Artículo

Sterle, H.A.; Valli, E.; Cayrol, F.; Paulazo, M.A.; Martinel Lamas, D.J.; Diaz Flaqué, M.C.; Klecha, A.J.; Colombo, L.; Medina, V.A.; Cremaschi, G.A.; Barreiro Arcos, M.L. "Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis" (2014) Journal of Endocrinology. 222(2):243-255
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Abstract:

We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development havebeen studied, but the results are still controversial.Herein,weshowthemodulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistentwiththe rate of cell division determined by stainingtumor cellswith carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly fromthe euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increasedinhypothyroidmice. Intratumoral andperitumoral vasculogenesiswas increasedonly in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis. © 2014 Society for Endocrinology.

Registro:

Documento: Artículo
Título:Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
Autor:Sterle, H.A.; Valli, E.; Cayrol, F.; Paulazo, M.A.; Martinel Lamas, D.J.; Diaz Flaqué, M.C.; Klecha, A.J.; Colombo, L.; Medina, V.A.; Cremaschi, G.A.; Barreiro Arcos, M.L.
Filiación:Instituto de Investigaciones Biomédicas (BIOMED), Consejo Nacional de Investigaciones Científicas y Té cnicas (CONICET), Facultad de Ciencias Médicas, Pontificia Universidad Católica Argentina (UCA), Av. A. Moreau de Justo 1600, 3er piso, 1107AFF Buenos Aires, Argentina
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), CONICET, Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Area de Investigació n, Instituto de Oncología Angel H. Roffo, Universidad de Buenos Aires (UBA), CONICET, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Palabras clave:Angiogenesis; Cell cycle; T lymphoma; Thyroid hormones; beta 2 microglobulin; caspase 3; cyclin A2; cyclin B1; cyclin D1; cyclin D2; cyclin D3; cyclin dependent kinase inhibitor 1; cyclin dependent kinase inhibitor 1B; cyclin dependent kinase inhibitor 2A; cyclin dependent kinase inhibitor 2B; cyclin E; liothyronine; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; protein p53; retinoblastoma protein; thyroid hormone; thyrotropin; thyroxine; angiogenesis; animal experiment; animal tissue; article; cancer growth; cancer size; cancer survival; cell division; controlled study; female; hyperthyroidism; hypothyroidism; immunoblotting; in vivo study; liothyronine blood level; mouse; nonhuman; priority journal; protein expression; T cell lymphoma; thyroxine blood level; angiogenesis; cell cycle; T lymphoma; thyroid hormones; Animals; Apoptosis; Caspase 3; Cell Cycle Proteins; Cell Line, Tumor; Cell Proliferation; Cyclin D1; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinase Inhibitor p27; Female; Hyperthyroidism; Hypothyroidism; Lymphoma, T-Cell; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; Neovascularization, Pathologic; Proliferating Cell Nuclear Antigen; Thyroid Gland; Tumor Suppressor Protein p53
Año:2014
Volumen:222
Número:2
Página de inicio:243
Página de fin:255
DOI: http://dx.doi.org/10.1530/JOE-14-0159
Título revista:Journal of Endocrinology
Título revista abreviado:J. Endocrinol.
ISSN:00220795
CODEN:JOENA
CAS:beta 2 microglobulin, 9066-69-7; caspase 3, 169592-56-7; liothyronine, 6138-47-2, 6893-02-3; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, 210488-47-4; thyrotropin, 9002-71-5; thyroxine, 7488-70-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v222_n2_p243_Sterle

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Citas:

---------- APA ----------
Sterle, H.A., Valli, E., Cayrol, F., Paulazo, M.A., Martinel Lamas, D.J., Diaz Flaqué, M.C., Klecha, A.J.,..., Barreiro Arcos, M.L. (2014) . Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis. Journal of Endocrinology, 222(2), 243-255.
http://dx.doi.org/10.1530/JOE-14-0159
---------- CHICAGO ----------
Sterle, H.A., Valli, E., Cayrol, F., Paulazo, M.A., Martinel Lamas, D.J., Diaz Flaqué, M.C., et al. "Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis" . Journal of Endocrinology 222, no. 2 (2014) : 243-255.
http://dx.doi.org/10.1530/JOE-14-0159
---------- MLA ----------
Sterle, H.A., Valli, E., Cayrol, F., Paulazo, M.A., Martinel Lamas, D.J., Diaz Flaqué, M.C., et al. "Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis" . Journal of Endocrinology, vol. 222, no. 2, 2014, pp. 243-255.
http://dx.doi.org/10.1530/JOE-14-0159
---------- VANCOUVER ----------
Sterle, H.A., Valli, E., Cayrol, F., Paulazo, M.A., Martinel Lamas, D.J., Diaz Flaqué, M.C., et al. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis. J. Endocrinol. 2014;222(2):243-255.
http://dx.doi.org/10.1530/JOE-14-0159