Abstract:
Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. © 2001 Elsevier Science B.V.
Registro:
| Documento: |
Artículo
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| Título: | Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration |
| Autor: | Cortón, E.; Battaglini, F. |
| Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina
Departamento de Química Inorgánica, Analítica and Química Física, INQUIMAE, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón. 2, 1428 Buenos Aires, Argentina
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| Palabras clave: | Biosensors; Chronoamperometry; Cyclic voltammetry; Flow injection analysis; Redox enzymes; Cathodes; Crosslinking; Cyclic voltammetry; Electrochemical electrodes; Enzyme immobilization; Ionic strength; Osmium compounds; Proteins; Redox reactions; Cathodic currents; Biosensors |
| Año: | 2001
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| Volumen: | 511
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| Número: | 1-2
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| Página de inicio: | 8
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| Página de fin: | 12
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| DOI: |
http://dx.doi.org/10.1016/S0022-0728(01)00500-9 |
| Título revista: | Journal of Electroanalytical Chemistry
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| Título revista abreviado: | J. Electroanal. Chem.
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| ISSN: | 00220728
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220728_v511_n1-2_p8_Corton |
Referencias:
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Citas:
---------- APA ----------
Cortón, E. & Battaglini, F.
(2001)
. Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration. Journal of Electroanalytical Chemistry, 511(1-2), 8-12.
http://dx.doi.org/10.1016/S0022-0728(01)00500-9---------- CHICAGO ----------
Cortón, E., Battaglini, F.
"Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration"
. Journal of Electroanalytical Chemistry 511, no. 1-2
(2001) : 8-12.
http://dx.doi.org/10.1016/S0022-0728(01)00500-9---------- MLA ----------
Cortón, E., Battaglini, F.
"Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration"
. Journal of Electroanalytical Chemistry, vol. 511, no. 1-2, 2001, pp. 8-12.
http://dx.doi.org/10.1016/S0022-0728(01)00500-9---------- VANCOUVER ----------
Cortón, E., Battaglini, F. Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 2. Bienzymatic configuration. J. Electroanal. Chem. 2001;511(1-2):8-12.
http://dx.doi.org/10.1016/S0022-0728(01)00500-9
