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Abstract:

The characterization of a biosensor based on poly(allylamine) containing an osmium complex (OsPAA) in a matrix of industrial relevance, like milk, is presented. The biosensor is constructed by crosslinking an oxidase and the redox polymer (OsPAA) with poly(ethylene diglycidyl) ether (PEG). Parameters such as the optimum crosslinker concentration, working pH and temperature, and the effect of ionic strength were examined. When the electrode was immersed in milk spiked with substrate, an important decrease in the amperometric signal was observed compared with the one observed in a buffer solution at pH 7.0. The effects of different components of the milk are presented. The decay in the catalytic current is mainly due to the caseins, which insulate the osmium complex from the redox center of the oxidase. © 2001 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
Autor:Cortón, E.; Battaglini, F.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Ciudad Universitaria-Pabellon 2, 1428 Buenos Aires, Argentina
INQUIMAE — Departamento de Química Inorganica, Analítica y Química Física, Facultad de Ciencias Exactas and Naturales, Universidad de Buenos Aires, Ciudad Universitaria-Pabellon 2, 1428 Buenos Aires, Argentina
Palabras clave:Biosensors; Chronoamperometry; Cyclic voltammetry; Flow injection analysis; Redox enzymes; Crosslinking; Cyclic voltammetry; Electrochemical electrodes; Enzymes; Ionic strength; Osmium compounds; pH effects; Polyethers; Proteins; Redox reactions; Thermal effects; Buffer solutions; Biosensors
Año:2001
Volumen:511
Número:1-2
Página de inicio:1
Página de fin:7
DOI: http://dx.doi.org/10.1016/S0022-0728(01)00592-7
Título revista:Journal of Electroanalytical Chemistry
Título revista abreviado:J. Electroanal. Chem.
ISSN:00220728
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220728_v511_n1-2_p1_Corton

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Citas:

---------- APA ----------
Cortón, E. & Battaglini, F. (2001) . Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration. Journal of Electroanalytical Chemistry, 511(1-2), 1-7.
http://dx.doi.org/10.1016/S0022-0728(01)00592-7
---------- CHICAGO ----------
Cortón, E., Battaglini, F. "Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration" . Journal of Electroanalytical Chemistry 511, no. 1-2 (2001) : 1-7.
http://dx.doi.org/10.1016/S0022-0728(01)00592-7
---------- MLA ----------
Cortón, E., Battaglini, F. "Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration" . Journal of Electroanalytical Chemistry, vol. 511, no. 1-2, 2001, pp. 1-7.
http://dx.doi.org/10.1016/S0022-0728(01)00592-7
---------- VANCOUVER ----------
Cortón, E., Battaglini, F. Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration. J. Electroanal. Chem. 2001;511(1-2):1-7.
http://dx.doi.org/10.1016/S0022-0728(01)00592-7