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Abstract:

The present study was designed to investigate the effect of lipopolysaccharide (LPS) on the expression levels and activities of the nitric oxide synthase (NOS) and heme oxygenase (HO) systems in the rat adrenal gland. Both enzymatic activities were significantly increased in this tissue after in vivo treatment with LPS. The concurrent induction of the HO-1, NOS-1, and NOS-2 gene products was also detected as both mRNAs and protein levels were augmented by this treatment in a time-dependent way. A significant interaction between both signaling systems was also demonstrated as in vivo blockage of NOS activity with N(G)-nitro-L-arginine methyl ester (L-NAME) resulted in a significant reduction in HO expression and activity levels, while an increase in NOS activity was observed when HO was inhibited by Sn-protoporphyrin IX (Sn-PPIX). As both NOS and HO activities have been previously involved in the modulation of adrenal steroidogenesis, we investigated the participation of these signaling systems in the adrenal response to LPS. Our results showed that acute stimulation of steroid production by ACTH was significantly increased when either NOS or HO activities were inhibited. We conclude that adrenal NOS and HO can be induced by a non-lethal dose of endotoxin supporting a modulatory role for these activities in the adrenal response to immune challenges. © 2007 Society for Endocrinology.

Registro:

Documento: Artículo
Título:Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production
Autor:Grion, N.; Repetto, E.M.; Pomeraniec, Y.; Martinez Calejman, C.; Astort, F.; Sanchez, R.; Pignataro, O.P.; Arias, P.; Cymeryng, C.B.
Filiación:IBYME-CONICET Buenos Aires, Buenos Aires C1428EGA, Argentina
Departamento de Fistologia, Facultad de Medicine, Universidad de Buenos Aires, Paraguay 2155 5o, Buenos Aires C1121ABG, Argentina
Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 5o, Buenos Aires C1121ABG, Argentina
Departamento de Química Biológica, Facultad de Ciencas Exactas y Naturales, Buenos Aires C1428EGA, Argentina
Palabras clave:bacterium lipopolysaccharide; corticotropin; endotoxin; heme oxygenase; inducible nitric oxide synthase; n(g) nitroarginine methyl ester; neuronal nitric oxide synthase; protoporphyrin tin; adrenal function; animal experiment; animal tissue; article; controlled study; enzyme activity; enzyme analysis; enzyme inhibition; genetic code; hormone action; hormone determination; immunomodulation; in vivo study; male; nonhuman; priority journal; protein expression; rat; signal transduction; steroidogenesis; Adrenal Cortex; Adrenocorticotropic Hormone; Animals; Corticosterone; Enzyme Activation; Enzyme Inhibitors; Gene Expression Regulation, Bacterial; Heme Oxygenase-1; Lipopolysaccharides; Male; Metalloporphyrins; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Protoporphyrins; Random Allocation; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Stimulation, Chemical
Año:2007
Volumen:194
Número:1
Página de inicio:11
Página de fin:20
DOI: http://dx.doi.org/10.1677/JOE-06-0199
Título revista:Journal of Endocrinology
Título revista abreviado:J. Endocrinol.
ISSN:00220795
CODEN:JOENA
CAS:corticotropin, 11136-52-0, 9002-60-2, 9061-27-2; heme oxygenase, 9059-22-7; inducible nitric oxide synthase, 501433-35-8; n(g) nitroarginine methyl ester, 50903-99-6; neuronal nitric oxide synthase, 506430-87-1; protoporphyrin tin, 14325-05-4; Adrenocorticotropic Hormone, 9002-60-2; Corticosterone, 50-22-6; Enzyme Inhibitors; Heme Oxygenase-1, EC 1.14.99.3; Lipopolysaccharides; Metalloporphyrins; NG-Nitroarginine Methyl Ester, 50903-99-6; Nitric Oxide Synthase Type II, EC 1.14.13.39; Nitric Oxide Synthase, EC 1.14.13.39; Nos1 protein, rat, EC 1.14.13.39; Nos2 protein, rat, EC 1.14.13.39; Protoporphyrins; RNA, Messenger; tin protoporphyrin IX, 14325-05-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v194_n1_p11_Grion

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Citas:

---------- APA ----------
Grion, N., Repetto, E.M., Pomeraniec, Y., Martinez Calejman, C., Astort, F., Sanchez, R., Pignataro, O.P.,..., Cymeryng, C.B. (2007) . Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production. Journal of Endocrinology, 194(1), 11-20.
http://dx.doi.org/10.1677/JOE-06-0199
---------- CHICAGO ----------
Grion, N., Repetto, E.M., Pomeraniec, Y., Martinez Calejman, C., Astort, F., Sanchez, R., et al. "Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production" . Journal of Endocrinology 194, no. 1 (2007) : 11-20.
http://dx.doi.org/10.1677/JOE-06-0199
---------- MLA ----------
Grion, N., Repetto, E.M., Pomeraniec, Y., Martinez Calejman, C., Astort, F., Sanchez, R., et al. "Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production" . Journal of Endocrinology, vol. 194, no. 1, 2007, pp. 11-20.
http://dx.doi.org/10.1677/JOE-06-0199
---------- VANCOUVER ----------
Grion, N., Repetto, E.M., Pomeraniec, Y., Martinez Calejman, C., Astort, F., Sanchez, R., et al. Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production. J. Endocrinol. 2007;194(1):11-20.
http://dx.doi.org/10.1677/JOE-06-0199