cl - across the gill epithelium of the shore crab Carcinus maenas: Voltage-clamp and ion-flux studies (1996) J. Exp. Biol., 199, pp. 1545-1554
  • Rodriguez Moreno, P.A., Scwarzhaum, P.J., Rodriguez, E.M., Effects of cadmium on gill Na, K-ATPase of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae) during postmolt: In vivo and in vitro studies (1998) Bull. Environ. Contam. Toxicol., 61, pp. 629-636
  • Schmidt, A.S.C., Santos, E.A., Behaviour and haemolymphatic ionic composition of the intertidal crab Chasmagnathus granulata Dana, 1851 (Crustacea: Decapoda) during emersion (1993) Comp. Biochem. Physiol. A, 106, pp. 337-342
  • Schwarz, H.J., Graszynski, K., Ion transport in crab gills: A new method using isolated half platelets of Eriocheir gills in an Ussing-type chamber (1989) Comp. Biochem. Physiol. A, 92, pp. 601-604
  • Seamon, K.B., Padgett, W., Daly, J.W., Forskolin: Unique diterpene activator of adenylate cyclase in membranes and in intact cells (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 3363-3367
  • Siebers, D., Winkler, A., Lucu, C., Thedens, G., Welchart, D., Na-K-ATPase generates an active transport potential in the gills of the hyperregulating shore crab Carcinus maenas (1985) Mar. Biol., 87, pp. 185-192
  • Sommer, M.J., Mantel, L.H., Effect of dopamine, cyclic AMP, and pericarcial organs on sodium uptake and Na/K-ATPase activity in gills of the green crab Carcinus maenas (L) (1988) J. Exp. Zool., 248, pp. 272-277
  • Trausch, G., Forget, M.C., Devos, P., Bioamine-stimulated phosphorylation and (Na + , K + )-ATPase in the gills of the Chinese crab, Eriocheir sinensis (1989) Comp. Biochem. Physiol. B, 94, pp. 487-492
  • Ussing, H.H., Relationship between osmotic reactions and active sodium transport in the frog skin epithelium (1965) Acta Physiol. Scand., 63, pp. 141-155
  • Venosa, R.A., Hypo-osmotic stimulation of active Na+ transport in frog muscle: Apparent upregulation of Na+ pumps (1991) J. Membr. Biol., 120, pp. 97-104
  • Watson, P.A., Accumulation of cAMP and calcium in S49 mouse lymphoma cells following hyposmotic swelling (1989) J. Biol. Chem., 264, pp. 14735-14740
  • Watson, P.A., Direct stimulation of adenylate cyclase by mechanical forces in S49 mouse lymphoma cells during hyposmotic swelling (1990) J. Biol. Chem., 265, pp. 6569-6575
  • Whalley, D.W., Hool, L.C., Ten Eick, R.E., Rasmussen, H.H., Effect of osmotic swelling and shrinkage on Na(+)-K+ pump activity in mammalian cardiac myocytes (1993) Am. J. Physiol., 265 C, pp. 1201-1210

Citas:

---------- APA ----------
Tresguerres, M., Onken, H., Pérez, A.F. & Luquet, C.M. (2003) . Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations. Journal of Experimental Biology, 206(3), 619-626.
http://dx.doi.org/10.1242/jeb.00125
---------- CHICAGO ----------
Tresguerres, M., Onken, H., Pérez, A.F., Luquet, C.M. "Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations" . Journal of Experimental Biology 206, no. 3 (2003) : 619-626.
http://dx.doi.org/10.1242/jeb.00125
---------- MLA ----------
Tresguerres, M., Onken, H., Pérez, A.F., Luquet, C.M. "Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations" . Journal of Experimental Biology, vol. 206, no. 3, 2003, pp. 619-626.
http://dx.doi.org/10.1242/jeb.00125
---------- VANCOUVER ----------
Tresguerres, M., Onken, H., Pérez, A.F., Luquet, C.M. Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations. J. Exp. Biol. 2003;206(3):619-626.
http://dx.doi.org/10.1242/jeb.00125