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Abstract:

We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6-8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10%‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, -10.2±1.6, -10.8±1.3 and -6.7±1.3mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30%‰ to 20%‰ or 10%‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2 (5mmoll-1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 μequiv g-1h-1. Gill 6 of crabs acclimated to high salinity produced TEPDs of -1.5±0.1 and -1.3±0.09mV after perfusion with 30%‰ or 40%‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 μequiv g-1h-1 was recorded in gill 6 perfused with 38%‰ saline.

Registro:

Documento: Artículo
Título:Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity
Autor:Luquet, C.M.; Postel, U.; Halperin, J.; Urcola, M.R.; Marques, R.; Siebers, D.
Filiación:Department of Biological Sciences, FCEN University of Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina
Palabras clave:Chasmagnathus granulatus; Crab; Hyper-regulation; Hypo-regulation; Ion flux; Isolated perfused gill; Na+K+-ATPase; Ouabain; Transepithelial potential difference; adenosine triphosphatase (potassium sodium); hypotonic solution; sodium; sodium chloride; adaptation; animal; Argentina; article; Brachyura; cell membrane potential; epithelium; gill; metabolism; physiology; Adaptation, Physiological; Animals; Argentina; Brachyura; Epithelium; Gills; Hypotonic Solutions; Membrane Potentials; Na(+)-K(+)-Exchanging ATPase; Saline Solution, Hypertonic; Sodium; Sodium Chloride; Animalia; Brachyura; Chasmagnathus granulata; Decapoda (Crustacea); Grapsidae; Strophanthus gratus
Año:2002
Volumen:205
Número:1
Página de inicio:71
Página de fin:77
Título revista:Journal of Experimental Biology
Título revista abreviado:J. Exp. Biol.
ISSN:00220949
CODEN:JEBIA
CAS:Hypotonic Solutions; Na(+)-K(+)-Exchanging ATPase, EC 3.6.1.37; Saline Solution, Hypertonic; Sodium Chloride, 7647-14-5; Sodium, 7440-23-5
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00220949_v205_n1_p71_Luquet.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet

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Citas:

---------- APA ----------
Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R. & Siebers, D. (2002) . Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity. Journal of Experimental Biology, 205(1), 71-77.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet [ ]
---------- CHICAGO ----------
Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R., Siebers, D. "Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity" . Journal of Experimental Biology 205, no. 1 (2002) : 71-77.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet [ ]
---------- MLA ----------
Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R., Siebers, D. "Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity" . Journal of Experimental Biology, vol. 205, no. 1, 2002, pp. 71-77.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet [ ]
---------- VANCOUVER ----------
Luquet, C.M., Postel, U., Halperin, J., Urcola, M.R., Marques, R., Siebers, D. Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity. J. Exp. Biol. 2002;205(1):71-77.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220949_v205_n1_p71_Luquet [ ]