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Abstract:

Bovine mastitis is one of the most economically deleterious diseases affecting dairy herds and results from an infection of the udder by pathogenic microorganisms such as Staphylococcus aureus, Streptococcus uberis, and Escherichia coli. The mammary gland is capable of preventing and combating bacterial infection by means of a complex network of innate and adaptive immune mechanisms. Lactoferrin is an 86-kDa protein with antibacterial activity that plays a role in the mammary gland's defense against infection. β-Lactoglobulin (β-LG) is an 18-kDa protein that is present in most mammals but is notably absent in humans, rodents, and lagomorphs. Different genetic variants of this protein exist, with β-LG A and β-LG B being the most common. In spite of being well studied, the biological function of β-LG is not thoroughly understood, and most noticeably, no reports exist on the effects of the native protein on bacterial growth. Hence, the objective of this study was to assess the potential antibacterial activity of β-LG against mastitis agents. To do this, we purified β-LG from normal bovine milk using a mild, nondenaturing method and performed in vitro growth inhibition assays with Staph. aureus, E. coli, and Strep. uberis. β-Lactoglobulin inhibited the growth of Staph. aureus and Strep. uberis but had no effect on E. coli. The antimicrobial activity against Staph. aureus and Strep. uberis was concentration dependent and was elicited by the intact protein because Tricine-sodium dodecyl sulfate-PAGE and analytical gel filtration chromatography did not reveal the presence of short degradation peptides. Analysis of the genetic variants of β-LG showed that β-LG A has higher inhibitory activity against Staph. aureus and Strep. uberis than β-LG B. Coincubation of β-LG and lactoferrin resulted in an augmented antibacterial activity against Staph. aureus, suggesting an additive effect of the proteins. This result, along with the proteins' complementary spectrum of action, suggests that β-LG and lactoferrin may complement each other in the mammary gland's defenses against bacterial infection. © 2011 American Dairy Science Association.

Registro:

Documento: Artículo
Título:Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria
Autor:Chaneton, L.; Pérez Sáez, J.M.; Bussmann, L.E.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, 1428, Buenos Aires, Argentina
Palabras clave:β-lactoglobulin; Antimicrobial; Mastitis-causing bacteria; antiinfective agent; lactoglobulin; animal; animal disease; article; bacterial count; bovine mastitis; cattle; classification; drug effect; Escherichia coli; female; genetics; growth, development and aging; microbiology; Staphylococcus aureus; Streptococcus; Animals; Anti-Infective Agents; Cattle; Colony Count, Microbial; Escherichia coli; Female; Lactoglobulins; Mastitis, Bovine; Staphylococcus aureus; Streptococcus; Bacteria (microorganisms); Bovinae; Escherichia coli; Lagomorpha; Mammalia; Rodentia; Staphylococcus aureus; Streptococcus uberis
Año:2011
Volumen:94
Número:1
Página de inicio:138
Página de fin:145
DOI: http://dx.doi.org/10.3168/jds.2010-3319
Título revista:Journal of Dairy Science
Título revista abreviado:J. Dairy Sci.
ISSN:00220302
CAS:Anti-Infective Agents; Lactoglobulins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220302_v94_n1_p138_Chaneton

Referencias:

  • Arnold, R.R., Russell, J.E., Champion, W.J., Brewer, M., Gauthier, J.J., Bactericidal activity of human lactoferrin: Differentiation from the stasis of iron deprivation (1982) Infect. Immun., 35, pp. 792-799
  • Bovenhuis, H., van Arendonk, J.A., Korver, S., Associations between milk protein polymorphisms and milk production traits (1992) J. Dairy Sci., 75, pp. 2549-2559
  • Calvinho, L., Tirante, L., Prevalencia de microorganismos patógenos de mastitis bovina y evolución del estado de salud de la glándula mamaria en Argentina en los últimos 25 años (2005) Revista FAVE, 4, pp. 29-40
  • Chaneton, L., Tirante, L., Maito, J., Chaves, J., Bussmann, L.E., Relationship between milk lactoferrin and etiological agent in the mastitic bovine mammary gland (2008) J. Dairy Sci., 91, pp. 1865-1873
  • de Jongh, H.H., Groneveld, T., de Groot, J., Mild isolation procedure discloses new protein structural properties of beta-lactoglobulin (2001) J. Dairy Sci., 84, pp. 562-571
  • Dinsmore, R.P., English, P.B., Gonzalez, R.N., Sears, P.M., Use of augmented cultural techniques in the diagnosis of the bacterial cause of clinical bovine mastitis (1992) J. Dairy Sci., 75, pp. 2706-2712
  • Ellison, R.T., Giehl, T.J., Killing of gram-negative bacteria by lactoferrin and lysozyme (1991) J. Clin. Invest., 88, pp. 1080-1091
  • Flower, D.R., North, A.C., Sansom, C.E., The lipocalin protein family: Structural and sequence overview (2000) Biochim. Biophys. Acta, 1482, pp. 9-24
  • Gigli, I., Maizon, D.O., Bussmann, L.E., Frecuencia genotípica de beta-lactoglobulina en relacion con mastitis clinica (2004) J. Basic Appl. Genet., 16, p. 125
  • Heck, J.M., Schennink, A., van Valenberg, H.J., Bovenhuis, H., Visker, M.H., van Arendonk, J.A., van Hooijdonk, A.C., Effects of milk protein variants on the protein composition of bovine milk (2009) J. Dairy Sci., 92, pp. 1192-1202
  • Hogan, J.S., Gonzáles, R.S., Harmon, R.J., Nickerson, S.C., Oliver, S.P., Pankey, J.W., Smith, K.L., (1999) Laboratory Handbook on Bovine Mastitis, , National Mastitis Council Inc., Madison, WI
  • Kontopidis, G., Holt, C., Sawyer, L., Invited review: β-Lactoglobulin: Binding properties, structure, and function (2004) J. Dairy Sci., 87, pp. 785-796
  • Kutila, T., Pyorala, S., Saloniemi, H., Kaartinen, L., Antibacterial effect of bovine lactoferrin against udder pathogens (2003) Acta Vet. Scand., 44, pp. 35-42
  • Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
  • Ouwehand, A.C., Salminen, S.J., Skurnik, M., Conway, P.L., Inhibition of pathogen adhesion by β-lactoglobulin (1997) Int. Dairy J., 7, pp. 685-692
  • Oviedo-Boyso, J., Valdez-Alarcon, J.J., Cajero-Juarez, M., Ochoa-Zarzosa, A., Lopez-Meza, J.E., Bravo-Patino, A., Baizabal-Aguirre, V.M., Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis (2007) J. Infect., 54, pp. 399-409
  • Pan, Y., Shiell, B., Wan, J., Coventry, M.J., Michalsky, W.P., Lee, A., Roginsky, H., The molecular characterisation and antimicrobial properties of amidated bovine b-lactoglobulin (2007) Int. Dairy J., 17, pp. 1450-1459
  • Pellegrini, A., Hulsmeier, A.J., Hunziker, P., Thomas, U., Proteolytic fragments of ovalbumin display antimicrobial activity (2004) Biochim. Biophys. Acta, 1672, pp. 76-85
  • Pérez, M.D., Calvo, M., Interaction of beta-lactoglobulin with retinol and fatty acids and its role as a possible biological function for this protein: A review (1995) J. Dairy Sci., 78, pp. 978-988
  • Pérez, M.D., Sanchez, L., Aranda, P., Ena, J.M., Oria, R., Calvo, M., Synthesis and evolution of concentration of beta-lactoglobulin and alpha-lactalbumin from cow and sheep colostrum and milk throughout early lactation (1990) Cell. Mol. Biol., 36, pp. 205-212
  • Pérez, M.D., Sanchez, L., Aranda, P., Ena, J.M., Oria, R., Calvo, M., Effect of beta-lactoglobulin on the activity of pregastric lipase. A possible role for this protein in ruminant milk (1992) Biochim. Biophys. Acta, 1123, pp. 151-155
  • Piccinini, R., Binda, E., Belotti, M., Casirani, G., Zecconi, A., The evaluation of non-specific immune status of heifers in field conditions during the periparturient period (2004) Vet. Res., 35, pp. 539-550
  • Piccinini, R., Binda, E., Belotti, M., Dapra, V., Zecconi, A., Evaluation of milk components during whole lactation in healthy quarters (2007) J. Dairy Res., 74, pp. 226-232
  • Qin, B.Y., Bewley, M.C., Creamer, L.K., Baker, E.N., Jameson, G.B., Functional implications of structural differences between variants A and B of bovine beta-lactoglobulin (1999) Protein Sci., 8, pp. 75-83
  • Rainard, P., Bacteriostatic activity of bovine milk lactoferrin against mastitic bacteria (1986) Vet. Microbiol., 11, pp. 387-392
  • Sawyer, L., Kontopidis, G., The core lipocalin, bovine beta-lactoglobulin (2000) Biochim. Biophys. Acta, 1482, pp. 136-148
  • Schägger, H., von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa (1987) Anal. Biochem., 166, pp. 368-379
  • Schukken, Y.H., Grommers, F.J., Smit, J.A., Vandegeer, D., Brand, A., Effect of freezing on bacteriologic culturing of mastitis milk samples (1989) J. Dairy Sci., 72, pp. 1900-1906
  • Seegers, H., Fourichon, C., Beaudeau, F., Production effects related to mastitis and mastitis economics in dairy cattle herds (2003) Vet. Res., 34, pp. 475-491
  • Sordillo, L.M., Contreras, G.A., Aitken, S.L., Metabolic factors affecting the inflammatory response of periparturient dairy cows (2009) Anim. Health Res. Rev., 10, pp. 53-63
  • Sordillo, L.M., Shafer-Weaver, K., DeRosa, D., Immunobiology of the mammary gland (1997) J. Dairy Sci., 80, pp. 1851-1865
  • Sordillo, L.M., Streicher, K.L., Mammary gland immunity and mastitis susceptibility (2002) J. Mammary Gland Biol. Neoplasia, 7, pp. 135-146
  • Spik, G., Cheron, A., Montreuil, J., Dolby, J.M., Bacteriostasis of a milk-sensitive strain of Escherichia coli by immunoglobulins and iron-binding proteins in association (1978) Immunology, 35, pp. 663-671
  • Watts, J.L., Etiological agents of bovine mastitis (1988) Vet. Microbiol., 16, pp. 41-66
  • Ye, X., Yoshida, S., Ng, T.B., Isolation of lactoperoxidase, lactoferrin, alpha-lactalbumin, beta-lactoglobulin B and beta-lactoglobulin A from bovine rennet whey using ion exchange chromatography (2000) Int. J. Biochem. Cell Biol., 32, pp. 1143-1150

Citas:

---------- APA ----------
Chaneton, L., Pérez Sáez, J.M. & Bussmann, L.E. (2011) . Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria. Journal of Dairy Science, 94(1), 138-145.
http://dx.doi.org/10.3168/jds.2010-3319
---------- CHICAGO ----------
Chaneton, L., Pérez Sáez, J.M., Bussmann, L.E. "Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria" . Journal of Dairy Science 94, no. 1 (2011) : 138-145.
http://dx.doi.org/10.3168/jds.2010-3319
---------- MLA ----------
Chaneton, L., Pérez Sáez, J.M., Bussmann, L.E. "Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria" . Journal of Dairy Science, vol. 94, no. 1, 2011, pp. 138-145.
http://dx.doi.org/10.3168/jds.2010-3319
---------- VANCOUVER ----------
Chaneton, L., Pérez Sáez, J.M., Bussmann, L.E. Antimicrobial activity of bovine β-lactoglobulin against mastitis-causing bacteria. J. Dairy Sci. 2011;94(1):138-145.
http://dx.doi.org/10.3168/jds.2010-3319