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Abstract:

Histamine (HA) is a neurotransmitter synthesized in most mammalian tissues exclusively by histidine decarboxylase enzyme. Among the plethora of actions mediated by HA, the modulatory effects on steroidogenesis and proliferation in Leydig cells (LCs) have been described recently. To determine whether the effects on LCs reported could be extrapolated to all steroidogenic systems, in this study, we assessed the effect of this amine on adrenal proliferation and steroidogenesis, using two adrenocortical cell lines as experimental models, murine Y1 cells and human NCI-H295R cells. Even when steroidogenesis was not modified by HA in adrenocortical cells, the biogenic amine inhibited the proliferation of H295R cells. This action was mediated by the activation of HRH1 subtype and an increase in the production of inositol phosphates as second messengers, causing cell-cycle arrest in the G2/M phase. These results indicate a new role for HA in the proliferation of human adrenocortical cells that could contribute to a better understanding of tumor pathology as well as to the development of new therapeutic agents. © 2014 Society for Endocrinology.

Registro:

Documento: Artículo
Título:Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
Autor:Pagotto, R.M.; Pereyra, E.N.; Monzón, C.; Mondillo, C.; Pignataro, O.P.
Filiación:Laboratory of Molecular Endocrinology and Signal Transductsion, Institute of Biology and Experimental Medicine, National Research Council (IByME-CONICET), Vuelta de Obligado 2490, CP 1428 Buenos Aires, Argentina
Department of Biological Chemistry, School of Sciences, University of Buenos Aires (UBA), CP 1428 Buenos Aires, Argentina
Cell Biology Unit, Institut Pasteur de Montevideo, CP 11400 Montevideo, Uruguay
Palabras clave:Adrenocortical cells; Histamine; Proliferation; Steroidogenesis; histamine; inositol phosphate; adrenal cortex cell; animal cell; article; cell proliferation; controlled study; drug inhibition; G2 phase cell cycle checkpoint; human; human cell; Leydig cell; MTT assay; nonhuman; priority journal; steroidogenesis; Western blotting; adrenocortical cells; histamine; proliferation; steroidogenesis; Adrenal Cortex; Animals; Cell Line; Cell Proliferation; G2 Phase Cell Cycle Checkpoints; Histamine; Humans; M Phase Cell Cycle Checkpoints; Mice; Steroids
Año:2014
Volumen:221
Número:1
Página de inicio:15
Página de fin:28
DOI: http://dx.doi.org/10.1530/JOE-13-0433
Título revista:Journal of Endocrinology
Título revista abreviado:J. Endocrinol.
ISSN:00220795
CODEN:JOENA
CAS:histamine, 51-45-6, 56-92-8, 93443-21-1; inositol phosphate, 15421-51-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v221_n1_p15_Pagotto

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Citas:

---------- APA ----------
Pagotto, R.M., Pereyra, E.N., Monzón, C., Mondillo, C. & Pignataro, O.P. (2014) . Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis. Journal of Endocrinology, 221(1), 15-28.
http://dx.doi.org/10.1530/JOE-13-0433
---------- CHICAGO ----------
Pagotto, R.M., Pereyra, E.N., Monzón, C., Mondillo, C., Pignataro, O.P. "Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis" . Journal of Endocrinology 221, no. 1 (2014) : 15-28.
http://dx.doi.org/10.1530/JOE-13-0433
---------- MLA ----------
Pagotto, R.M., Pereyra, E.N., Monzón, C., Mondillo, C., Pignataro, O.P. "Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis" . Journal of Endocrinology, vol. 221, no. 1, 2014, pp. 15-28.
http://dx.doi.org/10.1530/JOE-13-0433
---------- VANCOUVER ----------
Pagotto, R.M., Pereyra, E.N., Monzón, C., Mondillo, C., Pignataro, O.P. Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis. J. Endocrinol. 2014;221(1):15-28.
http://dx.doi.org/10.1530/JOE-13-0433